Recombinant Proteins

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WFDC2 Human, His

WAP Four-Disulfide Core Domain 2 Human Recombinant, His Tag

Recombinant WFDC2 Human, produced in E. coli, is a single, non-glycosylated polypeptide chain encompassing amino acids 31-124. It comprises 104 amino acids, including a 10 a.a N-terminal His tag, and has a calculated molecular mass of 11.3 kDa.
Shipped with Ice Packs
Cat. No.
BT26672
Source
Escherichia Coli.
Appearance
White lyophilized (freeze-dried) powder after filtration.

WFDC12 Human

WAP Four-Disulfide Core Domain 12 Human Recombinant

Recombinant WFDC12, produced in E. coli, is a single, non-glycosylated polypeptide chain composed of 111 amino acids (residues 24-111). With a molecular weight of 12.1 kDa, it features a 23 amino acid His-tag fused at its N-terminus.
Shipped with Ice Packs
Cat. No.
BT26507
Source
Escherichia Coli.
Appearance
A clear solution that has undergone sterile filtration.

WFDC2 Human

WAP Four-Disulfide Core Domain 2 Recombinant Human

Produced in E. coli, Recombinant Human WAP Four-Disulfide Core Domain 2 is a single-chain polypeptide that lacks glycosylation. This protein consists of 94 amino acids, resulting in a molecular mass of 10.0 kDa. However, it exhibits an apparent molecular mass of 16.9 kDa when analyzed using SDS-PAGE. The purification of WFDC2 is achieved through proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT26579
Source

Escherichia Coli.

Appearance

Sterile Filtered White lyophilized powder.

Definition and Classification

The WAP (Whey Acidic Protein) Four-Disulfide Core Domain, also known as the WFDC domain, is a protein domain characterized by the presence of eight conserved cysteine residues that form four disulfide bonds . This domain is found in a variety of proteins, including whey acidic protein, elafin, and secretory leukocyte protease inhibitor (SLPI) . The WFDC domain is classified as a protease inhibitor and is involved in various biological processes .

Biological Properties

Key Biological Properties: The WFDC domain proteins are known for their antiprotease, antibacterial, and immunomodulatory functions . They are ubiquitously expressed throughout the human body and play a crucial role in maintaining homeostasis .

Expression Patterns and Tissue Distribution: WFDC domain proteins are expressed in various tissues, including the lungs, reproductive organs, and skin . For example, SLPI and elafin are abundantly present in the human lung, where they protect against proteolytic damage .

Biological Functions

Primary Biological Functions: The primary functions of WFDC domain proteins include inhibition of proteases, antimicrobial activity, and modulation of immune responses . These proteins help in maintaining the integrity of tissues by preventing excessive proteolytic activity .

Role in Immune Responses and Pathogen Recognition: WFDC domain proteins play a significant role in the immune system by recognizing and neutralizing pathogens . They are involved in the innate immune response and help in the regulation of inflammation .

Modes of Action

Mechanisms with Other Molecules and Cells: WFDC domain proteins interact with various molecules and cells to exert their effects . For instance, they inhibit proteases by inserting an inhibitory loop into the active site of the protease, thereby blocking its activity .

Binding Partners and Downstream Signaling Cascades: These proteins bind to specific receptors on the surface of cells, triggering downstream signaling cascades that lead to immune responses and other biological effects .

Regulatory Mechanisms

Regulatory Mechanisms Controlling Expression and Activity: The expression and activity of WFDC domain proteins are tightly regulated at both the transcriptional and post-translational levels . Transcriptional regulation involves various transcription factors that bind to the promoter regions of WFDC genes .

Transcriptional Regulation and Post-Translational Modifications: Post-translational modifications, such as phosphorylation and glycosylation, also play a crucial role in modulating the activity of these proteins .

Applications

Biomedical Research: WFDC domain proteins are extensively studied in biomedical research due to their diverse functions and potential therapeutic applications . They are used as biomarkers for various diseases, including cancer and inflammatory conditions .

Diagnostic Tools and Therapeutic Strategies: These proteins are being explored as diagnostic tools and therapeutic agents. For example, WFDC2 is being investigated as a biomarker for ovarian cancer .

Role in the Life Cycle

Role Throughout the Life Cycle: WFDC domain proteins play a crucial role throughout the life cycle, from development to aging and disease . They are involved in various developmental processes and help in maintaining tissue integrity and immune function .

From Development to Aging and Disease: Aberrant expression of WFDC domain proteins is associated with various diseases, including cancer and chronic inflammatory conditions . Understanding their role in these processes can provide insights into potential therapeutic targets .

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