Recombinant Proteins

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PITPNB Human

Phosphatidylinositol Transfer Protein Beta Human Recombinant

Recombinant human PITPNB, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 291 amino acids, including a 20 amino acid His tag at the N-terminus (1-271 a.a.), and has a molecular weight of 33.7kDa. The protein is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT3958
Source
Escherichia Coli.
Appearance
Clear, sterile solution.

PITPNA Human

Phosphatidylinositol Transfer Protein Alpha Human Recombinant

Recombinant Human PITPNA, expressed in E. coli, is fused with a 20 amino acid His tag at its N-terminus. This results in a single, non-glycosylated polypeptide chain consisting of 290 amino acids (1-270 a.a.) with a molecular weight of 33.9kDa. The purification process of PITPNA involves proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT3842
Source
Escherichia Coli.
Appearance
A clear, colorless solution that has been sterilized through filtration.
Definition and Classification

Phosphatidylinositol transfer proteins (PITPs) are a diverse set of cytosolic phospholipid transfer proteins that are distinguished by their ability to transfer phospholipids between membranes in vitro . The human gene encoding PITPNA (Phosphatidylinositol Transfer Protein Alpha) is located on chromosome 17 . PITPs are classified into four classes based on their structure: Class I (PITPN), Class II (RDGB), Class III (SEC14), and Class IV (ML family) .

Biological Properties

PITPN proteins are lipid-binding proteins that transfer molecules of phosphatidylinositol or phosphatidylcholine between membrane surfaces . They are expressed in a wide range of tissues, including the brain, liver, and lungs . The protein is localized mainly in the cytosol and vesicles . PITPN proteins show a preference for phosphatidylinositol and phosphatidylcholine containing shorter saturated or monosaturated acyl chains .

Biological Functions

PITPN proteins play crucial roles in various biological processes, including lipid metabolism, membrane trafficking, and signal transduction . They are involved in the production of phosphatidylinositol 3,4,5-trisphosphate (PIP3) by phosphoinositide-3-kinase, which is essential for cell signaling . PITPN proteins also participate in immune responses and pathogen recognition by binding specific lipids .

Modes of Action

PITPN proteins interact with other molecules and cells by binding phosphatidylinositol and phosphatidylcholine and transferring them between membranes . They regulate the synthesis of phosphoinositides by delivering the substrate to specific membrane compartments or by potentiating the activities of lipid kinases . PITPN proteins also form complexes with other proteins to promote molecular exchange between closely opposed membranes .

Regulatory Mechanisms

The expression and activity of PITPN proteins are controlled by various regulatory mechanisms, including transcriptional regulation and post-translational modifications . The promoter regions of PITPN genes contain hormone response elements that regulate their expression in response to hormonal signals . Post-translational modifications, such as phosphorylation, also play a role in regulating the activity of PITPN proteins .

Applications

PITPN proteins have several applications in biomedical research, diagnostic tools, and therapeutic strategies . They are used as biomarkers for certain diseases, such as retinal degeneration and Brunner syndrome . PITPN antibodies are employed in various scientific applications, including Western Blot, Immunocytochemistry, ELISA, and Immunoprecipitation . Additionally, PITPN proteins are being explored as potential targets for therapeutic interventions in lipid-related disorders .

Role in the Life Cycle

PITPN proteins play essential roles throughout the life cycle, from development to aging and disease . They are involved in the development of the nervous system and the regulation of lipid metabolism . Mutations in PITPN genes can lead to developmental abnormalities and neurological disorders. PITPN proteins also contribute to the maintenance of cellular functions and the prevention of age-related diseases .

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