Recombinant Proteins

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SNAP25 Human

Synaptosomal-associated protein 25kda Human Recombinant

Recombinant human SNAP25, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 206 amino acids with a molecular weight of 23 kDa.
Shipped with Ice Packs
Cat. No.
BT6391
Source
Escherichia Coli.
Appearance
A clear, sterile-filtered solution.

SNAP25 Human, His

Synaptosomal-associated protein 25kDa Human Recombinant, His Tag

Recombinant Human SNAP25, with an N-terminal His-tag, is produced in E. coli. It's a single, non-glycosylated polypeptide chain with a molecular weight of 25.4 kDa, comprising 226 amino acids.
Shipped with Ice Packs
Cat. No.
BT6431
Source
Escherichia Coli.
Appearance
Clear, colorless, and sterile-filtered solution.

NAPA Human

N-Ethylmaleimide-Sensitive Factor Attachment Protein, Alpha Human Recombinant

Recombinant human NAPA, expressed in E. coli, is a single polypeptide chain devoid of glycosylation. It comprises 315 amino acids, encompassing residues 1 to 295, and has a molecular weight of 35.3 kDa. A 20 amino acid Histidine tag is attached to the N-terminus of the protein to facilitate purification by standard chromatography techniques.
Shipped with Ice Packs
Cat. No.
BT6204
Source
Escherichia Coli.
Appearance
The product is a clear, colorless, and sterile filtered solution.

SNAP23 Human

Synaptosomal-associated protein 23 Human Recombinant

Recombinant human SNAP23, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 211 amino acids. It has a molecular weight of 23.3 kDa.
Shipped with Ice Packs
Cat. No.
BT6252
Source
Escherichia Coli.
Appearance
Clear, colorless, and sterile-filtered solution.

SNAP25 C.elegans

Synaptosomal-associated protein 25kDa C.elegans Recombinant

This recombinant C. elegans SNAP-25 protein, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It comprises 207 amino acids, resulting in a molecular weight of 23kDa. The SNAP-25 gene was amplified from C. elegans using PCR and subsequently cloned into an E. coli expression vector. Purification of the SNAP-25 protein was achieved through standard chromatography methods.
Shipped with Ice Packs
Cat. No.
BT6328
Source
Escherichia Coli.
Appearance
A clear, colorless solution that has been sterilized by filtration.

SNAPIN Human

SNAP Associated Protein Human Recombinant

Recombinant Human SNAPIN, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 156 amino acids (residues 1-136) with a molecular weight of 17 kDa. The protein is expressed with a 20 amino acid His Tag at the N-terminus and purified using standard chromatography techniques.
Shipped with Ice Packs
Cat. No.
BT6502
Source
Escherichia Coli.
Appearance
The product is a sterile-filtered solution, colorless and transparent.
Definition and Classification

Synaptosomal-Associated Protein 25 kDa (SNAP-25) is a member of the Soluble NSF Attachment Protein Receptor (SNARE) protein family. It is encoded by the SNAP25 gene located on chromosome 20p12.2 in humans . SNAP-25 is a t-SNARE protein that plays a crucial role in the regulation of synaptic vesicle exocytosis .

Biological Properties

Key Biological Properties: SNAP-25 is a component of the SNARE complex, which is essential for the fusion of synaptic vesicles with the presynaptic membrane . It exists in two isoforms, SNAP-25a and SNAP-25b, which are differentially expressed during development .

Expression Patterns and Tissue Distribution: SNAP-25 is predominantly expressed in the nervous system, particularly in the brain. It is highly expressed in regions such as the cerebellar cortex, cerebellar hemisphere, and superior frontal gyrus . The expression pattern changes during development, with SNAP-25a being expressed at the embryonic stage and SNAP-25b becoming the major isoform postnatally .

Biological Functions

Primary Biological Functions: SNAP-25 is primarily involved in the exocytotic release of neurotransmitters during synaptic transmission . It mediates the apposition of synaptic vesicles to the presynaptic membrane, allowing their Ca²⁺-triggered fusion .

Role in Immune Responses and Pathogen Recognition: While SNAP-25’s primary role is in neurotransmission, it has also been implicated in various brain diseases, including Attention Deficit Hyperactivity Disorder (ADHD), schizophrenia, and bipolar disorder . Its role in immune responses and pathogen recognition is less well-defined but may be linked to its involvement in synaptic plasticity and neuronal signaling .

Modes of Action

Mechanisms with Other Molecules and Cells: SNAP-25 interacts with other SNARE proteins, such as syntaxin-1 and synaptobrevin/VAMP, to form the SNARE complex . This complex is crucial for the fusion of synaptic vesicles with the presynaptic membrane .

Binding Partners and Downstream Signaling Cascades: SNAP-25 binds to various calcium channel subunits, modulating intracellular calcium dynamics . It also interacts with proteins involved in postsynaptic receptor trafficking and spine morphogenesis .

Regulatory Mechanisms

Transcriptional Regulation: The expression of SNAP-25 is regulated at the transcriptional level, with different isoforms being expressed during different stages of development .

Post-Translational Modifications: SNAP-25 undergoes various post-translational modifications, including palmitoylation, which is essential for its proper targeting and function . These modifications can affect its interaction with other proteins and its role in synaptic transmission .

Applications

Biomedical Research: SNAP-25 is widely studied in the context of synaptic transmission and neurological disorders . It serves as a model protein for understanding the mechanisms of neurotransmitter release and synaptic plasticity .

Diagnostic Tools: SNAP-25 levels in cerebrospinal fluid have been proposed as a biomarker for synaptic damage and cognitive decline in Alzheimer’s disease .

Therapeutic Strategies: Targeting SNAP-25 and its interactions with other SNARE proteins is being explored as a potential therapeutic strategy for various neurological disorders .

Role in the Life Cycle

Development: SNAP-25 plays a critical role in the development of the nervous system, particularly in the formation and function of synapses . Its expression pattern changes during development, with different isoforms being expressed at different stages .

Aging and Disease: Alterations in SNAP-25 expression and function have been linked to various neurological disorders, including ADHD, schizophrenia, and Alzheimer’s disease . Its role in synaptic plasticity and neurotransmitter release makes it a key player in the aging brain and in the pathogenesis of neurodegenerative diseases .

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