Recombinant Proteins

p53
LBP
CEA
HLA
TCL
TTC
NPM
MAF
Bax
BID

GTF2H5 Human

General Transcription Factor IIH Polypeptide 5 Human Recombinant

Recombinant human GTF2H5, expressed in E. coli, is a non-glycosylated polypeptide chain consisting of 94 amino acids (1-71 a.a) with a molecular weight of 10.4 kDa. The protein contains a 23 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT11024
Source
Escherichia Coli.
Appearance
Clear, sterile-filtered solution.

GTF3C6 Human

General Transcription Factor IIIC Polypeptide 6 Human Recombinant

Recombinant human GTF3C6, expressed in E. coli, is a single, non-glycosylated polypeptide chain with a molecular weight of 26.4 kDa. It consists of 236 amino acids, with amino acids 1-213 representing the GTF3C6 sequence. A 23-amino acid His-tag is fused to the N-terminus to facilitate purification, which is performed using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT11116
Source
Escherichia Coli.
Appearance
A clear solution, sterilized by filtration.

GTF2A1 Human

General Transcription Factor IIA, 1 Human Recombinant

Recombinantly produced in E. coli, GTF2A1 Human is a single polypeptide chain consisting of 297 amino acids (residues 1-274) with a molecular weight of 32.2kDa. The protein includes a 23 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT10714
Source
Escherichia Coli.
Appearance
A clear solution that has been sterilized by filtration.

GTF2B Human

General Transcription Factor IIB Human Recombinant

Recombinant human GTF2B, produced in E. coli, is a non-glycosylated polypeptide chain with 336 amino acids (1-316 a.a.) and a molecular weight of 36.9 kDa. A 20 amino acid His Tag is fused to the N-terminus of GTF2B, which is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT10803
Source
Escherichia Coli.
Appearance
The product is a colorless solution that has been sterilized by filtration.

GTF2E2 Human

General Transcription Factor IIE, Polypeptide 2 Human Recombinant

Recombinant human GTF2E2, produced in E. coli, is a single polypeptide chain. It consists of 315 amino acids (1-291) and has a molecular weight of 35.6kDa. A 24 amino acid His-tag is fused to the N-terminus of GTF2E2. The protein is purified using proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT10869
Source
E.coli.
Appearance
A clear, colorless solution that has been sterilized by filtration.

GTF2F2 Human

General Transcription Factor IIF, Polypeptide 2 Human Recombinant

This product consists of the recombinant human GTF2F2 protein, produced in E. coli. It is a single polypeptide chain that is not glycosylated, containing 269 amino acids (specifically, amino acids 1 to 249) with a molecular mass of 30.5 kDa. Note that the molecular weight observed on SDS-PAGE will be higher due to the addition of a 20 amino acid His-tag at the N-terminus. This tag facilitates purification using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT10932
Source
Escherichia Coli.
Appearance
The product is a sterile, filtered solution that is colorless.
Definition and Classification

General transcription factors (GTFs) are essential proteins that facilitate the transcription of genetic information from DNA to messenger RNA (mRNA). They are crucial for the initiation of transcription by RNA polymerase II. GTFs are classified into several types based on their roles and functions, including TFIIA, TFIIB, TFIID, TFIIE, TFIIF, and TFIIH.

Biological Properties

Key Biological Properties: GTFs are characterized by their ability to bind to specific DNA sequences and recruit RNA polymerase II to the promoter region of genes. They possess DNA-binding domains and activation domains that interact with other transcription factors and coactivators.

Expression Patterns: GTFs are ubiquitously expressed in all eukaryotic cells, reflecting their fundamental role in gene expression.

Tissue Distribution: While GTFs are present in all tissues, their activity and expression levels can vary depending on the tissue type and the specific gene being transcribed.

Biological Functions

Primary Biological Functions: The primary function of GTFs is to facilitate the assembly of the pre-initiation complex (PIC) at the promoter region of genes, enabling the transcription of DNA into mRNA.

Role in Immune Responses: GTFs play a role in the regulation of genes involved in immune responses, including those encoding cytokines and other immune-related proteins.

Pathogen Recognition: GTFs can influence the expression of genes involved in pathogen recognition and the subsequent immune response, thereby contributing to the body’s defense mechanisms.

Modes of Action

Mechanisms with Other Molecules and Cells: GTFs interact with various molecules, including other transcription factors, coactivators, and chromatin remodelers, to regulate gene expression.

Binding Partners: GTFs bind to specific DNA sequences known as promoter regions, as well as to other proteins that are part of the transcription machinery.

Downstream Signaling Cascades: The binding of GTFs to DNA and their interaction with RNA polymerase II initiate a cascade of events that lead to the transcription of target genes.

Regulatory Mechanisms

Regulatory Mechanisms Controlling Expression and Activity: The expression and activity of GTFs are tightly regulated at multiple levels, including transcriptional regulation, post-transcriptional modifications, and protein-protein interactions.

Transcriptional Regulation: The expression of GTFs is controlled by various transcription factors and regulatory elements within their promoter regions.

Post-Translational Modifications: GTFs can undergo post-translational modifications, such as phosphorylation, acetylation, and ubiquitination, which can modulate their activity and stability.

Applications

Biomedical Research: GTFs are extensively studied in biomedical research to understand their role in gene regulation and their implications in various diseases.

Diagnostic Tools: GTFs can serve as biomarkers for certain diseases, and their expression levels can be used in diagnostic assays.

Therapeutic Strategies: Targeting GTFs and their regulatory pathways holds potential for the development of novel therapeutic strategies for diseases such as cancer and autoimmune disorders.

Role in the Life Cycle

Role Throughout the Life Cycle: GTFs play a critical role throughout the life cycle, from development to aging. They are involved in the regulation of genes essential for development, differentiation, and cellular homeostasis.

Development: During development, GTFs regulate the expression of genes required for cell proliferation, differentiation, and organogenesis.

Aging and Disease: Dysregulation of GTFs can contribute to aging and the development of various diseases, including cancer, neurodegenerative disorders, and metabolic diseases.

© Copyright 2024 Thebiotek. All Rights Reserved.