Recombinant Proteins

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CRISP3 Human

Cysteine-Rich Secretory Protein 3 Human Recombinant

Recombinant human CRISP3, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 235 amino acids (21-245). It includes a 10 amino acid His tag located at the N-terminus. The calculated molecular mass of this protein is 26.74 kDa.
Shipped with Ice Packs
Cat. No.
BT26288
Source
Escherichia Coli.
Appearance
A clear, colorless solution after filtration.

CRISP1 Human

Cysteine-Rich Secretory Protein 1 Human Recombinant

CRISP1 Protein, Human, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 238 amino acids (Lys 22 - Lys 249). It has a molecular weight of 27 kDa. For purification and detection purposes, a 10 amino acid His-tag is fused to the N-terminus of the protein. The protein undergoes purification using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT26116
Source
Escherichia Coli.
Appearance
Sterile Filtered White lyophilized powder.

CRISP2 Human

Cysteine-Rich Secretory Protein 2 Human Recombinant

Recombinant human CRISP2, expressed in E. coli, is a single, non-glycosylated polypeptide chain encompassing amino acids 22-243. This chain consists of 232 amino acids, including a 10 amino acid His tag located at the N-terminus. The calculated molecular mass of this protein is 26.32kDa.
Shipped with Ice Packs
Cat. No.
BT26203
Source
Escherichia Coli.
Appearance
A clear and colorless solution after filtration.
Definition and Classification

Cysteine-Rich Secretory Proteins (CRISPs) are a group of glycoproteins characterized by their high cysteine content, which forms disulfide bonds crucial for their structure and function . They belong to the CAP superfamily, which includes CRISP, antigen 5, and pathogenesis-related 1 (PR-1) proteins . CRISPs are found in various organisms, including mammals and snakes, and play significant roles in reproduction and venom toxicity .

Biological Properties

CRISPs exhibit several key biological properties:

  • Expression Patterns: CRISPs are predominantly expressed in the male reproductive tract, particularly in the testes and epididymis .
  • Tissue Distribution: They are found in spermatozoa, seminal fluid, and various snake venoms .
  • Stability: Their structure is stabilized by multiple disulfide bonds, providing high thermal and chemical stability .
Biological Functions

CRISPs have diverse biological functions:

  • Reproduction: They are involved in spermatogenesis, sperm motility, and fertilization . CRISP2, for example, plays a role in sperm-egg fusion .
  • Immune Responses: Some CRISPs are implicated in immune defense mechanisms .
  • Pathogen Recognition: They may also participate in recognizing and neutralizing pathogens .
Modes of Action

CRISPs interact with other molecules and cells through various mechanisms:

  • Binding Partners: They bind to ion channels and other proteins, regulating their activity .
  • Downstream Signaling Cascades: CRISPs can influence signaling pathways involved in capacitation, acrosome reaction, and hyperactivation of sperm .
Regulatory Mechanisms

The expression and activity of CRISPs are tightly regulated:

  • Transcriptional Regulation: Gene expression is controlled at the transcriptional level, ensuring tissue-specific expression .
  • Post-Translational Modifications: CRISPs undergo glycosylation and other modifications that affect their stability and function .
Applications

CRISPs have several applications in biomedical research:

  • Diagnostic Tools: They can be used as biomarkers for reproductive health and certain cancers .
  • Therapeutic Strategies: CRISPs are being explored as targets for infertility treatments and contraceptives .
Role in the Life Cycle

CRISPs play roles throughout the life cycle:

  • Development: They are crucial for the development and maturation of sperm .
  • Aging and Disease: Dysregulation of CRISP expression is associated with reproductive aging and diseases such as cancer .
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