Recombinant Proteins
Product List
EPHB4 Human
EPH Receptor B4 Human Recombinant
Produced in Sf9 insect cells using a baculovirus expression system, the recombinant EPHB4 protein consists of amino acids 16-539 of the human EPHB4 protein sequence (accession number: a.a.). It is a single, glycosylated polypeptide chain with an 8 amino acid Histidine tag fused at the C-terminus, resulting in a total of 532 amino acids and a molecular weight of 58.1 kDa. Under reducing conditions on SDS-PAGE, EPHB4 appears as multiple bands between 50-70 kDa due to glycosylation. The protein has been purified using proprietary chromatographic techniques.
Sf9, Baculovirus cells.
ESM1 Human
Endothelial Cell-Specific Molecule 1 Human Recombinant
ESM1 Human, HEK
Endothelial Cell-Specific Molecule 1 Human Recombinant, HEK
Recombinant human ESM1, produced in HEK293 cells, is a single, glycosylated polypeptide chain encompassing amino acids 20-184 (including a 10 amino acid C-terminal His tag). With a total of 175 amino acids, it has a calculated molecular mass of 19.5 kDa.
HEK293 cells.
ESM1 Human, SF9
Endothelial Cell-Specific Molecule 1 Human Recombinant, Sf9
Produced in Sf9 insect cells using a baculovirus expression system, ESM1 is a single, glycosylated polypeptide chain. This recombinant protein consists of 174 amino acids (20-184 a.a.), with a molecular weight of 19.2 kDa. However, it appears between 18-28 kDa on SDS-PAGE. The protein is expressed with a 9 amino acid His tag at the C-terminus and purified through proprietary chromatographic techniques.
Sf9, Baculovirus cells.
Introduction
Endothelial Cell-Specific Molecule 1 (ESM-1), also known as endocan, is a soluble dermatan sulfate proteoglycan primarily secreted by endothelial cells . It was first identified by French scientist Lassalle in 1996 and is classified as a member of the proteoglycan family . ESM-1 is involved in various physiological and pathological processes, including inflammation, angiogenesis, and tumor progression .
Key Biological Properties: ESM-1 is a secreted protein that plays a role in endothelial cell function and vascular biology . It is regulated by cytokines and proangiogenic molecules such as vascular endothelial growth factor (VEGF) and fibroblast growth factor (FGF) .
Expression Patterns: ESM-1 is predominantly expressed in endothelial cells of the lung and kidney tissues . Its expression is upregulated in response to inflammatory cytokines and other stimuli .
Tissue Distribution: ESM-1 is mainly found in the endothelial cells of the lungs and kidneys, but it is also present in other tissues under pathological conditions .
Primary Biological Functions: ESM-1 plays a crucial role in modulating endothelial cell function, vascular permeability, and leukocyte migration . It is involved in the regulation of cell proliferation, differentiation, and migration .
Role in Immune Responses: ESM-1 is implicated in immune responses by modulating leukocyte adhesion and migration . It also plays a role in pathogen recognition and the inflammatory response .
Pathogen Recognition: ESM-1 can bind to various pathogens and participate in the immune response by facilitating their clearance .
Mechanisms with Other Molecules and Cells: ESM-1 interacts with various molecules, including integrins and growth factor receptors . It modulates signaling pathways involved in cell proliferation, migration, and survival .
Binding Partners: ESM-1 binds to integrins and hepatocyte growth factor receptors, influencing cell adhesion and migration .
Downstream Signaling Cascades: ESM-1 activates downstream signaling pathways such as the PI3K/AKT pathway, which is involved in cell survival and proliferation .
Transcriptional Regulation: The expression of ESM-1 is regulated by transcription factors and cytokines . Proinflammatory cytokines such as TNF-α and IL-1β can upregulate ESM-1 expression .
Post-Translational Modifications: ESM-1 undergoes post-translational modifications, including glycosylation, which are essential for its function and stability .
Biomedical Research: ESM-1 is used as a biomarker in various diseases, including cancer, sepsis, and cardiovascular diseases . Its expression levels can provide insights into disease progression and prognosis .
Diagnostic Tools: ESM-1 levels in the blood can be measured to diagnose and monitor diseases such as cancer and sepsis .
Therapeutic Strategies: Targeting ESM-1 has potential therapeutic applications in cancer treatment, as it is involved in tumor angiogenesis and metastasis . Inhibitors of ESM-1 are being explored as potential cancer therapies .
Development: ESM-1 plays a role in vascular development and angiogenesis during embryogenesis .
Aging: The expression of ESM-1 may change with aging, influencing vascular function and the development of age-related diseases .
Disease: ESM-1 is implicated in various diseases, including cancer, cardiovascular diseases, and inflammatory conditions . Its dysregulation can contribute to disease progression and severity .
