Recombinant Proteins

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TCEAL8 Human

Transcription Elongation Factor A (SII)-Like 8 Human Recombinant

Recombinant TCEAL8 Human, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 125 amino acids (specifically, amino acids 1-117). It has a molecular mass of 14.7 kDa. However, the molecular weight observed on SDS-PAGE might appear higher. This TCEAL8 protein is fused to an 8 amino acid His-tag at the C-terminus and purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT15309
Source
E.coli.
Appearance
A clear, sterile-filtered solution.

TCEB1 Human

Transcription Elongation Factor B Polypeptide 1 Human Recombinant

Recombinantly produced in E.Coli, TCEB1 Human Recombinant is a single, non-glycosylated polypeptide chain. This protein consists of 132 amino acids, encompassing residues 1-112, and exhibits a molecular mass of 14.6kDa. A 20 amino acid His-tag is fused to the N-terminus of TCEB1. Purification is achieved through proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT15425
Source
Escherichia Coli.
Appearance
A clear, colorless solution that has been sterilized by filtration.

ELOB Mouse

Elongin B Mouse Recombinant

Recombinant Mouse ELOB, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 141 amino acids (1-118 a.a.). This protein has a molecular mass of 15.6 kDa. A 20 amino acid His-tag is fused to the N-terminus of the recombinant Mouse ELOB. Purification is achieved through proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT14797
Source
Escherichia Coli.
Appearance
Sterile, colorless solution.

ELOB Human

Elongin-B Polypeptide 2 Human Recombinant

ELOB Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 118 amino acids (1-118) and having a molecular mass of 13.1 kDa. ELOB is purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT14848
Source
Escherichia Coli.
Appearance
Sterile Filtered colorless solution.

TCEA1 Human

Transcription Elongation Factor A (SII)-1 Human Recombinant

Recombinant human TCEA1, expressed in E. coli, is a single polypeptide chain with a molecular weight of 36.5 kDa. The protein consists of 325 amino acids, spanning residues 1-301, and includes an N-terminal 24-amino acid His-tag for purification. Purification is achieved through proprietary chromatographic methods, resulting in a highly pure protein product.
Shipped with Ice Packs
Cat. No.
BT14893
Source
E.coli.
Appearance
Clear, colorless solution, sterile-filtered.

TCEAL3 Human

Transcription Elongation Factor A (SII)-Like 3 Human Recombinant

Recombinant human TCEAL3, produced in E. coli, is a single polypeptide chain with a molecular weight of 25.0 kDa. It comprises 224 amino acids, including a 24 amino acid His-tag fused at the N-terminus (amino acids 1-200). The protein has been purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT15098
Source
E.coli.
Appearance
Clear, colorless solution, sterile filtered.

TCEA2 Human

Transcription Elongation Factor A (SII)-2 Human Recombinant

Recombinant human TCEA2, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 322 amino acids (specifically, amino acids 1 to 299). It has a molecular weight of 36kDa. A 23 amino acid His-tag is fused to the N-terminus of TCEA2. Purification is achieved through proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT14958
Source
E.coli.
Appearance
A clear, sterile-filtered solution.

TCEAL1 Human

Transcription Elongation Factor A (SII)-Like 1 Human Recombinant

Recombinant human TCEAL1, with an 8 amino acid His tag fused at its C-terminus, is produced in E. coli. It is a single, non-glycosylated polypeptide chain consisting of 167 amino acids (1-159 a.a.) and has a molecular weight of 19.7 kDa. The purification of TCEAL1 is achieved using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT15012
Source
Escherichia Coli.
Appearance
A clear and colorless solution, sterile-filtered.

TCEAL7 Human

Transcription Elongation Factor A (SII)-Like 7 Human Recombinant

Recombinant human TCEAL7, expressed in E. coli, is a single polypeptide chain with a molecular weight of 14.8 kDa. The protein consists of 124 amino acids, including a 24 amino acid His-tag at the N-terminus (amino acids 1-100 of the TCEAL7 sequence). It undergoes purification using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT15167
Source
E.coli.
Appearance
Clear, colorless solution that has been sterilized by filtration.
Definition and Classification

Transcription elongation factors are proteins that facilitate the elongation phase of transcription by RNA polymerase II (Pol II). They ensure the efficient and accurate synthesis of RNA by aiding the polymerase in overcoming various obstacles, such as DNA secondary structures and nucleosomes . These factors can be classified into several groups based on their functions and interactions with Pol II, including general elongation factors, chromatin modifiers, and RNA processing factors .

Biological Properties

Key Biological Properties: Transcription elongation factors are essential for the proper functioning of Pol II during the elongation phase of transcription. They help maintain the stability of the transcription complex and ensure the fidelity of RNA synthesis .

Expression Patterns: The expression of transcription elongation factors varies across different tissues and developmental stages. For example, the elongation factor TFIIS is dynamically regulated and accumulates during heat stress in plants .

Tissue Distribution: These factors are ubiquitously expressed in various tissues, reflecting their fundamental role in gene expression. However, their levels can be modulated in response to specific cellular conditions and external stimuli .

Biological Functions

Primary Biological Functions: The primary function of transcription elongation factors is to facilitate the elongation phase of transcription by assisting Pol II in synthesizing RNA. They help Pol II overcome obstacles such as DNA secondary structures and nucleosomes, ensuring efficient and accurate RNA synthesis .

Role in Immune Responses and Pathogen Recognition: Transcription elongation factors play a crucial role in regulating the expression of genes involved in immune responses and pathogen recognition. By modulating the transcription of these genes, they help the immune system respond effectively to infections and other threats .

Modes of Action

Mechanisms with Other Molecules and Cells: Transcription elongation factors interact with various molecules and cellular components to facilitate the elongation phase of transcription. For example, elongation factor Tu (EF-Tu) delivers aminoacyl-tRNA to the ribosome during translation elongation .

Binding Partners: These factors often interact with other proteins, such as chromatin modifiers and RNA processing factors, to regulate transcription elongation. For instance, the elongation factor TFIIS interacts with RNA polymerase II to enhance its nuclease activity and facilitate transcriptional reprogramming during stress responses .

Downstream Signaling Cascades: Transcription elongation factors can influence downstream signaling cascades by regulating the expression of key genes involved in various cellular processes. This regulation can impact cell growth, differentiation, and response to environmental stimuli .

Regulatory Mechanisms

Transcriptional Regulation: The expression of transcription elongation factors is tightly regulated at the transcriptional level. Various transcription factors and regulatory elements control their gene expression in response to cellular conditions and external stimuli .

Post-Translational Modifications: Post-translational modifications, such as phosphorylation and acetylation, play a crucial role in regulating the activity of transcription elongation factors. These modifications can alter their interactions with other proteins and their ability to facilitate transcription elongation .

Applications

Biomedical Research: Transcription elongation factors are valuable tools in biomedical research for studying gene expression and regulation. They can be used to investigate the mechanisms underlying various diseases and to identify potential therapeutic targets .

Diagnostic Tools: The expression levels of transcription elongation factors can serve as biomarkers for certain diseases. For example, dysregulation of these factors has been linked to various cancers and developmental disorders .

Therapeutic Strategies: Targeting transcription elongation factors holds promise for developing novel therapeutic strategies. Modulating their activity can potentially treat diseases associated with dysregulated gene expression, such as cancer and autoimmune disorders .

Role in the Life Cycle

Development: Transcription elongation factors play a critical role in regulating gene expression during development. They ensure the proper transcription of genes involved in cell growth, differentiation, and tissue formation .

Aging and Disease: The activity of transcription elongation factors can be affected by aging and disease. Dysregulation of these factors has been implicated in various age-related diseases, including neurodegenerative disorders and cancer .

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