TCEAL3 Human
Description
TCEAL3 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Q&A
Basic Research Questions
What experimental techniques are recommended for detecting TCEAL3 expression in human tissues?
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qRT-PCR for mRNA quantification using primers targeting exons 2–3 of TCEAL3 (avoiding SNPs in exon 4 ).
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Western blotting with antibodies validated against the 25 kDa TCEAL3 protein (E. coli recombinant controls recommended ).
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Immunohistochemistry in retinal ganglion cells (RGCs) or cancer tissues, with co-staining for neuronal markers (e.g., βIII-tubulin) to confirm cellular specificity .
How does TCEAL3 expression correlate with developmental stages?
TCEAL3 is developmentally upregulated in CNS neurons like RGCs. Key findings:
What are the validated transcriptional targets of TCEAL3?
TCEAL3 modulates the PI3K/mTOR pathway by suppressing ribosomal protein S6 phosphorylation (p-S6) in RGCs. Use chromatin immunoprecipitation (ChIP) with mTOR pathway promoters (e.g., RPS6KB1) to identify direct targets .
Advanced Research Questions
How to resolve contradictory data on TCEAL3’s role in cancer versus neurodegeneration?
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Context dependency: In gastric cancer, low TCEAL3 correlates with metastasis (HR = 3.53, p < 0.001 ), while in CNS injury, high TCEAL3 inhibits axon regeneration (p < 0.01 ).
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Methodological adjustments:
What are the challenges in modeling TCEAL3 knockout in vivo?
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X-chromosome localization: Hemizygous expression in males requires CRISPR/Cas9 targeting of both alleles in female models .
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Compensatory mechanisms: Co-silencing Tceal1/5/6 (Fig. 1A ) may be needed due to functional redundancy in the Bex/Tceal family.
How to design a study investigating TCEAL3’s interaction with oxidative stress pathways?
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Experimental workflow:
Methodological Considerations Table
Product Science Overview
TCEA3 is a protein-coding gene that encodes a protein involved in the transcription process. The primary function of TCEA3 is to assist RNA polymerase II in overcoming transcriptional arrest. During transcription, RNA polymerase II can sometimes pause or stall due to various obstacles, such as DNA damage or tightly bound nucleosomes. TCEA3 helps to resolve these pauses by promoting the cleavage of the nascent RNA transcript, allowing transcription to resume from the new 3’-end .
The TCEA3 gene is located on chromosome 1p36.12 in humans. It is a member of the transcription elongation factor A (SII) family, which also includes TCEA1 and TCEA2. The protein encoded by TCEA3 contains several conserved domains that are essential for its function, including the TFIIS central domain .
TCEA3 is predicted to be involved in various biological processes, including DNA-templated transcription, cell differentiation, and response to stimulus . It is predominantly located in the nucleus, where it interacts with other transcription factors and components of the transcription machinery to facilitate efficient gene expression .
Mutations or dysregulation of TCEA3 have been associated with certain diseases. For example, TCEA3 has been linked to infantile myofibromatosis, a rare condition characterized by the development of benign tumors in the skin, muscle, and bones . Understanding the role of TCEA3 in transcription elongation and its involvement in disease can provide insights into potential therapeutic targets for related conditions.
Human recombinant TCEA3 is used in various research applications to study its function and interactions with other proteins. Recombinant proteins are produced through genetic engineering techniques, allowing researchers to obtain large quantities of pure protein for experimental purposes. These studies can help elucidate the mechanisms by which TCEA3 regulates transcription elongation and its broader implications in cellular processes.
