Recombinant Proteins

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SOX2 Human

SRY (sex determining region Y)-box 2 Human Recombinant

Recombinant human SOX2 protein, expressed in E. coli, is a non-glycosylated polypeptide chain consisting of 208 amino acids (specifically, amino acids 1-200). This protein has a molecular weight of 23.1 kDa. An 8-amino acid His-Tag is fused to the C-terminus of the SOX2 protein to facilitate purification via standard chromatography techniques.
Shipped with Ice Packs
Cat. No.
BT3262
Source
Escherichia Coli.
Appearance
The product is a colorless solution that has undergone sterile filtration.

SOX2 Human, PolyR

SRY (sex determining region Y)-box 2 Human Recombinant, Polyarginine-Tag

This product is a recombinant human SOX2 protein produced in E. coli. It is a single, non-glycosylated polypeptide chain consisting of 317 amino acids, with a molecular weight of 34.3 kDa. The protein features a C-terminal poly-arginine tag and undergoes purification using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT3328
Source
Escherichia Coli.
Appearance
Clear, colorless solution, sterile-filtered.

SOX2 Human, TAT

SRY (sex determining region Y)-box 2 Human Recombinant, TAT

This product consists of recombinant human SOX2 protein produced in E. coli. It is a single, non-glycosylated polypeptide chain comprising 330 amino acids, representing 317 amino acid residues of the full-length SOX2 protein. With a molecular weight of 36kDa, the SOX2 protein is fused to a 13 amino acid TAT peptide at its C-terminus (GGYGRKKRRQRRR) and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT3393
Source
Escherichia Coli.
Appearance
The product appears as a sterile, filtered, and lyophilized (freeze-dried) powder, white in color.
Definition and Classification

The Sex Determining Region Y (SRY) gene, also known as the SRY-box, is a gene located on the Y chromosome that is crucial for the development of male phenotypes in mammals. It is classified as a transcription factor, specifically a member of the high-mobility group (HMG)-box family of DNA-binding proteins .

Biological Properties

Key Biological Properties: The SRY gene encodes a protein that binds to specific DNA sequences, bending the DNA and facilitating the transcription of other genes necessary for male development .

Expression Patterns: SRY is expressed during a critical window in embryonic development, specifically in the bipotential gonads, which can develop into either testes or ovaries .

Tissue Distribution: The expression of SRY is primarily restricted to the developing gonads, where it initiates the formation of testes .

Biological Functions

Primary Biological Functions: The primary function of the SRY gene is to trigger the development of male characteristics by initiating the formation of testes. This process involves the differentiation of Sertoli cells, which support and nourish developing sperm cells .

Role in Immune Responses and Pathogen Recognition: While SRY is not directly involved in immune responses or pathogen recognition, its role in sex determination can indirectly influence immune system development and function, as there are differences in immune responses between males and females .

Modes of Action

Mechanisms with Other Molecules and Cells: The SRY protein interacts with other transcription factors and co-factors to regulate gene expression. It binds to specific DNA sequences, bending the DNA and facilitating the transcription of target genes .

Binding Partners: SRY interacts with various proteins, including SOX9, another transcription factor critical for testis development .

Downstream Signaling Cascades: The activation of SRY leads to the upregulation of genes involved in testis formation, such as SOX9, which further activates other genes necessary for male development .

Regulatory Mechanisms

Transcriptional Regulation: The expression of SRY is tightly regulated at the transcriptional level, with specific enhancers and promoters controlling its activity .

Post-Translational Modifications: SRY undergoes various post-translational modifications, such as phosphorylation, which can influence its stability and DNA-binding activity .

Applications

Biomedical Research: SRY is a critical gene in the study of sex determination and differentiation. Understanding its function and regulation can provide insights into disorders of sex development (DSDs) and other related conditions .

Diagnostic Tools: SRY is used as a marker in genetic testing to determine the presence of the Y chromosome, which can be important in diagnosing certain genetic conditions .

Therapeutic Strategies: While direct therapeutic applications of SRY are limited, understanding its role in sex determination can inform treatments for DSDs and other conditions related to sex chromosome abnormalities .

Role in the Life Cycle

Development: SRY plays a crucial role in the early stages of embryonic development by initiating the formation of testes and subsequent male differentiation .

Aging and Disease: While SRY’s primary role is in early development, its influence can extend into later stages of life. Abnormalities in SRY function or expression can lead to various disorders of sex development and other health issues .

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