Recombinant Proteins

p53
LBP
CEA
HLA
TCL
TTC
NPM
MAF
Bax
BID

UCHL1 Mouse

Ubiquitin Carboxyl-Terminal Esterase L1 Mouse Recombinant

Recombinant UCHL1 from mouse has been produced in E. coli. This non-glycosylated polypeptide chain consists of 246 amino acids (residues 1-223a.a) and has a molecular weight of 27.2 kDa. The protein includes a 23 amino acid His-tag fused at the N-terminus. Purification is achieved through proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT23542
Source
Escherichia Coli.
Appearance
Clear, colorless solution that has been sterilized by filtration.

UCHL1 Mouse, Active

Ubiquitin Carboxyl-Terminal Esterase L1 Mouse Recombinant, Active

Recombinant UCHL1 from mouse, expressed in E. coli, is a single, non-glycosylated polypeptide chain composed of 246 amino acids (specifically, amino acids 1-223). This protein has a molecular weight of 27.2 kDa. The N-terminus of UCHL1 is fused to a 23 amino acid His-tag. Purification is achieved through proprietary chromatographic methods.

Shipped with Ice Packs
Cat. No.
BT23648
Source
Escherichia Coli.
Appearance

The product appears as a sterile, colorless solution after filtration.

UCHL1 Human

Ubiquitin Carboxyl-Terminal Hydrolase L1 Human Recombinant

This product consists of the recombinant human UCHL1 protein, produced in E. coli bacteria. It is a single polypeptide chain that lacks glycosylation modifications. The chain comprises 223 amino acids (spanning positions 1 to 223) and has a molecular mass of 24.8 kDa. It's important to note that the protein's molecular weight may appear higher when analyzed using SDS-PAGE.
Shipped with Ice Packs
Cat. No.
BT23469
Source
Escherichia Coli.
Appearance
The product appears as a clear solution that has undergone sterile filtration.
Definition and Classification

Ubiquitin carboxy-terminal hydrolase L1 (UCHL1) is a deubiquitinating enzyme that belongs to the peptidase C12 family. It hydrolyzes small C-terminal adducts of ubiquitin to generate the ubiquitin monomer . UCHL1 is also known by several other names, including HEL-117, NDGOA, PARK5, PGP 9.5, and PGP95 .

Biological Properties

Key Biological Properties: UCHL1 is highly specific to neurons and cells of the diffuse neuroendocrine system . It is abundantly present in all neurons, accounting for 1-2% of total brain protein . The enzyme contains a catalytic triad consisting of cysteine at position 90, aspartate at position 176, and histidine at position 161, which are responsible for its hydrolase activity .

Expression Patterns and Tissue Distribution: UCHL1 is predominantly expressed in neurons and testis/ovary . It is also highly expressed in several forms of cancer .

Biological Functions

Primary Biological Functions: UCHL1 plays a crucial role in the regulation of several processes, including the maintenance of synaptic function, cardiac function, inflammatory response, and osteoclastogenesis . It abrogates the ubiquitination of multiple proteins, including WWTR1/TAZ, EGFR, HIF1A, and beta-site amyloid precursor protein cleaving enzyme 1/BACE1 .

Role in Immune Responses and Pathogen Recognition: UCHL1 regulates the ability of MHC I molecules to reach cross-presentation compartments competent for generating antigen-MHC I complexes, thereby playing a role in the immune response .

Modes of Action

Mechanisms with Other Molecules and Cells: UCHL1 interacts with various molecules and cells through its deubiquitinating activity. It stabilizes mono ubiquitin, an important component of the ubiquitin proteasome system, by preventing its degradation . UCHL1 also interacts with KLF5 and stabilizes it by reducing its polyubiquitination and proteasomal degradation .

Binding Partners and Downstream Signaling Cascades: UCHL1’s interaction with KLF5 upregulates EGFR expression while downregulating ESR1 expression at both mRNA and protein levels . It also plays a role in the JAK2/STAT3 signaling pathway, which is crucial for decidualization and the modulation of decidual NK cells .

Regulatory Mechanisms

Transcriptional Regulation: The transcription of UCHL1 is regulated by TET1 and TET3, which bind to the UCHL1 promoter region, reducing methylation of associated CpG sites and enhancing UCHL1 transcription .

Post-translational Modifications: UCHL1 undergoes various post-translational modifications that regulate its activity. For example, its deubiquitinating activity is crucial for the promotion of decidualization and the modulation of decidual NK cells .

Applications

Biomedical Research: UCHL1 is extensively studied in the context of neurodegenerative disorders such as Parkinson’s disease and Alzheimer’s disease . It is also a potential therapeutic target for these diseases due to its role in stabilizing mono ubiquitin and preventing the accumulation of ubiquitinated proteins .

Diagnostic Tools: UCHL1 is used as a biomarker for various cancers, including lung adenocarcinoma . Its expression levels can improve the detection rate of clinical lung adenocarcinoma and affect drug sensitivity .

Therapeutic Strategies: UCHL1 is a potential target for therapeutic strategies aimed at treating neurodegenerative diseases and cancers. For example, targeting UCHL1 can enhance the sensitivity of tumor cells to anti-tumor drugs .

Role in the Life Cycle

Development to Aging and Disease: UCHL1 is not essential for neuronal development but is absolutely required for the maintenance of axonal integrity . Dysfunction of UCHL1 is implicated in neurodegenerative diseases such as Parkinson’s disease and Alzheimer’s disease . It plays a significant role in the regulation of protein degradation, and its dysfunction can lead to the accumulation of ubiquitinated proteins, contributing to cell degeneration .

© Copyright 2024 Thebiotek. All Rights Reserved.