Recombinant Proteins

p53
LBP
CEA
HLA
TCL
TTC
NPM
MAF
Bax
BID

NDRG1 Human

N-Myc Downstream Regulated 1 Human Recombinant

Recombinant human NDRG1, fused with an 8 amino acid His tag at the C-terminus, is produced in E.Coli. This non-glycosylated polypeptide chain comprises 402 amino acids (1-394 a.a.) and has a molecular weight of 43.9 kDa. The purification of NDRG1 is achieved through proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT19052
Source
Escherichia Coli.
Appearance
Clear, colorless solution, sterile filtered.

NDRG2 Human

N-Myc Downstream Regulated 2 Human Recombinant

Recombinant human NDRG2, expressed in E. coli, is a single polypeptide chain consisting of 381 amino acids (residues 1-357) with a molecular weight of 41.8 kDa. This protein includes a 24 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT19144
Source
E.coli.
Appearance
A clear, colorless solution that has been sterilized by filtration.

NDRG3 Human

N-Myc Downstream Regulated 3 Human Recombinant

Recombinant human NDRG3, produced in E. coli, is a single polypeptide chain consisting of 386 amino acids (residues 1-363) with a molecular weight of 42.4 kDa. The NDRG3 protein has a 23 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT19236
Source
E.coli.
Appearance
The product is a sterile, colorless solution that has been filtered for sterilization.
Definition and Classification

N-Myc Downstream Regulated Genes (NDRGs) are a family of genes that are regulated by the N-Myc oncogene, a member of the Myc family of transcription factors. The NDRG family consists of four members: NDRG1, NDRG2, NDRG3, and NDRG4 . These genes are characterized by their involvement in various cellular processes, including proliferation, differentiation, and stress responses .

Biological Properties

Key Biological Properties: NDRGs are intracellular proteins that are induced under various stress and cell growth-regulatory conditions . They are highly conserved among multicellular organisms and are predominantly cytosolic .

Expression Patterns and Tissue Distribution: NDRG1 is ubiquitously expressed in tissues in response to cellular stress signals . NDRG2 is principally expressed in astrocytic cells throughout different regions of the brain . NDRG3 and NDRG4 also have distinct expression patterns, with NDRG4 being involved in nervous system development .

Biological Functions

Primary Biological Functions: NDRGs play crucial roles in cellular differentiation, proliferation, and growth arrest . They are also involved in tumor progression and metastasis suppression .

Role in Immune Responses and Pathogen Recognition: NDRGs are implicated in the regulation of immune responses, particularly in stress responses and cellular differentiation . They also play roles in pathogen recognition through their involvement in cellular stress mechanisms .

Modes of Action

Mechanisms with Other Molecules and Cells: NDRGs interact with various molecules and cells to regulate cellular processes. For example, NDRG1 interacts with hypoxia-inducible factor 1 alpha (HIF-1α) and p53-dependent pathways .

Binding Partners and Downstream Signaling Cascades: NDRG proteins form complexes with other proteins to regulate gene expression. For instance, NDRG1 forms complexes with Myc proteins to repress their activity . These interactions lead to downstream signaling cascades that affect cellular differentiation and stress responses .

Regulatory Mechanisms

Regulatory Mechanisms Controlling Expression and Activity: The expression of NDRGs is regulated at multiple levels, including transcriptional and post-transcriptional mechanisms . NDRG1, for example, is regulated by hypoxia-inducible factors and Myc proteins .

Transcriptional Regulation and Post-Translational Modifications: NDRGs are subject to transcriptional regulation by Myc proteins and other transcription factors . Post-translational modifications, such as phosphorylation, also play a role in regulating the activity of NDRG proteins .

Applications

Biomedical Research: NDRGs are valuable in biomedical research due to their roles in cellular differentiation and tumor suppression . They are studied for their potential as biomarkers and therapeutic targets in cancer research .

Diagnostic Tools and Therapeutic Strategies: NDRGs, particularly NDRG1, are explored as diagnostic markers for various cancers . Therapeutic strategies targeting NDRGs are being developed to enhance their tumor-suppressive functions .

Role in the Life Cycle

Role Throughout the Life Cycle: NDRGs play significant roles throughout the life cycle, from development to aging and disease . During embryogenesis, NDRGs are involved in cellular differentiation and organ development . In adulthood, they continue to regulate cellular stress responses and maintain tissue homeostasis . In diseases such as cancer, NDRGs act as tumor suppressors and are involved in inhibiting metastasis .

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