Recombinant Proteins

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Product List

NAP1L1 Human

Nucleosome Assembly Protein 1-Like 1 Human Recombinant

NAP1L1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 408 amino acids (1-388 a.a.) and having a molecular mass of 47.2kDa.
NAP1L1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT20886
Source
E.coli.
Appearance
Sterile Filtered colorless solution.

NAP1L4 Human

Nucleosome Assembly Protein 1-Like 4 Human Recombinant

NAP1L4 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 398 amino acids (1-375) and having a molecular mass of 45.2 kDa.
NAP1L4 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT20968
Source
Escherichia Coli.
Appearance
Sterile Filtered colorless solution.

Introduction

Definition and Classification

Nucleosome Assembly Proteins (NAPs) are histone chaperones that play a crucial role in the assembly and disassembly of nucleosomes, which are the fundamental units of chromatin. Nucleosomes consist of DNA wrapped around histone proteins, and their proper assembly is essential for DNA packaging and regulation within the cell . NAPs are classified into various families based on their structure and function, with the NAP1 family being one of the most well-studied .

Biological Properties

NAPs exhibit several key biological properties:

  • Expression Patterns: NAPs are ubiquitously expressed in eukaryotic cells, with varying levels depending on the cell type and developmental stage .
  • Tissue Distribution: These proteins are found in all tissues, but their expression levels can be particularly high in rapidly dividing cells, such as those in the immune system and during embryonic development .
Biological Functions

NAPs serve several primary biological functions:

  • Gene Regulation: By facilitating the proper assembly of nucleosomes, NAPs play a critical role in regulating gene expression .
  • Immune Responses: NAPs are involved in the regulation of genes that are crucial for immune responses and pathogen recognition .
  • Chromatin Dynamics: They help maintain chromatin structure during DNA replication, repair, and transcription .
Modes of Action

NAPs interact with other molecules and cells through various mechanisms:

  • Binding Partners: NAPs bind to core histones (H2A, H2B, H3, H4) and possibly to linker histone H1 .
  • Downstream Signaling Cascades: By modulating chromatin structure, NAPs influence downstream signaling pathways that regulate gene expression and cellular responses .
Regulatory Mechanisms

The expression and activity of NAPs are tightly regulated:

  • Transcriptional Regulation: NAPs are regulated at the transcriptional level by various transcription factors and signaling pathways .
  • Post-Translational Modifications: NAPs undergo post-translational modifications, such as phosphorylation and acetylation, which modulate their activity and interactions with histones .
Applications

NAPs have several applications in biomedical research and therapeutic strategies:

  • Diagnostic Tools: NAPs can be used as biomarkers for certain diseases, given their role in gene regulation and chromatin dynamics .
  • Therapeutic Strategies: Targeting NAPs and their interactions with histones offers potential therapeutic strategies for diseases involving chromatin dysregulation, such as cancer .
Role in the Life Cycle

NAPs play a vital role throughout the life cycle:

  • Development: During embryonic development, NAPs are essential for the proper assembly of chromatin and regulation of gene expression .
  • Aging and Disease: Dysregulation of NAPs has been linked to aging and various diseases, including cancer and neurodegenerative disorders .
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