Recombinant Proteins

p53
LBP
CEA
HLA
TCL
TTC
NPM
MAF
Bax
BID

ANKRA2 Human

Ankyrin Repeat Family A2 Human Recombinant

Recombinant ANKRA2 protein for human use is produced in E. coli. It is a single, non-glycosylated polypeptide chain that consists of 336 amino acids (1-313a.a) and has a molecular weight of 36.7kDa. The ANKRA2 protein is fused to a 23 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT19167
Source
Escherichia Coli.
Appearance
The product is a sterile, filtered, and clear solution.

ANKRD1 Human

Ankyrin Repeat Domain 1 Human Recombinant

Recombinant human ANKRD1, expressed in E. coli, is a 38.6 kDa polypeptide chain comprising 342 amino acids (residues 1-319). It includes a 23 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT19246
Source
E.coli.
Appearance
Clear, colorless, and sterile filtered solution.

ANKRD54 Human

Ankyrin Repeat Domain 54 Protein Human Recombinant

Recombinant human ANKRD54 protein has been produced in E. coli. This protein is a single, non-glycosylated polypeptide chain consisting of 323 amino acids (specifically, amino acids 1 to 300). It has a molecular weight of 34.9 kDa. For purification purposes, a 23 amino acid His-tag has been added to the N-terminus of the ANKRD54 protein. The purification process involves proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT19331
Source
Escherichia Coli.
Appearance
The solution is clear and has been sterilized by filtration.

ASB13 Human

Ankyrin Repeat And SOCS Box Containing 13 Human Recombinant

Recombinant Human ASB13, expressed in E. coli, is a single, non-glycosylated polypeptide chain consisting of 301 amino acids (residues 1-278). It has a molecular weight of 32.4 kDa. The protein includes a 23 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT19428
Source
Escherichia Coli.
Appearance
The product is a clear solution that has been sterilized by filtration.

ASB8 Human

Ankyrin Repeat And SOCS Box Containing 8 Human Recombinant

Produced in E.coli, Recombinant Human ASB8 is a single, non-glycosylated polypeptide chain. It consists of 311 amino acids (1-288) and has a molecular weight of 34.0kDa. The protein is fused to a 23 amino acid His-tag at its N-terminus and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT19510
Source
Escherichia Coli.
Appearance
Clear solution, sterile and filtered.
Definition and Classification

The ankyrin repeat domain is a structural motif in proteins, consisting of 33 amino acids forming two alpha helices separated by loops . These domains are among the most common protein-protein interaction platforms in nature and are found in a wide variety of proteins across eukaryotes, bacteria, and archaea . Ankyrin repeat domains typically fold into a linear solenoid structure and are involved in diverse functions such as transcriptional regulation, cell cycle control, and signal transduction .

Biological Properties

Key Biological Properties: Ankyrin repeat domains are characterized by their ability to mediate protein-protein interactions. They are highly conserved and can be found in proteins with various functions .

Expression Patterns and Tissue Distribution: Ankyrin repeat-containing proteins are ubiquitously expressed in many tissues. For instance, in plants, they play crucial roles in defense responses and development . In humans, they are involved in processes such as cytoskeletal integrity and ion transport .

Biological Functions

Primary Biological Functions: Ankyrin repeat domains facilitate protein-protein interactions, which are essential for various cellular processes. They are involved in the formation of transcription complexes, initiation of immune responses, and regulation of cell cycle stages .

Role in Immune Responses and Pathogen Recognition: Ankyrin repeat proteins play a significant role in immune responses by mediating interactions between immune cells and pathogens .

Modes of Action

Mechanisms with Other Molecules and Cells: Ankyrin repeat domains interact with other proteins through hydrogen bonding, hydrophobic interactions, and electrostatic interactions . These interactions are crucial for their role in signal transduction and cellular communication.

Binding Partners and Downstream Signaling Cascades: Ankyrin repeat proteins often bind to membrane proteins and cytoskeletal elements, influencing downstream signaling pathways that regulate cellular functions .

Regulatory Mechanisms

Regulatory Mechanisms Controlling Expression and Activity: The expression of ankyrin repeat proteins is regulated by tissue-specific and developmental-stage-specific cues . Post-translational modifications, such as phosphorylation, also play a role in modulating their activity and interactions .

Transcriptional Regulation and Post-Translational Modifications: Alternative splicing and post-translational modifications like phosphorylation and ubiquitination are key mechanisms that regulate the function and localization of ankyrin repeat proteins .

Applications

Biomedical Research: Ankyrin repeat domains are used in the design of synthetic proteins for high-affinity binding applications, offering an alternative to antibodies .

Diagnostic Tools and Therapeutic Strategies: Ankyrin repeat proteins, such as ANKRD1, have potential as cardiac biomarkers for diagnosing and prognosticating heart diseases . They are also being explored for their therapeutic potential in various diseases .

Role in the Life Cycle

Role Throughout the Life Cycle: Ankyrin repeat proteins are involved in various stages of the life cycle, from development to aging. They play roles in transcription initiation, cell cycle regulation, and immune responses, which are critical throughout an organism’s life .

From Development to Aging and Disease: Defects in ankyrin repeat proteins have been associated with several human diseases, highlighting their importance in maintaining cellular functions throughout life .

© Copyright 2024 Thebiotek. All Rights Reserved.