Recombinant Proteins

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Product List

PHF11 Human

PHF11 Protein Human Recombinant

PHF11 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 351 amino acids (1-331 a.a.) and having a molecular mass of 39.7kDa.
PHF11 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT3520
Source
Escherichia Coli.
Appearance
Sterile Filtered clear solution.

PHF13 Human

PHD Finger Protein 13 Human Recombinant

PHF13 Human Recombinant produced in E. coli is a single polypeptide chain containing 323 amino acids (1-300) and having a molecular mass of 36kDa. PHF13 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT3605
Source
Escherichia Coli.
Appearance
Sterile Filtered clear solution.

Introduction

Definition and Classification

The Plant Homeodomain (PHD) finger protein is a type of zinc finger protein extensively distributed in eukaryotes. It is characterized by a conserved Cys4-His-Cys3 motif that binds zinc ions, forming a stable structure essential for its function . PHD finger proteins are classified into various subfamilies based on their sequence and structural variations .

Biological Properties

Key Biological Properties: PHD finger proteins are involved in chromatin remodeling and transcriptional regulation. They act as “readers” of histone modifications, particularly recognizing methylated lysines on histone tails .

Expression Patterns: These proteins are ubiquitously expressed across different tissues and organs, with some members showing tissue-specific expression .

Tissue Distribution: PHD finger proteins are predominantly located in the nucleus, where they interact with chromatin to regulate gene expression .

Biological Functions

Primary Biological Functions: PHD finger proteins play crucial roles in regulating gene expression, chromatin structure, and epigenetic modifications. They are involved in various biological processes, including development, differentiation, and stress responses .

Role in Immune Responses and Pathogen Recognition: Some PHD finger proteins are implicated in immune responses by modulating the expression of genes involved in pathogen recognition and defense mechanisms .

Modes of Action

Mechanisms with Other Molecules and Cells: PHD finger proteins interact with histones and other chromatin-associated proteins to regulate gene expression. They recognize specific histone modifications, such as methylated H3K4, and recruit other proteins to modulate chromatin structure and function .

Binding Partners: PHD finger proteins often form complexes with other proteins, including histone acetyltransferases (HATs), histone deacetylases (HDACs), and transcription factors .

Downstream Signaling Cascades: By binding to specific histone marks, PHD finger proteins influence downstream signaling pathways that control various cellular processes, such as cell proliferation, differentiation, and apoptosis .

Regulatory Mechanisms

Transcriptional Regulation: PHD finger proteins regulate gene expression by recognizing and binding to specific histone modifications. This binding can either activate or repress the transcription of target genes .

Post-Translational Modifications: The activity and stability of PHD finger proteins are often regulated by post-translational modifications, such as phosphorylation, ubiquitination, and sumoylation .

Applications

Biomedical Research: PHD finger proteins are valuable tools in studying epigenetic regulation and chromatin biology. They are used to investigate the mechanisms of gene expression and the role of histone modifications in various biological processes .

Diagnostic Tools: Alterations in PHD finger proteins and their binding partners are associated with several diseases, including cancer. They serve as potential biomarkers for disease diagnosis and prognosis .

Therapeutic Strategies: Targeting PHD finger proteins and their interactions with histone modifications offers potential therapeutic strategies for treating diseases, such as cancer and genetic disorders .

Role in the Life Cycle

Development: PHD finger proteins are essential for proper development and differentiation. They regulate the expression of genes involved in developmental processes, such as flowering time in plants and embryogenesis in animals .

Aging and Disease: Dysregulation of PHD finger proteins is linked to aging and various diseases, including cancer and neurodegenerative disorders. They play a role in maintaining genomic stability and regulating cellular responses to stress .

In conclusion, PHD finger proteins are versatile regulators of gene expression and chromatin structure, with significant roles in development, immune responses, and disease. Their diverse functions and regulatory mechanisms make them important targets for biomedical research and therapeutic interventions.

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