Recombinant Proteins

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IMPAD1 Human

Inositol Monophosphatase Domain Containing 1 Human Recombinant

Recombinant IMPAD1 Human protein, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 349 amino acids (34-359 a.a). With a molecular mass of 37.6 kDa, it features a 23 amino acid His-tag fused at the N-terminus. Purification is achieved through proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT23616
Source
Escherichia Coli.
Appearance
The product is a sterile, colorless solution that has been filtered.

IMPAD1 Human, Active

Inositol Monophosphatase Domain Containing 1 Human Recombinant, BioActive

IMPAD1 Human Recombinant protein, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It encompasses amino acids 34-359, resulting in a protein of 349 amino acids with a molecular weight of 37.6 kDa. This IMPAD1 protein includes a 23 amino acid His-tag fused to its N-terminus and is purified using proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT23721
Source

Escherichia Coli.

Appearance
Clear, colorless solution that has been sterilized by filtration.

IMPAD1 Mouse

Inositol Monophosphatase Domain Containing 1 Mouse Recombinant

Produced in Sf9 Insect cells, IMPAD1 is a single, glycosylated polypeptide chain comprising 332 amino acids (34-356aa). It has a molecular mass of 36.2kDa, although on SDS-PAGE, it appears between 28-40kDa. The protein is expressed with a C-terminal 6-His tag and purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT23818
Source
Sf9 Insect cells.
Appearance
The product is a sterile, colorless solution that has been filtered.

IMPAD1 Mouse Bioactive

Inositol Monophosphatase Domain Containing 1 Mouse Recombinant Bioactive

IMPAD1 Mouse Recombinant, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain containing 332 amino acids (residues 34-356) and has a molecular mass of 36.2 kDa. On SDS-PAGE, the apparent molecular size will be approximately 28-40 kDa. This recombinant protein is expressed with a 6 amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT24148
Source
Sf9, Baculovirus cells.
Appearance
A clear, colorless solution that has been sterilized by filtration.
Definition and Classification

IMPAD1, also known as Inositol Monophosphatase Domain Containing 1, is a member of the inositol monophosphatase family. This gene encodes a protein localized to the Golgi apparatus, where it catalyzes the hydrolysis of phosphoadenosine phosphate (PAP) to adenosine monophosphate (AMP) .

Biological Properties

Key Biological Properties: IMPAD1 is part of the inositol monophosphatase superfamily and is involved in crucial cellular processes such as phosphoinositide signaling.

Expression Patterns and Tissue Distribution: IMPAD1 is expressed in various tissues, with notable expression in the brain, cartilage, and other tissues. It has been associated with GRAPP type chondrodysplasia with joint dislocations .

Biological Functions

Primary Biological Functions: IMPAD1 plays a significant role in phosphoinositide signaling by hydrolyzing PAP to AMP. This function is crucial for maintaining cellular homeostasis and signaling pathways .

Role in Immune Responses and Pathogen Recognition: While specific roles in immune responses and pathogen recognition are not well-documented, its involvement in cellular signaling suggests potential indirect roles in these processes.

Modes of Action

Mechanisms with Other Molecules and Cells: IMPAD1 interacts with various molecules within the Golgi apparatus to regulate phosphoinositide signaling. It is also involved in vesicular trafficking, which is essential for cellular communication and transport .

Binding Partners and Downstream Signaling Cascades: IMPAD1’s activity influences downstream signaling cascades by regulating the levels of inositol phosphates, which are critical for various cellular functions .

Regulatory Mechanisms

Transcriptional Regulation: IMPAD1 expression is regulated at the transcriptional level by various factors, including microRNAs such as miR-200 and miR-96. These microRNAs can repress IMPAD1 expression, and their loss during epithelial-to-mesenchymal transition (EMT) leads to de-repression of IMPAD1 .

Post-Translational Modifications: Post-translational modifications of IMPAD1, such as phosphorylation, may regulate its activity and stability, although specific details are not well-documented .

Applications

Biomedical Research: IMPAD1 is a target of interest in cancer research, particularly in understanding its role in metastasis and tumor progression. It has been implicated in lung cancer invasion and metastasis .

Diagnostic Tools and Therapeutic Strategies: Antibodies targeting IMPAD1 are used in various diagnostic applications, including Western Blot, Immunohistochemistry, and ELISA. These tools help in studying IMPAD1 expression and function in different tissues .

Role in the Life Cycle

Development to Aging and Disease: IMPAD1 plays a role throughout the life cycle, from development to aging. Its expression and activity are crucial for normal cellular functions and development. Dysregulation of IMPAD1 has been linked to diseases such as chondrodysplasia and cancer .

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