Recombinant Proteins

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GPNMB Human

Glycoprotein Nmb Human Recombinant

Recombinant Human GPNMB, expressed in E. coli, is a non-glycosylated polypeptide chain comprising 476 amino acids (22-474) with a molecular weight of 53.2 kDa. This protein features a 23 amino acid His-tag at its N-terminus and undergoes purification using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT11867
Source
Escherichia Coli.
Appearance
Clear, colorless, and sterile-filtered solution.

GPNMB Human, HEK

Glycoprotein Nmb Human Recombinant, HEK

Recombinant Human GPNMB, produced in HEK cells, is a single, glycosylated polypeptide chain (amino acids 22-486) with a total of 477 amino acids. It has a calculated molecular mass of 53.7kDa but appears at approximately 97kDa on SDS-PAGE due to glycosylation. The protein consists of the GPNMB sequence fused to a 2-amino acid C-terminal linker and a 10-amino acid His tag at the C-terminus. Purification is achieved using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT11940
Source
HEK 293.
Appearance
White powder, lyophilized and sterile-filtered.

GPNMB Human, Sf9

Glycoprotein Nmb Human Recombinant, Sf9

Recombinant Human GPNMB, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain. This protein consists of 462 amino acids (22-474a.a.), has a molecular weight of 51.8kDa, and includes a 6 amino acid His tag at its C-terminus. Purification is achieved through proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT12001
Source

Sf9, Baculovirus cells.

Appearance
A clear, colorless solution that has been sterilized by filtration.
Definition and Classification

Glycoprotein Nmb (GPNMB), also known as osteoactivin, is a type I transmembrane glycoprotein. It was initially identified in a poorly metastatic melanoma cell line. GPNMB is partially localized to the cell surface, and its large N-domain can be released into the extracellular space through ectodomain shedding by ADAM10 .

Biological Properties

Key Biological Properties: GPNMB is involved in various cellular processes, including cell differentiation, tumor progression, and tissue regeneration . It has anti-inflammatory and reparative functions and is neuroprotective in animal models of neurodegenerative diseases .

Expression Patterns: GPNMB is expressed in multiple tissues such as bone, kidney, and skeletal muscle . It is also found in melanocytes, osteoclasts, osteoblasts, and dendritic cells .

Tissue Distribution: GPNMB expression has been detected in various tissues, including the brain, where it is associated with neuroinflammatory responses in conditions like Alzheimer’s disease .

Biological Functions

Primary Biological Functions: GPNMB plays a role in cell differentiation, tumor progression, and tissue regeneration . It also has anti-inflammatory and reparative functions .

Role in Immune Responses: GPNMB modulates innate and adaptive immunity by suppressing pro-inflammatory responses and promoting the recruitment and polarization of immunosuppressive cells .

Pathogen Recognition: GPNMB is involved in the neuroinflammatory response to pathogens, particularly in neurodegenerative diseases like Alzheimer’s disease .

Modes of Action

Mechanisms with Other Molecules and Cells: GPNMB interacts with other molecules and cells through its transmembrane domain and ectodomain shedding . It can bind to the CD44 receptor on tumor cells, activating them to express cytokines like IL-33 and its receptor IL-1R1L .

Binding Partners: GPNMB binds to various receptors, including CD44, and interacts with molecules involved in inflammatory and reparative processes .

Downstream Signaling Cascades: GPNMB activates downstream signaling cascades that promote cell survival, differentiation, and tissue repair .

Regulatory Mechanisms

Transcriptional Regulation: The expression of GPNMB is regulated at the transcriptional level by various factors, including inflammatory cytokines .

Post-Translational Modifications: GPNMB undergoes post-translational modifications, such as ectodomain shedding by ADAM10, which releases its large N-domain into the extracellular space .

Applications

Biomedical Research: GPNMB is studied for its role in neurodegenerative diseases, cancer, and tissue regeneration .

Diagnostic Tools: GPNMB is being investigated as a potential biomarker for Alzheimer’s disease and other neurodegenerative conditions .

Therapeutic Strategies: GPNMB has therapeutic potential in treating neurodegenerative diseases, cancer, and inflammatory conditions due to its anti-inflammatory and reparative functions .

Role in the Life Cycle

Development: GPNMB is involved in cell differentiation and tissue development .

Aging: GPNMB expression increases with age and is associated with age-related neuroinflammatory responses .

Disease: GPNMB plays a role in the progression of neurodegenerative diseases, cancer, and inflammatory conditions .

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