Recombinant Proteins

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Product List

CLIC1 Human

Chloride Intracellular Channel 1 Human Recombinant

CLIC1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 261amino acids (1-241a.a.) and having a molecular mass of 29 kDa. CLIC1 protein is fused to a 20 amino acid His tag at N-terminus and is purified by standard chromatography.
Shipped with Ice Packs
Cat. No.
BT7622
Source
Escherichia Coli.
Appearance
Sterile filtered colorless solution.

CLIC2 Human

Chloride Intracellular Channel 2 Human Recombinant

CLIC2 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 267 amino acids (1-247 a.a.) and having a molecular mass of 30.5kDa.
CLIC2 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT7694
Source
Escherichia Coli.
Appearance
Sterile Filtered colorless solution.

CLIC4 Human

Chloride Intracellular Channel 4 Human Recombinant

CLIC4 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 273 amino acids (1-253 a.a.) and having a molecular mass of 30.9kDa. The CLIC4 is purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT7770
Source
Escherichia Coli.
Appearance
Sterile Filtered colorless solution.

CLNS1A Human

Chloride Channel, Nucleotide-Sensitive, 1A Human Recombinant

CLNS1A Human Recombinant produced in E. coli is a single polypeptide chain containing 261 amino acids (1-237) and having a molecular mass of 28.8kDa (molecular size on SDS-PAGE will appear higher).
CLNS1A is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT7858
Source
Escherichia Coli.
Appearance
Sterile filtered colorless solution.

Introduction

Definition and Classification

Chloride intracellular channels (CLICs) are a family of ion channels that facilitate the transport of chloride ions across cellular membranes. These channels are unique in that they can exist in both soluble and membrane-bound forms . CLICs are classified into several types, including CLIC1, CLIC2, CLIC3, CLIC4, CLIC5, and CLIC6, each with distinct tissue distributions and functions .

Biological Properties

Key Biological Properties: CLICs are integral membrane proteins that play crucial roles in various cellular processes. They have a single transmembrane domain and can transition between soluble and membrane-associated states .

Expression Patterns and Tissue Distribution: CLICs are ubiquitously expressed in various tissues. For instance, CLIC1 is predominantly found in the cytoplasm but can translocate to the plasma membrane under certain conditions. CLIC4 is mainly located in the outer mitochondrial membrane, while CLIC5 is found in the inner mitochondrial membrane .

Biological Functions

Primary Biological Functions: CLICs are involved in stabilizing cell membrane potential, transepithelial transport, maintaining intracellular pH, cell proliferation, fluid secretion, and regulating cell volume .

Role in Immune Responses and Pathogen Recognition: CLICs play a role in immune responses by participating in the regulation of cell volume and pH, which are critical for various immune cell functions .

Modes of Action

Mechanisms with Other Molecules and Cells: CLICs interact with various molecules and cellular structures. For example, CLIC5 has been shown to function as a fusogen, facilitating membrane fusion processes .

Binding Partners and Downstream Signaling Cascades: CLICs can bind to other proteins and participate in downstream signaling pathways. For instance, CLIC4 interacts with the cytoskeleton and is involved in actin-dependent membrane shaping .

Regulatory Mechanisms

Regulatory Mechanisms Controlling Expression and Activity: The expression and activity of CLICs are regulated at multiple levels, including transcriptional regulation and post-translational modifications .

Transcriptional Regulation and Post-Translational Modifications: CLICs undergo various post-translational modifications, such as phosphorylation, which can modulate their activity and localization .

Applications

Biomedical Research: CLICs are studied for their roles in various diseases, including cardiovascular, neurodegenerative, and cancer .

Diagnostic Tools and Therapeutic Strategies: CLICs have potential applications as biomarkers for disease diagnosis and as targets for therapeutic interventions .

Role in the Life Cycle

Role Throughout the Life Cycle: CLICs are involved in various stages of the life cycle, from development to aging and disease. They play roles in cellular processes such as proliferation, differentiation, and apoptosis .

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