Recombinant Proteins
Product List
CLIC2 Human
Chloride Intracellular Channel 2 Human Recombinant
The CLIC2 protein, produced in E. coli, is a single polypeptide chain that lacks glycosylation. It comprises 267 amino acids, with amino acids 1 to 247 representing the CLIC2 sequence. The protein has a molecular weight of 30.5 kDa.
For purification purposes, a 20 amino acid His-tag is attached to the N-terminus of CLIC2. The protein is then purified using proprietary chromatographic methods.
CLIC4 Human
Chloride Intracellular Channel 4 Human Recombinant
CLNS1A Human
Chloride Channel, Nucleotide-Sensitive, 1A Human Recombinant
CLIC1 Human
Chloride Intracellular Channel 1 Human Recombinant
Introduction
Chloride intracellular channels (CLICs) are a family of ion channels that facilitate the transport of chloride ions across cellular membranes. These channels are unique in that they can exist in both soluble and membrane-bound forms . CLICs are classified into several types, including CLIC1, CLIC2, CLIC3, CLIC4, CLIC5, and CLIC6, each with distinct tissue distributions and functions .
Key Biological Properties: CLICs are integral membrane proteins that play crucial roles in various cellular processes. They have a single transmembrane domain and can transition between soluble and membrane-associated states .
Expression Patterns and Tissue Distribution: CLICs are ubiquitously expressed in various tissues. For instance, CLIC1 is predominantly found in the cytoplasm but can translocate to the plasma membrane under certain conditions. CLIC4 is mainly located in the outer mitochondrial membrane, while CLIC5 is found in the inner mitochondrial membrane .
Primary Biological Functions: CLICs are involved in stabilizing cell membrane potential, transepithelial transport, maintaining intracellular pH, cell proliferation, fluid secretion, and regulating cell volume .
Role in Immune Responses and Pathogen Recognition: CLICs play a role in immune responses by participating in the regulation of cell volume and pH, which are critical for various immune cell functions .
Mechanisms with Other Molecules and Cells: CLICs interact with various molecules and cellular structures. For example, CLIC5 has been shown to function as a fusogen, facilitating membrane fusion processes .
Binding Partners and Downstream Signaling Cascades: CLICs can bind to other proteins and participate in downstream signaling pathways. For instance, CLIC4 interacts with the cytoskeleton and is involved in actin-dependent membrane shaping .
Regulatory Mechanisms Controlling Expression and Activity: The expression and activity of CLICs are regulated at multiple levels, including transcriptional regulation and post-translational modifications .
Transcriptional Regulation and Post-Translational Modifications: CLICs undergo various post-translational modifications, such as phosphorylation, which can modulate their activity and localization .
