Recombinant Proteins

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Product List

MFAP2 Human

Microfibrillar-associated Protein 2 Human Recombinant

MFAP2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 190 amino acids (18-183 a.a) and having a molecular mass of 21.5kDa.
MFAP2 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT12098
Source
Escherichia Coli.
Appearance
Sterile Filtered clear solution.

MFAP3 Human

Microfibrillar-associated Protein 3 Human Recombinant

MFAP3 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 152 amino acids (19-147) and having a molecular mass of 16.7 kDa.
MFAP3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT12176
Source
Escherichia Coli.
Appearance
Sterile Filtered clear solution.

MFAP4 Human

Microfibrillar-associated Protein 4 Human Recombinant

MFAP4 Human Recombinant produced in E. coli is a single polypeptide chain containing 259 amino acids (22-255) and having a molecular mass of 29.2 kDa.
MFAP4 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT12258
Source
E.coli.
Appearance
Sterile Filtered colorless solution.

MFAP4 Human, Sf9

Microfibrillar-associated Protein 4 Human Recombinant, Sf9

MFAP4 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 243 amino acids (22-255a.a) and having a molecular mass of 27.5kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa). 
MFAP4 is fused to a 6 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT12352
Source
Sf9, Baculovirus cells.
Appearance
Sterile Filtered clear solution.

Introduction

Definition and Classification

Microfibrillar Associated Proteins (MFAPs) are extracellular matrix glycoproteins involved in the assembly of microfibrils, elastinogenesis, and maintaining tissue homeostasis . They are classified into five subfamily members: MFAP1, MFAP2, MFAP3, MFAP4, and MFAP5 .

Biological Properties

Key Biological Properties: MFAPs are integral components of elastic fibers and microfibrils, contributing to the structural integrity of tissues . They interact with other extracellular matrix proteins such as fibrillin and tropoelastin .

Expression Patterns and Tissue Distribution: MFAPs are highly expressed in elastin-rich tissues such as the lungs, blood vessels, and skin . MFAP4, for instance, is found in elastic fibers and interacts directly with fibrillin-1 .

Biological Functions

Primary Biological Functions: MFAPs play a crucial role in the organization of the extracellular matrix (ECM), aiding in the proper assembly of elastic fibers . They are also involved in cell adhesion and intercellular interactions .

Role in Immune Responses and Pathogen Recognition: MFAP4 has been identified as a macrophage-specific innate immune molecule in teleost fish, indicating its role in host defense .

Modes of Action

Mechanisms with Other Molecules and Cells: MFAPs bind to various ECM components, including tropoelastin and fibrillin-1 and -2 . They also interact with integrin receptors, influencing cell signaling pathways .

Binding Partners and Downstream Signaling Cascades: MFAP4 binds to tropoelastin and fibrillin, promoting elastic fiber assembly . It also interacts with RGD-dependent integrin receptors, contributing to disease development and progression .

Regulatory Mechanisms

Transcriptional Regulation: The expression of MFAP genes can be regulated by various factors. For example, the promoter of the human MFAP4 gene is a TATA-less promoter that can be up-regulated by retinol and coenzyme Q10 .

Post-Translational Modifications: MFAPs undergo various post-translational modifications that influence their function and interactions with other ECM components .

Applications

Biomedical Research: MFAPs are studied for their role in tissue engineering and regenerative medicine due to their involvement in ECM organization and elastic fiber formation .

Diagnostic Tools: Elevated levels of MFAPs, such as MFAP5, have been associated with certain cancers, making them potential biomarkers for disease diagnosis .

Therapeutic Strategies: Targeting MFAPs could offer therapeutic benefits in treating fibrotic diseases and improving wound healing outcomes .

Role in the Life Cycle

Development to Aging and Disease: MFAPs are crucial throughout the life cycle, from development to aging. They are involved in tissue development, maintenance, and repair . Dysregulation of MFAPs has been linked to various diseases, including fibrosis and cancer .

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