Recombinant Proteins

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MED20 Human

Mediator Complex Subunit 20 Human Recombinant

This product consists of the human MED20 protein, recombinantly produced in E. coli bacteria. This single-chain polypeptide comprises 235 amino acids, with the first 212 representing the MED20 sequence. It has a molecular weight of 25.6 kDa and lacks glycosylation. For purification and detection purposes, a 23-amino acid His-tag is attached to the protein's N-terminus.
Shipped with Ice Packs
Cat. No.
BT5822
Source
E.coli.
Appearance
A clear, sterile-filtered liquid.

MED21 Human

Mediator Complex Subunit 21 Human Recombinant

This product consists of the human MED21 protein, which has been recombinantly produced in E. coli bacteria. The protein is not glycosylated, meaning it does not have sugar molecules attached to it. It comprises a single polypeptide chain of 167 amino acids, with the first 144 amino acids corresponding to the MED21 sequence. A 23 amino acid His-tag is attached to the N-terminus of the protein for purification purposes. The molecular weight of the protein is 18.0 kDa. The protein has been purified using proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT5874
Source
E.coli.
Appearance
A clear and colorless solution that has been sterilized by filtration.

MED27 Human

Mediator Complex Subunit 27 Human Recombinant

Recombinant human MED27, expressed in E. coli, is a single, non-glycosylated polypeptide chain. This protein consists of 334 amino acids, with a sequence spanning residues 1-311, and has a molecular weight of 37.8 kDa. A 23 amino acid His-tag is fused to the N-terminus of MED27 to aid in purification, which is carried out using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT5955
Source
E.coli.
Appearance
A clear, colorless solution that has been sterilized by filtration.

MED4 Human

Mediator Complex Subunit 4 Human Recombinant

The MED4 protein, produced in E.Coli, is a single, non-glycosylated polypeptide chain consisting of 278 amino acids (with the sequence spanning from amino acid 1 to 270). It has a molecular weight of 30.7kDa. This MED4 protein is engineered with an 8 amino acid His Tag fused at its C-terminus to facilitate purification, which is achieved through proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT6023
Source
Escherichia Coli.
Appearance
Clear, colorless solution that has been sterilized by filtration.

MED7 Human

Mediator Complex Subunit 7 Human Recombinant

Recombinant human MED7, produced in E. coli, is a single polypeptide chain consisting of 256 amino acids (1-233) with a molecular weight of 29.7 kDa. This protein is fused to a 23 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT6065
Source
E.coli.
Appearance
Clear, sterile-filtered solution.
Definition and Classification

The Mediator complex is a multiprotein complex that functions as a transcriptional coactivator in all eukaryotes. It was discovered in 1990 in the lab of Roger D. Kornberg, who received the 2006 Nobel Prize in Chemistry for his work on the molecular basis of eukaryotic transcription . The Mediator complex interacts with transcription factors and RNA polymerase II, transmitting signals from the transcription factors to the polymerase . It is highly variable in its evolutionary, compositional, and conformational levels .

Biological Properties

Key Biological Properties: The Mediator complex is essential for the successful transcription of nearly all class II gene promoters in yeast and works similarly in mammals . It is composed of 25 subunits in yeast and up to 26 subunits in mammals .

Expression Patterns and Tissue Distribution: The Mediator complex is located within the cell nucleus and is ubiquitously expressed in various tissues . Its subunits can be differentially expressed in specific tissues or developmental stages, contributing to its diverse functions .

Biological Functions

Primary Biological Functions: The Mediator complex serves as a bridge between RNA polymerase II and transcription factors, facilitating the assembly of the pre-initiation complex (PIC) and enabling transcription initiation . It plays a crucial role in regulating gene expression in response to various signals .

Role in Immune Responses and Pathogen Recognition: The Mediator complex is involved in the transcriptional regulation of genes associated with immune responses and pathogen recognition. It helps in the activation of immune-related genes in response to environmental stresses .

Modes of Action

Mechanisms with Other Molecules and Cells: The Mediator complex interacts with various transcription factors, coactivators, and general transcription factors to regulate gene expression . It undergoes conformational changes to facilitate the assembly of the PIC and the recruitment of RNA polymerase II .

Binding Partners and Downstream Signaling Cascades: The Mediator complex binds to the C-terminal domain of RNA polymerase II holoenzyme and acts as a bridge between this enzyme and transcription factors . It also interacts with enhancer-bound coactivators to establish enhancer-promoter contact, facilitating transcriptional activation .

Regulatory Mechanisms

Transcriptional Regulation: The Mediator complex modulates the association between transcription factors and RNA polymerase II to precisely regulate gene transcription . It is involved in the regulation of enhancer-promoter interactions and the formation of transcriptional hubs .

Post-Translational Modifications: The activity of the Mediator complex is regulated by various post-translational modifications, including phosphorylation by cyclin-dependent kinases (CDKs) . These modifications can alter the conformation and function of the complex, affecting its ability to regulate gene expression .

Applications

Biomedical Research: The Mediator complex is a key target in biomedical research due to its central role in transcriptional regulation. It is studied for its involvement in various diseases, including cancer and developmental disorders .

Diagnostic Tools and Therapeutic Strategies: Understanding the function and regulation of the Mediator complex can lead to the development of diagnostic tools and therapeutic strategies. Targeting specific subunits or regulatory mechanisms of the complex may provide new avenues for treating diseases associated with dysregulated gene expression .

Role in the Life Cycle

Development to Aging and Disease: The Mediator complex plays a crucial role throughout the life cycle, from development to aging and disease. It is involved in the regulation of developmental transitions, such as vegetative phase change and floral transition in plants . In mammals, it regulates gene expression during various stages of development and is implicated in age-related diseases and cancer .

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