Recombinant Proteins

p53
LBP
CEA
HLA
TCL
TTC
NPM
MAF
Bax
BID

KRT14 Human

Cytokeratin 14 Human Recombinant

Recombinantly produced in E. coli, Cytokeratin 14 Human Recombinant is a single, non-glycosylated polypeptide chain with a molecular weight of 51,530 Daltons. The purification of KRT14 is achieved using proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT27584
Source
Escherichia Coli.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder

KRT14 Human, His

Cytokeratin 14 Human Recombinant, His Tag

Recombinant human KRT14, produced in E. coli, is a non-glycosylated polypeptide chain containing 492 amino acids (residues 1-472) with a molecular weight of 53.8 kDa. It is a single chain protein. A 20 amino acid His-tag is fused to the N-terminus of KRT14. The protein is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT27662
Source
Escherichia Coli.
Appearance
Clear, colorless, and sterile-filtered solution.

KRT16 Human

Cytokeratin 16 Human Recombinant

This recombinant KRT16 protein, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 496 amino acids (residues 1-473) with a molecular weight of 53.7 kDa. It includes an N-terminal 23 amino acid His-tag and undergoes purification via proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT27748
Source
Escherichia Coli.
Appearance
A sterile, colorless solution.

KRT8 Human, GST

Cytokeratin 8 Human Recombinant, GST Tag

This product consists of full-length recombinant human Cytokeratin 8 protein expressed in E.coli, exhibiting a molecular weight of 78 kDa as determined by SDS-PAGE. The protein is engineered with a GST-Tag and purified using proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT28621
Source
Escherichia Coli.
Appearance
A clear solution that has undergone sterile filtration.

KRT8 Human, His

Cytokeratin 8 Human Recombinant, His Tag

Recombinant human KRT8, produced in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 506 amino acids (specifically, amino acids 1-483) and has a molecular weight of 56kDa. For purification, a 23 amino acid His-tag is fused to the N-terminus, and proprietary chromatographic techniques are employed.
Shipped with Ice Packs
Cat. No.
BT28725
Source
Escherichia Coli.
Appearance
The product is a sterile, colorless solution that has been filtered for sterility.

KRT19 Human, His

Cytokeratin 19 Human Recombinant , His Tag

Recombinant human KRT19, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 423 amino acids (1-400) with a molecular mass of 46.5 kDa. It is fused to a 23 amino acid His-Tag at the N-terminus and purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT28222
Source
Escherichia Coli.
Appearance
Sterile, colorless solution.

KRT20 Human

Cytokeratin 20 Human Recombinant

Recombinant Human Cytokeratin 20, produced in E. coli, is a single, non-glycosylated polypeptide chain with a molecular weight of 48,553 Daltons. The purification of KRT20 is achieved through proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT28309
Source
Escherichia Coli.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

KRT20 Human, His

Cytokeratin 20 Human Recombinant, His Tag

Recombinant Human KRT20, produced in E. coli, is a single, non-glycosylated polypeptide chain. It comprises 447 amino acids (specifically, amino acids 1 to 424) and has a molecular weight of 50.9 kDa. This KRT20 protein is fused with a 23 amino acid His-tag at its N-terminus and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT28411
Source
Escherichia Coli.
Appearance
The product is a sterile, colorless solution that has been filtered for sterility.

KRT5 Human

Cytokeratin 5 Human Recombinant

Recombinant human KRT5 is a single, non-glycosylated polypeptide chain produced in E. coli. It contains 613 amino acids (residues 1-590) and has a molecular weight of 64.8 kDa. The protein is fused to a 23 amino acid His-tag at the N-terminus.
Shipped with Ice Packs
Cat. No.
BT28493
Source
Escherichia Coli.
Appearance
Clear, sterile-filtered solution.

KRT8 Human

Cytokeratin 8 Human Recombinant

Recombinant human Cytokeratin 8, expressed in E. coli, is a non-glycosylated polypeptide chain with a molecular weight of 53,532 Daltons. The purification of KRT8 is achieved through proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT28567
Source
Escherichia Coli.
Appearance
Sterile white powder obtained by lyophilization (freeze-drying).
Definition and Classification

Cytokeratins are a class of keratin proteins found in the intracytoplasmic cytoskeleton of epithelial tissue. They are an important component of intermediate filaments, which help cells resist mechanical stress . Cytokeratins are classified into two main types: acidic (type I) and basic or neutral (type II). Each cytokeratin pair contains both acidic and basic cytokeratins in equimolar amounts . These proteins are numbered in order of decreasing size, from high molecular weight (HMWCKs) to low molecular weight (LMWCKs) .

Biological Properties

Cytokeratins exhibit key biological properties, including providing structural support and protecting epithelial cells against mechanical stress . They are expressed in a tissue-specific manner, with different cytokeratins being present in various organs and tissues . For example, cytokeratin 5 is expressed by squamous cells throughout the body, while cytokeratin 7 is found in epithelial cells of the thyroid gland, lungs, and other organs .

Biological Functions

Cytokeratins play several primary biological functions, including maintaining the structural integrity of epithelial cells and participating in cell signaling, cell-cell adhesion, and apoptosis . They act as markers of cell proliferation and are involved in the response to stress and preservation of epithelial cell authenticity . Cytokeratins also play a role in immune responses and pathogen recognition by maintaining the barrier function of epithelial tissues .

Modes of Action

Cytokeratins interact with other molecules and cells through various mechanisms. They form heterodimeric pairs of acidic and basic subunits, which are further assembled into keratin filaments . These filaments interact with desmosomes and hemidesmosomes, contributing to cell-cell adhesion and the connection between basal cells and the underlying connective tissue . Cytokeratins also participate in signaling pathways, such as the PI3K/Akt and ERK MAPK pathways, modulating intracellular signaling .

Regulatory Mechanisms

The expression and activity of cytokeratins are regulated by several mechanisms, including transcriptional regulation and post-translational modifications . Site-specific phosphorylation plays a crucial role in the assembly and disaggregation of the keratin network, impacting dynamic cellular processes such as mitosis, cell movement, and differentiation . The expression of cytokeratins is also organ-specific and depends on the type of epithelial cells and their degree of differentiation .

Applications

Cytokeratins have various applications in biomedical research, diagnostic tools, and therapeutic strategies. They are commonly used as markers to identify and classify different types of epithelial cells, playing a key role in the diagnosis and classification of many types of cancer . Immunohistochemistry (IHC) techniques are used to detect cytokeratin expression in tissue samples, aiding in tumor diagnosis and characterization . Additionally, cytokeratins are being explored as potential therapeutic targets for certain cancers .

Role in the Life Cycle

Cytokeratins play a significant role throughout the life cycle, from development to aging and disease. They are involved in the differentiation and development of epithelial tissues and contribute to the maintenance of tissue integrity and function . During aging, changes in cytokeratin expression and function can impact tissue homeostasis and contribute to age-related diseases . In disease states, such as cancer, cytokeratins can serve as biomarkers for diagnosis and prognosis .

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