Recombinant Proteins

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LECT1 (214-333) Human

Leukocyte Cell Derived Chemotaxin 1 (214-333 a.a.) Human Recombinant

Recombinant human LECT1, encompassing amino acids 214 to 333, has been produced in E. coli. This single polypeptide chain consists of 143 amino acids and exhibits a molecular weight of 16.2 kDa. The N-terminus of LECT1 is fused with a 23-amino acid His-tag. Purification is achieved through proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT2259
Source
Escherichia Coli.
Appearance
A clear solution that has undergone sterile filtration.

LECT1 Human

Leukocyte Cell Derived Chemotaxin 1 Human Recombinant

This product contains a specifically designed version of the human LECT1 protein. It is produced in bacteria and purified to a high degree. This specific form of LECT1 contains 144 amino acids, resulting in a molecular weight of 16.3 kDa. For ease of purification and detection, it also includes a 23 amino acid tag attached to the beginning of the protein.
Shipped with Ice Packs
Cat. No.
BT2344
Source
Escherichia Coli.
Appearance
Clear liquid, free from visible particles and sterilized.

LECT2 Human

Leukocyte Cell-Derived Chemotaxin 2 Human Recombinant

Recombinant Human LECT2, expressed in E. coli, is a single, non-glycosylated polypeptide chain encompassing amino acids Gly19 to Leu151 (143 amino acids total). This includes a 10-amino acid Histidine tag located at the N-terminus. The calculated molecular mass is 16kDa.
Shipped with Ice Packs
Cat. No.
BT2437
Source
Escherichia Coli.
Appearance
White lyophilized (freeze-dried) powder after filtration.
Definition and Classification

Leukocyte Cell Derived Chemotaxin 2 (LECT2) is a protein-coding gene that encodes a secreted protein with chemotactic activity. It is also known as Chondromodulin-II (ChM-II) due to its role in promoting chondrocyte proteoglycan synthesis and cartilage growth . LECT2 belongs to the peptidase M23 family and is classified as a multifunctional secreted factor involved in various physiological and pathological processes .

Biological Properties

Key Biological Properties: LECT2 is a 16 kDa protein that acts as a chemotactic factor for neutrophils and stimulates the growth of chondrocytes and osteoblasts . It does not exhibit metalloendopeptidase activity .

Expression Patterns: LECT2 is primarily expressed in the liver, but it is also found in other tissues such as the kidney, lung, and various immune cells .

Tissue Distribution: The protein is secreted into the bloodstream and can be detected in various tissues, including the liver, kidney, and lung .

Biological Functions

Primary Biological Functions: LECT2 plays a crucial role in immune responses by mediating neutrophil migration and acting as an antiviral regulator . It is also involved in promoting chondrocyte proliferation and cartilage growth .

Role in Immune Responses: LECT2 is associated with many immune processes and immune-related diseases via its binding to cell surface receptors such as CD209a, Tie1, and Met in various cell types .

Pathogen Recognition: LECT2 enhances the RIG-I-mediated innate immune response by promoting interferon production and inhibiting viral replication .

Modes of Action

Mechanisms with Other Molecules and Cells: LECT2 binds to cell surface receptors such as CD209a, Tie1, and Met, facilitating various signaling pathways . It also interacts with the MET receptor tyrosine kinase, promoting the recruitment of phosphatase PTP4A1 to MET and protecting RIG-I from degradation .

Binding Partners: LECT2 binds to receptors like CD209a, Tie1, and Met, which are involved in immune responses and other physiological processes .

Downstream Signaling Cascades: Upon binding to its receptors, LECT2 activates various signaling pathways, including the VEGF165-VEGFR2 signaling in liver cancer and the LECT2-Tie1 signaling in liver fibrosis .

Regulatory Mechanisms

Transcriptional Regulation: The expression of LECT2 is regulated at the transcriptional level by various factors, including cytokines and growth factors .

Post-Translational Modifications: LECT2 undergoes post-translational modifications such as glycosylation, which are essential for its stability and function .

Applications

Biomedical Research: LECT2 is used as a biomarker in various diseases, including liver fibrosis, hepatic cell carcinoma, and systemic inflammatory diseases .

Diagnostic Tools: LECT2 levels can be measured in blood samples to diagnose and monitor the progression of certain diseases .

Therapeutic Strategies: Targeting LECT2 and its signaling pathways holds potential for developing therapeutic interventions for immune-related diseases and cancer .

Role in the Life Cycle

Development: LECT2 plays a role in cartilage growth and chondrocyte proliferation during development .

Aging and Disease: LECT2 is involved in various age-related diseases, including liver fibrosis and amyloidosis . Its misfolding can lead to the formation of insoluble fibrils, contributing to tissue amyloidosis .

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