Recombinant Proteins

p53
LBP
CEA
HLA
TCL
TTC
NPM
MAF
Bax
BID

ETS2 Human

V-Ets Avian Erythroblastosis Virus E26 Oncogene 2 Human Recombinant

Recombinant human ETS2, produced in E. coli bacteria, is a single polypeptide chain that lacks glycosylation modifications. It comprises 489 amino acids, spanning from position 1 to 469, and exhibits a molecular weight of 55.1 kilodaltons (kDa). For purification and detection purposes, a 20-amino acid Histidine tag is attached to the N-terminus of the ETS2 protein. The purification process involves proprietary chromatographic techniques to ensure high purity.
Shipped with Ice Packs
Cat. No.
BT8484
Source
Escherichia Coli.
Appearance
The solution is sterile-filtered, resulting in a clear and colorless liquid.
Definition and Classification

Protein C-Ets, also known as ETS1, is a transcription factor encoded by the ETS1 gene in humans. It belongs to the ETS (E26 transformation-specific) family of transcription factors, which is characterized by a conserved DNA-binding domain known as the ETS domain . The ETS family comprises 28 genes in humans and 27 in mice .

Biological Properties

Key Biological Properties: ETS1 is a transcription factor that binds to specific DNA sequences to regulate gene expression. It is involved in various cellular processes, including differentiation, proliferation, and apoptosis .

Expression Patterns: ETS1 is expressed in multiple tissues, with high levels observed in immune tissues such as the thymus, spleen, and lymph nodes . It is also expressed in endothelial cells, vascular smooth muscle cells, and epithelial cancer cells .

Tissue Distribution: ETS1 is predominantly found in immune cells, including B cells, T cells, NK cells, and non-lymphoid immune cells . It is also present in other tissues such as the liver, kidney, lung, brain, and male reproductive tissue .

Biological Functions

Primary Biological Functions: ETS1 plays a crucial role in regulating immune cell function, cellular differentiation, and tumor progression . It is involved in the regulation of genes associated with cell cycle control, cell migration, and angiogenesis .

Role in Immune Responses and Pathogen Recognition: ETS1 is essential for the development and function of natural killer (NK) cells, which are critical for immune defense against tumors and pathogens . It regulates the expression of key transcription factors that control NK cell differentiation and activation .

Modes of Action

Mechanisms with Other Molecules and Cells: ETS1 interacts with various co-regulatory partners to elicit gene-specific responses . It binds to DNA sequences containing a GGAA/T core element and can be activated by phosphorylation and interactions with other transcription factors .

Binding Partners and Downstream Signaling Cascades: ETS1 can form homodimers or heterodimers with other ETS family members, enhancing its DNA-binding affinity and specificity . It is also involved in downstream signaling cascades, such as the MAPK pathway, which phosphorylates and activates ETS1 .

Regulatory Mechanisms

Transcriptional Regulation: ETS1 expression is regulated by various signaling pathways, including the EGFR signaling pathway . Sequential activation of ETS proteins provides a sustained transcriptional response to extracellular signals .

Post-Translational Modifications: ETS1 activity is modulated by post-translational modifications, such as phosphorylation. Phosphorylation by MAPK and other kinases can either activate or inhibit ETS1’s DNA-binding ability .

Applications

Biomedical Research: ETS1 is widely studied in the context of cancer biology, immune regulation, and developmental biology . It serves as a model for understanding transcription factor networks and gene regulation .

Diagnostic Tools: Antibodies targeting ETS1 are used in various scientific applications, including Western Blot, Immunohistochemistry, and ELISA . These tools help in detecting ETS1 expression in different tissues and disease states.

Therapeutic Strategies: ETS1 is a potential therapeutic target for cancer treatment, as its dysregulation is associated with tumor progression and metastasis . Inhibitors targeting ETS1 activity are being explored as potential cancer therapies .

Role in the Life Cycle

Development: ETS1 is involved in the differentiation and development of various cell types, including immune cells and endothelial cells . It plays a critical role in embryonic development and tissue morphogenesis .

Aging and Disease: Dysregulation of ETS1 is linked to various diseases, including cancer, autoimmune disorders, and inflammatory diseases . Its role in immune cell function makes it a key player in age-related immune decline and chronic inflammation .

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