Recombinant Proteins

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SIRPG Human, Sf9

Signal-Regulatory Protein Gamma Human Recombinant, Sf9

Recombinant human SIRPG, produced in Sf9 insect cells using a baculovirus expression system, is a single polypeptide chain with glycosylation. It comprises 574 amino acids, spanning from residue 29 to 360a.a., and exhibits a molecular weight of 64.0 kDa. The protein is engineered with a 239 amino acid hIgG-His tag fused at its C-terminus. Purification is achieved through proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT27443
Source

Sf9, Baculovirus cells.

Appearance
A clear, colorless solution that has been sterilized by filtration.

SIRPG Human

Signal-Regulatory Protein Gamma Human Recombinant

Recombinant human SIRPG, expressed in E. coli, is available as a non-glycosylated polypeptide chain comprising 355 amino acids (specifically, residues 29-360). This protein variant has a molecular weight of 39.1 kDa. For purification and detection purposes, a 23 amino acid His-tag is fused to the N-terminus of the SIRPG protein. Purification is carried out using proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT27537
Source
Escherichia Coli.
Appearance
The product is a clear, colorless solution that has been sterilized by filtration.

SIRPA Human

Signal-Regulatory Protein Alpha Human Recombinant

Recombinant human SIRPA protein was produced in E. coli. It is a single, non-glycosylated polypeptide chain containing 370 amino acids (residues 27-373) with a molecular mass of 40.4 kDa. The protein includes a 23 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT27087
Source
Escherichia Coli.
Appearance
Clear, colorless, and sterile-filtered solution.

SIRPA Human, HEK

Signal-Regulatory Protein Alpha Human Recombinant, HEK

Recombinant Human SIRPA, expressed in HEK293 cells, is a single, glycosylated polypeptide chain encompassing amino acids 27-373. With a molecular weight of 39kDa, this protein comprises 356 amino acids.
A 6-amino acid His-tag is fused to the C-terminus of SIRPA, facilitating purification via proprietary chromatographic methods.

Shipped with Ice Packs
Cat. No.
BT27198
Source

HEK293 Cells.

Appearance
A clear, sterile-filtered solution.

SIRPA Rat

Signal-Regulatory Protein Alpha Rat Recombinant

Recombinant Rat SIRPA, produced in Sf9 Baculovirus cells, is a single glycosylated polypeptide chain containing 350 amino acids (32-373). It has a molecular mass of 38.5 kDa and migrates at 57-70 kDa on SDS-PAGE under reducing conditions. The protein is fused to an 8 amino acid His-tag at the C-terminus and purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT27270
Source
Sf9, Baculovirus cells.
Appearance
Clear, sterile-filtered solution.

SIRPB1 Human

Recombinant Human Signal Regulatory Protein Beta 1

Produced in Sf9 Baculovirus cells, SIRPB1 is a single, glycosylated polypeptide chain (amino acids 30-371) with a 6-amino acid His Tag fused to the C-terminus. It comprises 348 amino acids, resulting in a molecular mass of 38.0 kDa. On SDS-PAGE under reducing conditions, SIRPB1 exhibits multiple bands between 40-57 kDa. It is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT27355
Source
Sf9, Baculovirus cells.
Appearance
Sterile Filtered colorless solution.
Definition and Classification

Signal-Regulatory Proteins (SIRPs) are a family of transmembrane glycoproteins involved in immunological signaling, primarily expressed by myeloid cells . The SIRP family includes several members such as SIRPα, SIRPβ1, SIRPβ2, SIRPδ, and SIRPγ . These proteins are characterized by their extracellular immunoglobulin-like domains and their role in cell-cell communication .

Biological Properties

Key Biological Properties: SIRPs are involved in various immunological processes, including the regulation of phagocytosis and immune cell signaling .

Expression Patterns: SIRPs are predominantly expressed in myeloid cells, including macrophages, dendritic cells, and neutrophils .

Tissue Distribution: SIRPs are found in various tissues, with high expression levels in the immune system, particularly in the spleen, lymph nodes, and bone marrow .

Biological Functions

Primary Biological Functions: SIRPs play a crucial role in the regulation of immune responses, including the inhibition of phagocytosis and the modulation of cytokine production .

Role in Immune Responses: SIRPα, for example, interacts with CD47 to deliver a “don’t eat me” signal, preventing the phagocytosis of healthy cells by macrophages .

Pathogen Recognition: SIRPs are also involved in the recognition and clearance of pathogens by modulating the activity of immune cells .

Modes of Action

Mechanisms with Other Molecules and Cells: SIRPs interact with various ligands, including CD47, to mediate their effects .

Binding Partners: The primary binding partner for SIRPα is CD47, which is ubiquitously expressed on cell surfaces .

Downstream Signaling Cascades: Upon binding to CD47, SIRPα recruits and activates protein tyrosine phosphatases such as SHP-1 and SHP-2, leading to the inhibition of phagocytosis and modulation of other immune responses .

Regulatory Mechanisms

Control of Expression and Activity: The expression and activity of SIRPs are regulated at multiple levels, including transcriptional and post-translational modifications .

Transcriptional Regulation: Various transcription factors and signaling pathways regulate the expression of SIRP genes .

Post-Translational Modifications: SIRPs undergo post-translational modifications such as phosphorylation, which can modulate their activity and interactions with other proteins .

Applications

Biomedical Research: SIRPs are studied for their role in immune regulation and potential therapeutic targets .

Diagnostic Tools: SIRP expression levels can serve as biomarkers for certain diseases, including cancers and autoimmune disorders .

Therapeutic Strategies: Targeting the CD47-SIRPα interaction is being explored as a therapeutic strategy for cancer, as blocking this interaction can enhance the phagocytosis of tumor cells by macrophages .

Role in the Life Cycle

Development: SIRPs are involved in the development and differentiation of immune cells .

Aging: Changes in SIRP expression and function have been associated with aging and age-related diseases .

Disease: Dysregulation of SIRP signaling has been implicated in various diseases, including cancer, autoimmune disorders, and infectious diseases .

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