Recombinant Proteins
Product List
Collagen-IV Bovine
Bovine Collagen-IV
Bovine Collagen-IV is a naturally occurring protein extracted from bovine placenta. This collagen type is purified using proprietary chromatographic methods.
Bovine placenta.
This product appears as a white lyophilized (freeze-dried) powder that has been filtered.
Collagen-V Bovine
Bovine Collagen-V
Collagen-V, of bovine origin, is a naturally occurring protein extracted from bovine placenta. The purification process of Collagen-V involves proprietary chromatographic techniques.
Bovine placenta.
Collagen-V presents as a white, lyophilized (freeze-dried) powder that has been filtered.
COL4A3 Human
Collagen Type IV Alpha 3 Human Recombinant
This product represents the human α3 chain of collagen IV, which is synonymous with the antigen termed 'glomerular basal membrane antigen' (GBM). It serves as a recombinant antigen in both solid-phase (ELISA) and fluid-phase diagnostic assays. Its calculated molecular weight is 53kDa and its calculated isoelectric point is pH 8.9. The product consists of cDNA encoding a minicollagen version of the human collagen IV α3 chain, fused to a hexa-histidine purification tag. The designation 'minicollagen' signifies the removal of a significant portion of the triple-helical collagenous region, located between the N-terminal 7S domain and the C-terminal noncollagenous NC1 domain. This removal is essential for the recombinant production of this antigen.
The product appears as a clear solution that has undergone sterile filtration.
COL4A3 Human, Biotin
Collagen Type IV Alpha 3 Human Recombinant, Biotinylated
This product represents the human alpha3 chain of collagen IV, also known as the glomerular basement membrane antigen (GBM). Its estimated molecular weight is 48kDa, and its calculated isoelectric point is pH 8.9. This product is a cDNA sequence that codes for a shortened version of the human collagen IV alpha3 chain. This 'minicollagen' version has most of its triple-helical collagenous region removed (this region lies between the N-terminal 7S domain and the C-terminal noncollagenous NC1 domain) to facilitate recombinant production. It also includes a 6-histidine purification tag fused to the minicollagen.
CTHRC1 Human, Sf9
Collagen Triple Helix Repeat Containing 1 Human Recombinant, Sf9
Sf9, Baculovirus cells.
Introduction
Collagen is the most abundant protein in the human body, constituting about 25-35% of the total protein content. It is a key structural protein found in the extracellular matrix of various connective tissues, including skin, bones, tendons, ligaments, and cartilage . Collagen molecules are composed of three polypeptide chains that form a triple helix structure . There are at least 28 different types of collagen, classified based on their structure and function . The most common types include:
Collagen exhibits several key biological properties:
- Expression Patterns: Collagen is synthesized primarily by fibroblasts and other specialized cells such as osteoblasts and chondrocytes .
- Tissue Distribution: It is widely distributed across various tissues, providing structural integrity and mechanical strength. For instance, Type I collagen is abundant in skin and bones, while Type II is mainly found in cartilage .
- Hierarchical Structure: Collagen fibers are organized into fibrils, fibers, and bundles, each contributing to the unique mechanical properties of different tissues .
Collagen plays several crucial roles in the body:
- Structural Support: It provides tensile strength and structural integrity to tissues .
- Immune Responses: Collagen can influence immune responses by interacting with immune cells and modulating inflammation .
- Pathogen Recognition: Certain collagen types can bind to pathogens, aiding in their recognition and clearance by the immune system .
Collagen interacts with various molecules and cells through several mechanisms:
- Binding Partners: Collagen binds to cell surface receptors such as integrins and discoidin domain receptors (DDRs), initiating signaling cascades .
- Downstream Signaling: These interactions trigger downstream signaling pathways that regulate cell proliferation, differentiation, and migration .
- Enzymatic Breakdown: Enzymes like collagenases break down collagen, which is crucial for tissue remodeling and repair .
The expression and activity of collagen are tightly regulated:
- Transcriptional Regulation: Genes encoding collagen are regulated by transcription factors such as TGF-β and BMP1 .
- Post-Translational Modifications: Collagen undergoes several post-translational modifications, including hydroxylation and glycosylation, which are essential for its stability and function .
- Epigenetic Regulation: DNA methylation and histone modifications also play a role in regulating collagen gene expression .
Collagen has numerous applications in biomedical research and clinical practice:
- Biomedical Research: Collagen-based scaffolds are used in tissue engineering and regenerative medicine .
- Diagnostic Tools: Collagen biomarkers are used in diagnosing and monitoring diseases such as fibrosis and osteoarthritis .
- Therapeutic Strategies: Collagen is used in wound healing, drug delivery systems, and as a component of medical devices .
Collagen plays a vital role throughout the human life cycle:
- Development: During development, collagen provides the necessary framework for tissue formation and organ development .
- Aging: Collagen production decreases with age, leading to wrinkles, reduced skin elasticity, and weakened connective tissues .
- Disease: Alterations in collagen structure and function are associated with various diseases, including fibrosis, osteoarthritis, and certain cancers .
