SLAMF6 Human, sf9
Description
Functional Properties
Immune Modulation:
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Promotes IFNγ production in NK cells and modulates Th17 differentiation .
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In T cells, SLAMF6 engagement enhances IL-17 secretion via RORC recruitment .
Signaling Pathways:
Disease Relevance:
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Defective SLAMF6 signaling in SLE patients reduces Th1/IL-2 production .
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Inhibits CD8+ T cell antitumor activity; deletion enhances tumor clearance .
Comparative Analysis of Recombinant SLAMF6 Variants
Research Applications
Immune Checkpoint Studies:
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SLAMF6Δ17-65 isoform exhibits co-stimulatory activity in T cells, contrasting canonical inhibitory isoforms .
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CRISPR-edited Jurkat T cells lacking SLAMF6 show enhanced antitumor responses .
Therapeutic Development:
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Antisense oligonucleotides targeting SLAMF6 splice variants improve adoptive T cell therapy in melanoma models .
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Combined SLAMF6/LAG-3 blockade synergistically enhances CD8+ T cell cytotoxicity .
Autoimmunity Mechanisms:
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SLAMF6 deficiency disrupts B cell tolerance, promoting autoantibody production .
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Murine studies link SLAMF6 to defective Th2 responses and neutrophil-mediated inflammation .
Technical Considerations
Bioactivity Validation:
Limitations:
Product Specs
Product Science Overview
Signaling Lymphocytic Activation Molecule Family member 6 (SLAMF6), also known as CD352, is a cell surface receptor that plays a crucial role in the regulation of immune responses. It is part of the SLAM family of receptors, which are involved in the modulation of T cell and B cell functions. SLAMF6 is expressed on various immune cells, including T cells, B cells, natural killer (NK) cells, and dendritic cells.
SLAMF6 is a type I transmembrane protein characterized by an extracellular domain, a transmembrane domain, and a cytoplasmic tail. The extracellular domain contains immunoglobulin-like domains that are responsible for homophilic interactions, meaning SLAMF6 can bind to itself on adjacent cells. This interaction is essential for the modulation of immune cell functions.
The cytoplasmic tail of SLAMF6 contains immunoreceptor tyrosine-based switch motifs (ITSMs), which are critical for signal transduction. Upon ligand binding, these ITSMs recruit adaptor proteins such as SAP (SLAM-associated protein), which in turn activate downstream signaling pathways. These pathways are involved in the regulation of cytokine production, cell proliferation, and cytotoxic activity.
SLAMF6 plays a significant role in the regulation of T cell and NK cell functions. It has been shown to enhance the cytotoxic activity of NK cells and promote the production of interferon-gamma (IFN-γ) by T cells. Additionally, SLAMF6 is involved in the formation of the immunological synapse, a specialized junction between T cells and antigen-presenting cells that is crucial for effective immune responses.
In the context of cancer, SLAMF6 has been identified as a regulator of exhausted CD8+ T cells. Exhausted T cells are characterized by impaired effector functions and are often found in the tumor microenvironment. Targeting SLAMF6 has been shown to restore the functionality of these exhausted T cells, making it a potential therapeutic target for cancer immunotherapy .
Recombinant SLAMF6 (Human, sf9) refers to the SLAMF6 protein that has been produced using the baculovirus expression system in Sf9 insect cells. This system is widely used for the production of recombinant proteins due to its ability to produce high yields of properly folded and functional proteins. The recombinant SLAMF6 produced in this system retains its biological activity and can be used for various research applications, including functional assays and structural studies.
Recombinant SLAMF6 is used in a variety of research applications to study its role in immune regulation and its potential as a therapeutic target. Some of the key applications include:
- Functional assays: Investigating the effects of SLAMF6 on immune cell functions, such as cytokine production, cell proliferation, and cytotoxic activity.
- Structural studies: Determining the three-dimensional structure of SLAMF6 to understand its interactions with other proteins and its role in signal transduction.
- Cancer research: Exploring the potential of SLAMF6 as a target for cancer immunotherapy by studying its effects on exhausted T cells and tumor progression.
