SNRPE Human

Small Nuclear Ribonucleoprotein Polypeptide E Human Recombinant
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Cat. No.
BT1576
Source
Escherichia Coli.
Synonyms
Small nuclear ribonucleoprotein E, snRNP-E, Sm protein E, Sm-E, SmE, SNRPE, B-raf.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Role in Cancer Biology

SNRPE dysregulation is implicated in multiple cancers, influencing proliferation and therapy resistance:

Prostate Cancer

  • Overexpressed in high-grade tumors (Gleason score 8–10), driving androgen receptor (AR) signaling .

    • Key findings:

      • siRNA-mediated SNRPE knockdown reduced AR mRNA by ≥80%, suppressing cell viability .

      • Exogenous SNRPE expression promoted tumor growth via AR pathway activation .

Breast Cancer

  • Targeting SNRPE triggers pyroptosis (inflammatory cell death) and enhances NK cell-mediated antitumor immunity .

Pan-Cancer Associations

Data from The Human Protein Atlas and Cancer Genetics Web highlight SNRPE’s expression in :

Cancer TypePubMed PublicationsClinical Relevance
Liver2Correlated with advanced-stage tumors
Acute Lymphocytic Leukemia1Potential diagnostic marker
Bladder1Overexpression in metastatic variants

Genetic Disorders

Mutations in SNRPE are linked to hypotrichosis simplex type 11 (HYPT11), an autosomal-dominant hair loss disorder :

  • Pathogenic variants:

    • c.133G>A (p.Gly45Ser): Disrupts SNRPE solubility, impairing spliceosome integration .

    • c.1A>G (p.Met1?): Truncates protein, reducing functional snRNP assembly .

  • Mechanism: Mutant SNRPE proteins destabilize hair follicle mRNA splicing, leading to follicular miniaturization .

Targeting SNRPE in Oncology

  • Prostate cancer: SNRPE inhibitors could suppress AR-driven proliferation, offering a strategy for castration-resistant cases .

  • Breast cancer: SNRPE knockdown synergizes with immunotherapies by inducing pyroptosis and activating NK cells .

Challenges

  • Ubiquitous expression of SNRPE in normal tissues raises toxicity concerns for systemic therapies .

Research Frontiers

Recent studies highlight novel regulatory mechanisms:

  • Splice site selection: LUC7 proteins collaborate with SNRPE to regulate spliceosome targeting, influencing ~50% of human introns .

  • Evolutionary conservation: SNRPE’s role in splicing complexity may explain its retention across eukaryotes, despite redundancy in spliceosomal components .

Product Specs

Introduction
SNRPE plays a crucial role in the assembly of UsnRNPs (U small nuclear ribonucleoproteins) within the cytoplasm. It achieves this by binding to a conserved Sm site on UsnRNA, leading to the formation of a stable snRNP core complex. As a core protein of UsnRNP, SNRPE interacts with all members of the U family of snRNAs, including U1 through U6. Additionally, SNRPE interacts with other proteins such as DDX20 and Small nuclear ribonucleoprotein polypeptide F.
Description
This product consists of the human recombinant SNRPE protein, fused with a 20 amino acid His tag at its N-terminus. It is produced in E.Coli and is a single, non-glycosylated polypeptide chain. The protein comprises 112 amino acids (specifically, amino acids 1 to 92 of the SNRPE sequence) and has a molecular weight of 12.9kDa. Purification of SNRPE is achieved using proprietary chromatographic techniques.
Physical Appearance
The product is a sterile, colorless solution.
Formulation
The SNRPE solution is provided at a concentration of 0.25 mg/ml. It is formulated in a buffer consisting of 20mM Tris-HCl (pH 8.0), 0.2M NaCl, 5mM DTT, 1mM EDTA, and 30% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For longer storage, it is recommended to freeze the product at -20°C. To further enhance long-term stability, consider adding a carrier protein (0.1% HSA or BSA). It is important to avoid repeated freezing and thawing of the product.
Purity
The purity of the SNRPE protein is greater than 95%, as determined by SDS-PAGE analysis.
Synonyms
Small nuclear ribonucleoprotein E, snRNP-E, Sm protein E, Sm-E, SmE, SNRPE, B-raf.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MAYRGQGQKV QKVMVQPINL IFRYLQNRSR IQVWLYEQVN MRIEGCIIGF DEYMNLVLDD AEEIHSKTKS RKQLGRIMLK GDNITLLQSV SN.

Product Science Overview

Gene and Protein Structure

The SNRPE gene is located on chromosome 1 and encodes the SNRPE protein . This protein is also known by several aliases, including Sm-E, Sm Protein E, and SnRNP-E . The SNRPE protein is a small, highly conserved protein that plays a critical role in the assembly and function of snRNPs.

Function and Mechanism

SNRPE is involved in several key processes within the cell:

  1. Pre-mRNA Splicing: As a core component of the spliceosomal U1, U2, U4, and U5 snRNPs, SNRPE is integral to the splicing of pre-mRNA . This process involves the removal of non-coding sequences (introns) and the joining of coding sequences (exons) to form mature mRNA.
  2. Histone 3’ End Processing: SNRPE is also part of the U7 snRNP, which is involved in the 3’ end processing of histone transcripts . Histones are proteins that package and order DNA into structural units called nucleosomes.
  3. Minor Spliceosome: SNRPE is a component of the minor spliceosome, which is responsible for the splicing of U12-type introns in pre-mRNAs .
Clinical Significance

Mutations in the SNRPE gene have been associated with several disorders:

  • Systemic Lupus Erythematosus (SLE): SNRPE is one of the targets in the autoimmune disease SLE, where patients produce autoantibodies against snRNPs .
  • Hypotrichosis 11: This condition is characterized by sparse hair growth and has been linked to mutations in the SNRPE gene .
Research and Applications

Recombinant SNRPE protein is used in various research applications, including studies on RNA splicing, autoimmune diseases, and gene expression regulation. The availability of human recombinant SNRPE allows researchers to investigate its function and interactions in a controlled environment, providing insights into its role in cellular processes and disease mechanisms.

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