TAF10 Human
Description
Functional Roles in Transcription
TFIID Complex Dynamics
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Core Function: TAF10 is integral to the TFIID basal transcription factor complex, which binds promoters (with/without TATA boxes) to assemble the pre-initiation complex (PIC) .
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Subunit Interactions: Forms stable interactions with TBP, TAF8, TAF9, and TAF13, enabling TFIID’s structural asymmetry and promoter selectivity .
Regulatory Complexes
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SAGA/STAGA/PCAF: TAF10 is shared between TFIID and histone acetyltransferase complexes (e.g., SAGA), linking promoter recognition to chromatin remodeling .
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Cytoplasmic Assembly: Participates in a cytoplasmic TAF2–TAF8–TAF10 subcomplex, which primes nuclear import and holo-TFIID assembly .
Key Functional Partners
Biological and Clinical Implications
Developmental Roles
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Embryogenesis: TAF10 knockout in mice causes embryonic lethality by E5.5 due to disrupted TFIID stability and cell cycle arrest .
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Erythropoiesis: TAF10 ablation blocks erythroid differentiation, deregulating GATA1 and its targets (e.g., hemoglobin genes) .
Disease Associations
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Neurological Disorders: Mutations in TAF2 (a TAF10 interactor) correlate with neurodevelopmental defects .
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Cancer: TAF10-dependent cyclin E regulation suggests roles in cell proliferation .
Research Tools
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Antibodies: Polyclonal antibodies against recombinant TAF10 enable immunoprecipitation and localization studies .
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Cellular Models: Conditional TAF10 knockout in F9 embryonal carcinoma cells reveals TFIID’s role in pluripotency .
Research Applications and Directions
Ongoing Studies
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Complex Stoichiometry: Mass spectrometry reveals dynamic TFIID/SAGA composition during erythroid differentiation .
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Gene-Specific Recruitment: ChIP-seq data show TAF10 enrichment at the GATA1 locus in fetal erythroid cells .
Therapeutic Potential
Product Specs
Product Science Overview
The initiation of transcription by RNA polymerase II requires the coordinated activities of more than 70 polypeptides. TFIID, which includes TAF10, is essential for this process. TFIID binds to the core promoter region of genes to position RNA polymerase II correctly, serving as a scaffold for the assembly of the transcription complex and acting as a channel for regulatory signals .
TAF10, specifically, is involved in several critical functions:
- Basal Transcription: TAF10 participates in the basic transcriptional machinery, ensuring that the transcription process can begin.
- Coactivator Functions: It serves as a coactivator, helping to enhance the transcriptional activity of specific genes.
- Promoter Recognition: TAF10 aids in recognizing and binding to promoter regions of genes, facilitating the assembly of the transcription complex.
- Modification of General Transcription Factors (GTFs): It helps modify GTFs to facilitate the assembly and initiation of the transcription complex .
TAF10 interacts with several other proteins and factors within the cell:
- TAF9: Another subunit of TFIID, which works closely with TAF10.
- SPT3 Homolog: A transcription initiation protein that interacts with TAF10.
- TAF13: Another TFIID subunit that interacts with TAF10.
- TATA Binding Protein (TBP): The core component of TFIID that binds to the TATA box in the promoter region .
Studies have shown that TAF10 is required for transcriptional activation by the estrogen receptor, progression through the cell cycle, and certain cellular differentiation programs .
