TGFB3 (24-412 a.a.) Human
Description
Molecular Structure and Isoforms
TGFB3 (24-412 a.a.) corresponds to the proprotein region after cleavage of the 20 a.a. signal peptide. It includes:
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Latency-associated peptide (LAP): 24-220 a.a., regulates TGFB3 activation .
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Mature TGFB3: 301-412 a.a., the bioactive domain responsible for receptor binding .
Biological Functions
TGFB3 regulates:
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Embryogenesis: Critical for palatogenesis, pulmonary development, and cardiac morphogenesis .
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Wound healing: Accelerates repair by modulating cell adhesion and extracellular matrix synthesis .
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Immune regulation: Inhibits IL-4-dependent T-cell proliferation (ED50: 40 ng/ml) .
Table 2: Functional Activity Data
Recombinant Production
TGFB3 (24-412 a.a.) is produced in multiple systems with varying characteristics:
Table 3: Recombinant Variants
Clinical and Research Relevance
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Genetic mutations: Heterozygous TGFB3 mutations (e.g., c.754+2T>C) cause syndromic aortic aneurysms, mitral valve disease, and skeletal abnormalities .
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Therapeutic potential:
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Signaling paradox: Mutations upregulate canonical (Smad2/3) and noncanonical (ERK, JNK) TGF-β pathways, contributing to vascular pathology .
Product Specs
Product Science Overview
The human recombinant TGF-β3 (24-412 a.a.) is produced in E. coli and consists of a single, non-glycosylated polypeptide chain containing 412 amino acids, with a molecular mass of approximately 47.2 kDa . This recombinant protein is fused to a 23 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques .
TGF-β3 plays a crucial role in various biological processes, including:
- Cell Differentiation: It regulates the differentiation of various cell types, contributing to tissue development and repair .
- Embryogenesis: TGF-β3 is essential for proper embryonic development, particularly in the formation of the palate and lungs .
- Wound Healing: It orchestrates the movements of epidermal and dermal cells during the wound healing process .
- Extracellular Matrix Formation: TGF-β3 regulates molecules involved in cellular adhesion and extracellular matrix (ECM) formation .
Mutations in the TGFB3 gene are associated with several medical conditions, including:
Recombinant TGF-β3 is widely used in research to study its role in various cellular processes and its potential therapeutic applications. Despite its promising effects in preclinical studies, human recombinant TGF-β3 (avotermin) failed in Phase III clinical trials for wound healing .
In summary, TGF-β3 is a vital cytokine with significant roles in cell differentiation, embryogenesis, and wound healing. Its recombinant form, produced in E. coli, is a valuable tool for scientific research and potential therapeutic applications.
