TNF B Human, Sf9
Description
Biological Activity and Functional Insights
TNF-B Human, Sf9 demonstrates cytotoxic activity in murine L-929 fibrosarcoma cells, with an ED50 ≤ 1 ng/mL in the presence of actinomycin D . Key functional roles include:
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Immune Regulation: Mediates inflammatory and antiviral responses via TNFR1/2 signaling .
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Lymphoid Development: Critical for secondary lymphoid organ formation during embryogenesis .
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Apoptosis Induction: Triggers cell death in target cells under specific inflammatory conditions .
Unlike TNF-α (produced by macrophages), TNF-β is primarily secreted by activated lymphocytes and exists as a homotrimer for bioactivity .
Comparative Analysis with Other TNF Forms
| Feature | TNF-B (LT-α) | TNF-α |
|---|---|---|
| Primary Source | Lymphocytes (T/B cells) | Macrophages, dendritic cells |
| Receptor Binding | TNFR1 and TNFR2 | Primarily TNFR1 |
| Glycosylation Status | Glycosylated (Sf9 system) | Non-glycosylated (E. coli systems) |
| Therapeutic Target | Autoimmune disorders, cancer | Rheumatoid arthritis, IBD |
The Sf9-derived TNF-B exhibits higher solubility and avoids inclusion body formation common in E. coli-produced cytokines .
Research Applications and Findings
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Cytotoxicity Assays: Used to study TNF-β-mediated cell death pathways in cancer models .
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Inflammation Studies: Evaluates cross-talk between TNFR1/2 and MAPK/NF-κB pathways .
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Therapeutic Development: Silkworm-BEVS systems show scalable TNF-β production with retained bioactivity, offering cost-effective alternatives .
Recent advancements in Baculovirus systems (e.g., ExpiSf) report 4-fold higher TNF-α yields compared to traditional Sf9 workflows, suggesting potential optimizations for TNF-B production .
Product Specs
Product Science Overview
The human recombinant TNF-β produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 180 amino acids (35-205a.a.) and has a molecular mass of approximately 19.7 kDa . The recombinant protein is fused with a 6 amino acids His tag at the C-terminus and is purified using proprietary chromatographic techniques .
TNF-β mediates a wide range of biological activities, including:
- Inflammatory Responses: TNF-β is highly inducible and secreted by lymphocytes. It forms homotrimers and heterotrimers with lymphotoxin-beta, anchoring it to the cell surface .
- Immunostimulatory and Antiviral Responses: It plays a significant role in stimulating the immune system and combating viral infections .
- Apoptosis: TNF-β is involved in the process of programmed cell death, which is essential for maintaining cellular homeostasis and eliminating cancerous cells .
- Development of Secondary Lymphoid Organs: TNF-β is crucial for the formation of secondary lymphoid organs such as lymph nodes and Peyer’s patches during development .
The recombinant TNF-β protein is used extensively in laboratory research to study its effects on various cell types and its role in different biological processes. It is typically stored at 4°C if used within 2-4 weeks or frozen at -20°C for longer periods. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA) to avoid multiple freeze-thaw cycles .
The purity of the recombinant TNF-β protein is greater than 90.0% as determined by SDS-PAGE . Its biological activity is measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The effective dose (ED50) for this effect is ≤ 1 ng/ml .
