TROVE2 His Human

TROVE Domain Family Member 2 His Tag Human Recombinant
Shipped with Ice Packs
In Stock
Cat. No.
BT19483
Source
Escherichia Coli.
Synonyms
60 kDa SS-A/Ro ribonucleoprotein, 60 kDa ribonucleoprotein Ro, RoRNP, 60 kDa Ro protein, Ro 60 kDa autoantigen, TROVE domain family member 2, Sjoegren syndrome type A antigen, SS-A, Sjoegren syndrome antigen A2, TROVE2, RO60, SSA2, RO-60.
Purity

Greater than 95% as determined by SDS-PAGE.

Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Quick Inquiry

Description

Production and Biochemical Properties

TROVE2 His Human is expressed in Escherichia coli and purified using chromatographic methods. Variants differ in buffer composition and tag placement:

  • N-terminal His-tagged versions (e.g., PRO-110, A113426): Optimized for ELISA and biotinylation .

  • C-terminal His-tagged versions (e.g., PRO-329): Stabilized in urea-based buffers for denaturing conditions .

TROVE2 His Human serves as a critical antigen in autoimmune disease studies and cancer research:

Immunological Assays

  • ELISA: Coating concentration: 0.2–0.5 µg/ml. Detects anti-TROVE2 autoantibodies in systemic lupus erythematosus (SLE) and Sjögren’s syndrome (SS) patient sera .

  • Western Blot: Validated with SLE sera or anti-His-tag antibodies .

Cancer Research Findings

A 2024 study identified TROVE2 as a driver of hepatocellular carcinoma (HCC) metastasis via the HPSE/GSK-3β/Snail signaling axis :

ProcessTROVE2 Overexpression EffectTROVE2 Knockdown Effect
Cell Invasion↑↑ (Transwell assay)↓↓
EMT Markers↑ N-cadherin, vimentin; ↓ E-cadherin↓ N-cadherin, vimentin; ↑ E-cadherin
GSK-3β Phosphorylation↑ p-GSK-3β (inhibition of GSK-3β activity)↓ p-GSK-3β

Autoimmune Diseases

TROVE2 is a primary autoantigen in SLE and SS, with sera from patients targeting its RNA-binding domains. The His-tagged protein is used to:

  • Diagnose autoimmune conditions via serological testing .

  • Study epitope-specific immune responses .

Oncological Implications

In HCC, TROVE2 upregulation correlates with poor prognosis. Mechanistically, it binds heparanase (HPSE), promoting epithelial-to-mesenchymal transition (EMT) through:

  1. Snail stabilization: Inhibition of GSK-3β activity prolongs Snail degradation resistance .

  2. Wnt/β-catenin signaling: Enhanced p-GSK-3β levels suppress β-catenin phosphorylation, sustaining EMT .

Product Specs

Introduction
Ro 60 kDa autoantigen, also known as TROVE2, is an RNA-binding protein that interacts with small cytoplasmic RNA molecules called Y RNAs. SSA2, another name for this protein, may play a role in stabilizing these RNAs and preventing their degradation. Individuals with Systemic Lupus Erythematosus (SLE) often exhibit antibodies that target the normal SSA2 protein, indicating an autoimmune response.
Description
TROVE2 His is a recombinant protein with a molecular weight of 64,000 Daltons (pH 8.3-8.6, depending on the prediction program used). This protein is engineered to include a hexa-histidine purification tag.
Formulation
TROVE2 is supplied in a buffer solution containing 20mM HEPES (pH 8.0), 150mM NaCl, 1mM DTT, and 10% glycerol.
Immunological Functions
1. Binds to human auto-antibodies of the IgG class. 2. Suitable for use in various immunoassays, including standard ELISA (with checkerboard analysis of positive and negative samples), line assays, and immunodot tests utilizing positive and negative samples.
Coating Concentration
The recommended coating concentration for TROVE2 is 0.2-0.5 µg/ml. This range may vary depending on the specific ELISA plate and coating buffer used. TROVE2 is also suitable for labeling functional groups.
Purity
The purity of TROVE2 is greater than 95%, as determined by SDS-PAGE analysis.
Stability
For short-term storage (up to 2-4 weeks), TROVE2 can be stored at 4°C. For longer storage, freeze the protein at -20°C. To maintain protein integrity, avoid repeated freeze-thaw cycles.
Synonyms
60 kDa SS-A/Ro ribonucleoprotein, 60 kDa ribonucleoprotein Ro, RoRNP, 60 kDa Ro protein, Ro 60 kDa autoantigen, TROVE domain family member 2, Sjoegren syndrome type A antigen, SS-A, Sjoegren syndrome antigen A2, TROVE2, RO60, SSA2, RO-60.
Source
Escherichia Coli.

Product Science Overview

Structure and Production

The Human Recombinant TROVE2 is produced in Escherichia coli (E. coli) and is a single, non-glycosylated polypeptide chain with a molecular mass of approximately 60 kDa . The recombinant protein is fused with a 6 amino acid His-Tag at the C-terminus, which facilitates its purification through proprietary chromatographic techniques .

Function and Importance

TROVE2, or Ro 60 kDa autoantigen, binds to Y RNAs and may stabilize these RNAs from degradation . This stabilization is crucial for the proper functioning of cellular processes. The protein is also known to be involved in autoimmune responses, as sera from patients with Systemic Lupus Erythematosus (SLE) often contain antibodies that react with the normal cellular SSA2 protein .

Applications in Research

The recombinant TROVE2 protein is widely used in laboratory research, particularly in studies related to autoimmune diseases like SLE and Sjögren’s syndrome . It is also used in various biochemical assays to understand its interaction with RNA molecules and its role in cellular processes .

Storage and Stability

The recombinant TROVE2 protein, although stable at 4°C for three weeks, should be stored below -18°C to prevent degradation . It is essential to avoid freeze-thaw cycles to maintain its stability and functionality .

Safety and Usage

Prospec’s recombinant TROVE2 protein is intended for laboratory research use only and is not suitable for use as drugs, agricultural or pesticidal products, food additives, or household chemicals . Researchers must handle it with care and follow appropriate safety guidelines.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

TROVE2 Human, His Biotin
TROVE Domain Family Member 2 Human Recombinant, His Tag Biotinylated
Cat. No.
BT19711
Source
Escherichia Coli.
TROVE2 Bovine
TROVE Domain Family, Member 2, Bovine
Cat. No.
BT19282
Source
Bovine tissues.
TROVE2 Human
TROVE Domain Family Member 2 Human Recombinant
Cat. No.
BT19651
Source
Escherichia Coli.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.