CD44 Antibody, FITC
Description
Key Features of CD44 Antibody, FITC
Target: CD44 antigen (UniProt IDs: P16070, P15379)
Conjugate: Fluorescein isothiocyanate (FITC) with excitation/emission maxima at 495/524 nm .
Host Species: Mouse, rat, or humanized clones .
Reactivity: Human, mouse, cynomolgus monkey (species-specific clones available) .
Mechanisms of Action
CD44 Antibody, FITC binds to extracellular domains of CD44, blocking ligand interactions (e.g., hyaluronic acid) and modulating downstream signaling pathways. Key findings include:
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Anti-inflammatory effects: Anti-CD44 (IM7) disrupts leukocyte rolling and adhesion in synovial vessels, reducing neutrophil infiltration in arthritis models .
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Cancer inhibition: Clone H4C4 targets CD44s isoforms on pancreatic tumor-initiating cells (TICs), suppressing STAT3 signaling and Nanog expression .
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Phagocytosis modulation: IM7 inhibits Fcγ receptor-mediated phagocytosis in macrophages, independent of CD44’s native function .
Table 2: Functional Outcomes in Disease Models
Autoimmune Disease Studies
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Leukocyte tracking: Intravital microscopy in PGIA models revealed that CD44 deficiency increases leukocyte rolling by 40% but reduces firm adhesion by 60% compared to wild-type mice .
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Therapeutic targeting: Anti-CD44 (KM201) reduced granulocyte counts by >90% within 1 hour in murine models .
Cancer Research
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Stem cell targeting: FITC-65063 identifies CD44+ cancer stem cells in human PBMCs, correlating with metastatic potential .
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Radiation resistance: H4C4 antibody delayed post-radiation tumor recurrence by eliminating TICs .
Technical Performance
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Titration: Recommended dilutions range from 5 µl/10^6 cells (FITC-65063) to 200 µg per dose in vivo .
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Cross-reactivity: Clone KM201 shows specificity for mouse CD44, while B-F24 detects human isoforms .
Limitations and Considerations
Product Specs
Product Science Overview
CD44 is a cell surface glycoprotein involved in cell-cell interactions, cell adhesion, and migration. It is widely expressed on various cell types, including hematopoietic and non-hematopoietic cells. CD44 plays a crucial role in a variety of cellular functions, such as:
- Lymphocyte activation
- Recirculation and homing
- Hematopoiesis
- Tumor metastasis
The principal ligand for CD44 is hyaluronate, a common component of the extracellular matrix. CD44’s interaction with hyaluronate is essential for maintaining tissue structure and facilitating cell movement.
The Rat Anti-Mouse CD44 antibody specifically recognizes an epitope on both alloantigens and all isoforms of the CD44 glycoprotein. The standard form of CD44, known as CD44H or CD44s, lacks variable exons and is widely expressed. CD44 isoforms encoded by variable exons are expressed on epithelial cells but are present at low levels on most leukocytes .
Fluorescein isothiocyanate (FITC) is a fluorochrome used to label antibodies, making them detectable under specific wavelengths of light. The conjugation of FITC to the Rat Anti-Mouse CD44 antibody allows researchers to:
- Visualize CD44 expression on cells using flow cytometry or fluorescence microscopy.
- Quantify the level of CD44 expression on different cell populations.
- Sort cells based on CD44 expression levels.
The Rat Anti-Mouse CD44, FITC antibody is widely used in immunological research, particularly in studies involving:
- Flow cytometric analysis of CD44 expression on bone marrow cells.
- Investigating the role of CD44 in various physiological and pathological processes, including immune responses and cancer metastasis.
- Characterizing cell populations in hematopoietic and non-hematopoietic tissues.
