CXCL17 Rat
Description
Biological Functions
CXCL17 exhibits multifaceted roles in immune regulation and tissue homeostasis:
Expression and Localization
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Gastric Mucosa: Highly expressed in rat glandular stomach, with inducible upregulation by LPS, alcohol, and cAMP signaling .
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Mucosal Tissues: Detected in lung, trachea, and female reproductive tract .
Functional Studies
Receptor Interactions
CXCL17’s receptor remains debated. While earlier studies suggested GPR35 , recent evidence indicates a distinct receptor:
| Receptor | Activity | Source |
|---|---|---|
| GPR35 | Inactive despite structural homology; zaprinast (GPR35 agonist) shows no CXCL17 synergy. | |
| CXCR8 | Proposed receptor for CXCL17-dependent T cell recruitment. |
Applications and Future Directions
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Therapeutic Potential:
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Research Gaps:
Table 1: CXCL17 Rat Bioactivity
| Parameter | Value | Assay | Source |
|---|---|---|---|
| ED₅₀ for VEGF-A | <5 µg/mL | Murine endothelial cells | |
| Chemoattractant Potency | 2× higher than propeptide | Transwell migration assays |
Table 2: Signaling Pathways
Product Specs
Product Science Overview
VCC1 is a secreted molecule with a size and predicted 3-dimensional folding pattern similar to that of chemokines CXCL8/IL8 and CXCL14/BRAK . It is constitutively expressed in the lung and can also be detected in the trachea, stomach, and skeletal muscle . The mature Rat CXCL17 shares 82% and 71% amino acid sequence identity with mouse and human CXCL17, respectively .
VCC1 plays a significant role in the chemotaxis of quiescent peripheral blood monocytes and dendritic cells, but not LPS-activated ones . It specifically binds to these cells and induces their migration. The expression of VCC1 is increased in endothelial cells when they are induced to form tubes in vitro . This chemokine is also involved in angiogenesis, showing significantly correlated expression with VEGF in primary lung, breast, and esophageal tumors . Therefore, VCC1 is suggested to have a role in tumor angiogenesis.
Recombinant VCC1 is produced in Escherichia coli (E. coli) as a single, non-glycosylated polypeptide chain containing 98 amino acids and having a molecular mass of 11.5 kDa . The recombinant protein is purified using proprietary chromatographic techniques and is available as a sterile filtered white lyophilized (freeze-dried) powder .
Lyophilized VCC1 is stable at room temperature for three weeks but should be stored desiccated below -18°C for long-term storage . Upon reconstitution, it should be stored at 4°C for 2-7 days and for future use below -18°C. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA) to prevent freeze-thaw cycles .
