GPT Human

Glutamic-Pyruvate Transaminase Human Recombinant
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Description

GPT Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 516 amino acids (1-496) and having a molecular mass of 56.8 kDa.
GPT is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
GPT, also known as Alanine Transaminase, plays a vital role in the metabolic pathways of glucose and amino acids by facilitating the reversible conversion between alanine and 2-oxoglutarate to form pyruvate and glutamate. GPT is an important biomarker for assessing liver health and detecting liver damage in clinical settings.
Description
Recombinant Human GPT, expressed in E.coli, is a non-glycosylated polypeptide chain consisting of 516 amino acids (residues 1-496) with a molecular weight of 56.8 kDa. This protein is engineered with a 20 amino acid His-tag at the N-terminus and purified using proprietary chromatographic methods.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
The GPT is provided at a concentration of 0.5mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0), 2mM DTT, and 20% glycerol.
Stability
For short-term storage (up to 4 weeks), keep refrigerated at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein like HSA or BSA to a final concentration of 0.1% is recommended for long-term storage. Avoid repeated freeze-thaw cycles to maintain protein integrity.
Purity
Purity is determined to be greater than 90% by SDS-PAGE analysis.
Synonyms
Glutamic-pyruvate transaminase (alanine aminotransferase), GPT1, ALT1, AAT1, Glutamic-alanine transaminase 1, EC 2.6.1.2.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MASSTGDRSQ AVRHGLRAKV LTLDGMNPRV RRVEYAVRGP IVQRALELEQ ELRQGVKKPF TEVIRANIGD AQAMGQRPIT FLRQVLALCV NPDLLSSPNF PDDAKKRAER ILQACGGHSL GAYSVSSGIQ LIREDVARYI ERRDGGIPAD PNNVFLSTGA SDAIVTVLKL LVAGEGHTRT GVLIPIPQYP LYSATLAELG AVQVDYYLDE ERAWALDVAE LHRALGQARD HCRPRALCVI NPGNPTGQVQ TRECIEAVIR FAFEERLFLL ADEVYQDNVY AAGSQFHSFK KVLMEMGPPY AGQQELASFH STSKGYMGEC GFRGGYVEVV NMDAAVQQQM LKLMSVRLCP PVPGQALLDL VVSPPAPTDP SFAQFQAEKQ AVLAELAAKA KLTEQVFNEA PGISCNPVQG AMYSFPRVQL PPRAVERAQE LGLAPDMFFC LRLLEETGIC VVPGSGFGQR EGTYHFRMTI LPPLEKLRLL LEKLSRFHAK FTLEYS

Q&A

Methodological Considerations

  • How can researchers effectively measure emotional intelligence dimensions in GPT versus humans?

    Methodological approaches for measuring emotional intelligence in GPT models include:

    • Administering standardized EI tests designed for humans, with appropriate modifications

    • Creating scenarios requiring emotional recognition, understanding, and appropriate response

    • Comparing GPT self-assessment of EI capabilities against objective performance

    • Testing for contextual understanding of emotional cues across diverse scenarios

    • Examining ability to detect subtle emotional changes in longitudinal conversational contexts

    Research applying these methods found that GPT-3's emotional intelligence capacities matched those of an average human, though its self-assessments of EI didn't always align with its objective performance, showing variations similar to different human subsamples . This suggests that while GPT models can simulate aspects of emotional intelligence, they may lack the integrated self-awareness that characterizes human emotional processing.

  • What preprocessing techniques optimize GPT-human comparative studies?

    Effective data preprocessing techniques for GPT-human comparative studies include:

    • Standardizing response formats to ensure fair comparison

    • Anonymizing human responses to prevent biased evaluation

    • Controlling for demographic variables in human participant selection

    • Normalizing response lengths and linguistic complexity

    • Developing consistent coding schemes for qualitative responses

    Research highlights the importance of these preprocessing steps to ensure valid comparisons. Additionally, when analyzing dimensional representations of GPT and human judgments, careful preprocessing is essential to identify genuine similarities and differences rather than artifacts of data preparation .

  • How do researchers evaluate data augmentation effects when combining GPT and human responses?

    Methodological approaches for evaluating data augmentation effects include:

    • Creating mixed GPT-human datasets with varying proportions of each

    • Measuring performance changes as human data is systematically replaced with GPT data

    • Using representational similarity analysis (RSA) to quantify shifts in model structure

    • Comparing dimensional characteristics between pure and mixed datasets

    • Testing model generalization capabilities with different augmentation ratios

    Research applying these methods to object-similarity judgments found that augmenting human data with GPT responses drove model divergence across tested dataset sizes. Even when smaller human datasets were augmented with GPT responses to create larger training sets, the resulting models showed reduced RSA scores compared to models trained on smaller human-only datasets .

    Human Data ProportionRSA Score (Mixed GPT-Human)RSA Score (Human Only)
    0.75Lower (~10% reduction)Higher
    0.50LowerHigher
    0.25LowerHigher
    0.00 (All GPT)Lowest (~60% reduction)Highest
  • What approaches detect when GPT responses are indistinguishable from human responses?

    Methodological approaches for detecting GPT-human response indistinguishability include:

    • Blinded human evaluation studies where judges attempt to distinguish sources

    • Statistical analysis of linguistic patterns characteristic of each source

    • Dimensional comparison of response spaces using techniques like RSA

    • Tracking evolution of distinguishability across model versions and domains

    • Developing specialized metrics that capture subtle differences in response patterns

    Research demonstrates that while GPT responses may appear superficially similar to human responses, statistical analyses can reveal underlying differences. Studies using representational similarity analysis show that human judges may struggle to distinguish individual responses while statistical methods can still detect systematic differences in how concepts are represented .

  • How can researchers develop reliable benchmarks for evaluating GPT-human alignment?

    Developing reliable benchmarks requires multifaceted methodological approaches:

    • Creating diverse task batteries spanning cognitive, emotional, and social domains

    • Establishing large, representative human baseline datasets across demographics

    • Developing standardized prompting protocols to ensure consistency

    • Employing multiple evaluation metrics addressing different alignment aspects

    • Ensuring tasks reflect ecologically valid scenarios rather than artificial problems

    Research demonstrates the importance of using multiple metrics rather than single-dimension comparisons. For example, studies examining both performance-based measures and dimensional representations reveal that GPT models may show similar performance to humans while organizing conceptual information differently .

Product Science Overview

Introduction

Glutamic-Pyruvate Transaminase (GPT), also known as Alanine Aminotransferase (ALT), is an enzyme that plays a crucial role in amino acid metabolism. It catalyzes the reversible transamination between L-alanine and alpha-ketoglutarate to produce L-glutamate and pyruvate . This enzyme is significant in the intermediary metabolism of glucose and amino acids, making it essential for various physiological processes .

Structure and Function

GPT belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family and has two distinct molecular and genetic forms: one cytoplasmic (GPT1) and one mitochondrial (GPT2) . The enzyme’s activity is dependent on the presence of pyridoxal phosphate, a derivative of vitamin B6, which acts as a coenzyme in the transamination process .

Recombinant Production

Human recombinant GPT is produced using advanced biotechnological methods. The gene encoding GPT is cloned into an expression vector, which is then introduced into a suitable host cell, such as E. coli or mammalian cells. The host cells are cultured under optimal conditions to express the recombinant protein, which is subsequently purified using various chromatographic techniques .

Applications

Recombinant GPT is widely used in research and clinical diagnostics. It serves as a biomarker for liver function tests, as elevated levels of GPT in the serum indicate liver damage or disease . Additionally, it is used in studies related to amino acid metabolism, drug development, and the investigation of metabolic disorders .

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