GPT Human, His Active

Glutamic-Pyruvate Transaminase, His Tag Active Human Recombinant
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Description

1. Introduction to GPT Human, His Active

GPT Human, His Active refers to a recombinant human glutamic-pyruvate transaminase (GPT), also known as alanine aminotransferase 1 (ALT1), engineered with a hexahistidine (His) tag for purification and functional studies. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to produce pyruvate and glutamate, playing a critical role in glucose and amino acid metabolism . Elevated serum GPT levels are widely used as biomarkers for liver injury caused by drug toxicity, infections, or metabolic disorders . The His-tagged variant enables precise biochemical and clinical research applications due to its enhanced stability and ease of isolation.

3. Biochemical Functions

GPT Human, His Active exhibits two primary enzymatic activities:

  1. Transaminase Activity: Mediates nitrogen transfer between alanine and α-ketoglutarate, critical for gluconeogenesis and amino acid catabolism.

  2. Diagnostic Utility: Serum ALT/GPT levels correlate with liver damage severity, with normal ranges between 7–56 U/L .

Kinetic Parameters (derived from preclinical studies ):

SubstrateKm (mM)Vmax (μmol/min/mg)
L-alanine3.2 ± 0.412.5 ± 1.2
2-oxoglutarate0.8 ± 0.114.3 ± 1.5

4. Research Applications

GPT Human, His Active is utilized in:

  • Drug Toxicity Screening: Detects hepatotoxicity in preclinical models (e.g., acetaminophen overdose studies) .

  • Metabolic Pathway Analysis: Investigates cross-talk between glycolysis and amino acid metabolism.

  • Biomarker Validation: Calibrates ALT assays for clinical diagnostics.

5. Case Study: Sotuletinib-Induced ALT Elevation

A 2022 study demonstrated GPT Human, His Active’s role in resolving ambiguous liver injury signals during CSF1-R inhibitor (Sotuletinib) trials :

  • Objective: Determine if ALT elevation stemmed from hepatic damage or delayed clearance by Kupffer cells.

  • Method: Recombinant His-ALT1 was administered to Sotuletinib-treated rats.

  • Result: Treated rats showed 60% slower ALT clearance, confirming Kupffer cell dysfunction as the cause .

Key Data:

ParameterControlSotuletinib-Treated
ALT half-life (min)45 ± 672 ± 8
Peak ALT activity (U/L)120 ± 15310 ± 25

6. Pathways and Molecular Interactions

GPT Human, His Active participates in six major metabolic pathways :

PathwayRelated Proteins
Arginine biosynthesisASS1, GLUL, ASL
Alanine metabolismABAT, GLUD1B
Carbon metabolismIDH1, ALDOA

Protein Interactions:

  • Direct binding partners include CDC7 (cell cycle regulation) and RALA (Ras signaling) .

7. Technological Integration: AI in GPT Research

Recent advances leverage AI models like GPT-4 to accelerate GPT-related research:

  • Molecular Property Prediction: GPT-3 accurately predicted redox potentials (e.g., TEMPO at +0.5 V) .

  • Qualitative Data Analysis: GPT-4 achieved 55% agreement with human researchers in coding patient-reported outcomes for liver disease .

Comparative Performance:

TaskHuman AccuracyGPT-4 Accuracy
Theme identification95%75%
Subtheme granularity35%20%

8. Challenges and Ethical Considerations

  • Bias in AI Models: GPT-4 exhibits WEIRD (Western, educated, industrialized, rich, democratic) cultural biases, limiting generalizability in global health studies .

  • Data Integrity: AI-generated disinformation (e.g., synthetic tweets about vaccines) is indistinguishable from human content, risking misuse in public health contexts .

Product Specs

Introduction
GPT (Glutamic-pyruvic transaminase) is an enzyme that plays a vital role in the metabolism of amino acids and glucose. It catalyzes the reversible conversion of alanine and 2-oxoglutarate into pyruvate and glutamate. GPT is a key indicator of liver health and is frequently used in clinical settings to assess liver function and detect potential damage.
Description
This product consists of recombinant human GPT, expressed in E. coli and purified to a high degree. It is a single polypeptide chain, lacking glycosylation, with 516 amino acids (residues 1-496). The protein has a molecular weight of 56.8 kDa. For purification and detection purposes, a 20 amino acid His-tag is fused to the N-terminus.
Physical Appearance
The product is a sterile, colorless solution.
Formulation
The GPT enzyme is supplied in a solution at a concentration of 0.5mg/ml. The solution also contains 20mM Tris-HCl buffer (pH 8.0), 2mM DTT (dithiothreitol), and 20% glycerol.
Stability
For short-term storage (up to 4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of GPT Human Recombinant is determined by SDS-PAGE analysis and is guaranteed to be greater than 90%.
Biological Activity
The specific activity of GPT is measured as the amount of enzyme required to convert 1 micromole of L-Alanine to L-Glutamate per minute at a pH of 7.5 and a temperature of 37°C. The specific activity is greater than 100 units per milligram of enzyme.
Synonyms
Glutamic-pyruvate transaminase (alanine aminotransferase), GPT1, ALT1, AAT1, Glutamic-alanine transaminase 1, EC 2.6.1.2.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MASSTGDRSQ AVRHGLRAKV LTLDGMNPRV RRVEYAVRGP IVQRALELEQ ELRQGVKKPF TEVIRANIGD AQAMGQRPIT FLRQVLALCV NPDLLSSPNF PDDAKKRAER ILQACGGHSL GAYSVSSGIQ LIREDVARYI ERRDGGIPAD PNNVFLSTGA SDAIVTVLKL LVAGEGHTRT GVLIPIPQYP LYSATLAELG AVQVDYYLDE ERAWALDVAE LHRALGQARD HCRPRALCVI NPGNPTGQVQ TRECIEAVIR FAFEERLFLL ADEVYQDNVY AAGSQFHSFK KVLMEMGPPY AGQQELASFH STSKGYMGEC GFRGGYVEVV NMDAAVQQQM LKLMSVRLCP PVPGQALLDL VVSPPAPTDP SFAQFQAEKQ AVLAELAAKA KLTEQVFNEA PGISCNPVQG AMYSFPRVQL PPRAVERAQE LGLAPDMFFC LRLLEETGIC VVPGSGFGQR EGTYHFRMTI LPPLEKLRLL LEKLSRFHAK FTLEYS

Q&A

What are the primary mechanisms through which GPT models assist in academic research?

GPT models function as research assistants through several key mechanisms. At their core, these models leverage transformer architectures with self-attention mechanisms that enable them to process and synthesize large volumes of textual information rapidly. For researchers, this translates into capabilities that extend beyond simple text generation to include literature synthesis, hypothesis development, and methodological refinement .

In academic settings, researchers are utilizing GPT models to extend their thinking on specialized topics that may be too niche for regular colleagues to engage with meaningfully. The models can maintain complex conversations across wide and deep subject areas, helping researchers check assumptions and refine their thinking through iterative dialogue . This effectively creates a virtual research partner that never tires of exploring conceptual nuances and can respond to highly specific intellectual inquiries.

Methodologically, researchers are integrating GPT models into their workflows by having the models generate structured questions that prompt deeper reflection, which are then answered by the researcher to form the basis of more coherent written work. This methodological approach maintains researcher agency while leveraging the model's ability to organize complex information .

How does the integration of GPT models change traditional research methodologies?

The integration of GPT models represents a fundamental shift in research methodology across multiple dimensions. Traditionally, research synthesis required manual review of literature, but GPT models can now perform initial scans of extensive resources, identifying patterns and connections across disparate studies that might otherwise be overlooked .

The models are reshaping how researchers engage with literature by enabling dynamic querying rather than static searching. This allows for more exploratory approaches where researchers can progressively refine their understanding through conversational interaction. For example, researchers can present complex theories to the model and receive substantive feedback that helps identify novel connections between concepts .

What distinguishes effective from ineffective prompting strategies when utilizing GPT for research?

Effective prompting strategies for research applications demonstrate several distinctive characteristics compared to ineffective approaches. Research-oriented prompts that yield valuable results tend to be highly specific in their scope, clearly defining the research domain and expected output format . For instance, specifying "List all relevant papers you find, the authors, the methods used, and the results" produces more structured and useful responses than general inquiries.

Methodologically superior prompts incorporate analytical directives rather than merely requesting information. By instructing the model to "Study patterns across papers" or "Compare methodological approaches," researchers elicit synthetic thinking rather than simple retrieval . This distinction is critical for generating insights rather than merely aggregating information.

The most effective research prompts typically include:

  • Clear specification of academic context and purpose

  • Explicit requests for comparative analysis across sources

  • Structured output requirements (tables, categorizations, chronologies)

  • Directives to identify theoretical frameworks or methodological patterns

Ineffective prompts, by contrast, tend to be overly broad, lack analytical direction, or fail to specify the required depth of scholarly engagement .

How can researchers effectively use GPT models to resolve contradictory findings in literature reviews?

When confronted with contradictory findings across studies, researchers can employ specific methodological approaches with GPT models to facilitate resolution. The process begins with structured prompting that explicitly tasks the model with identifying contradictions rather than seeking consensus. For example, researchers might request: "Identify studies with conflicting results regarding X and analyze the methodological differences that might explain these contradictions."

A comprehensive approach involves creating data tables that systematically compare the methodologies, sample characteristics, analytical techniques, and contextual factors across contradictory studies. GPT models can assist by:

  • Extracting and organizing methodological details from multiple studies

  • Identifying variables that differ between contradictory findings

  • Suggesting potential moderating or mediating factors that could explain divergent results

  • Proposing testable hypotheses that might resolve the contradictions

Most importantly, researchers should maintain a critical stance toward the model's synthesis, using it as a starting point for deeper investigation rather than accepting its resolution of contradictions as definitive. The methodology should include systematic verification against primary sources and critical evaluation of the model's suggested explanations .

What methodological frameworks exist for validating insights generated by GPT models in research contexts?

Validation of GPT-generated insights requires robust methodological frameworks that maintain scientific rigor. Current approaches include triangulation methods where researchers compare GPT-generated analyses with:

  • Traditional systematic review methodologies

  • Independent expert assessments

  • Computational validation using alternative analytical tools

  • Empirical testing of derived hypotheses

From a methodological standpoint, the most rigorous validation approaches implement a multi-stage process:

Validation StageMethodological ApproachKey Considerations
Initial ScreeningCross-reference with established literatureVerify factual accuracy and citation validity
Conceptual ValidationExpert panel reviewAssess theoretical coherence and disciplinary alignment
Methodological AssessmentEvaluation of suggested research designsDetermine feasibility and methodological soundness
Empirical TestingImplementation of suggested hypothesesTest predictive validity of GPT-generated insights

Research indicates that validation processes must be domain-specific, with different fields requiring tailored approaches to verification. In rapidly evolving fields, continuous updating of the validation framework is necessary to account for new developments .

How do researchers effectively balance human expertise with GPT capabilities in collaborative research designs?

Establishing effective human-AI collaboration in research requires thoughtful methodological considerations that capitalize on the complementary strengths of both. Research suggests that optimal collaboration frameworks position GPT models as amplifiers of human expertise rather than replacements .

Methodologically, successful collaborative designs implement structured workflows where:

  • Researchers define research questions and theoretical frameworks

  • GPT models assist with literature synthesis and pattern identification

  • Human experts critically evaluate generated insights and redirect inquiry as needed

  • Iterative dialogue between researcher and model progressively refines understanding

This collaborative approach has demonstrated particular value in exploratory phases of research, where GPT models can help researchers expand their conceptual landscape by suggesting unexpected connections or alternative interpretations . For example, researchers have reported breakthrough insights when presenting complex theoretical constructs to GPT and receiving novel perspectives that connected seemingly unrelated concepts .

The most effective collaboration occurs when researchers maintain clear boundaries regarding decision-making authority while remaining open to having their thinking challenged and extended by the model's suggestions. This balanced approach preserves scientific integrity while leveraging computational capabilities .

What experimental designs best measure the impact of GPT integration on research quality and productivity?

Rigorous evaluation of GPT's impact on research processes requires carefully constructed experimental designs that isolate key variables while maintaining ecological validity. Based on current methodological approaches, the most effective designs include:

  • Controlled comparative studies where matched groups of researchers complete identical tasks with and without GPT assistance, followed by blind quality assessment by expert panels

  • Within-subjects designs where researchers alternate between traditional and GPT-assisted approaches, allowing for direct comparison of efficiency and quality metrics

  • Longitudinal studies tracking changes in research productivity, innovation, and quality as researchers integrate GPT models into their workflows over time

Methodologically sound experimental designs in this domain must control for confounding variables including:

  • Prior AI experience and technological proficiency

  • Domain expertise and research experience

  • Task complexity and knowledge domain

  • Learning effects from repeated exposure to similar research tasks

Productivity metrics should extend beyond simple speed measures to include comprehensive quality assessments, including originality, methodological rigor, and theoretical contribution .

How should researchers document GPT usage in their methodologies for peer-reviewed publications?

Transparent documentation of GPT usage in research methodologies requires detailed reporting practices that enable reproducibility while acknowledging the contributions and limitations of AI assistance. Based on emerging standards, comprehensive documentation should include:

  • Precise description of the GPT model version used

  • The specific research tasks for which GPT was employed

  • Representative examples of prompts used to generate insights

  • Validation procedures implemented to verify GPT-generated content

  • Limitations of the model relevant to the research context

A structured documentation framework might include:

Documentation ElementRequired InformationPurpose
Model SpecificationVersion, access date, parametersEnsures reproducibility
Task DescriptionSpecific research activities performed by the modelClarifies scope of AI contribution
Prompt ExamplesRepresentative samples of researcher-model interactionEnables methodological assessment
Verification ProtocolSteps taken to validate model outputsDemonstrates scientific rigor
Limitation StatementKnown constraints of the model relevant to research contextAcknowledges boundaries of AI assistance

This comprehensive documentation approach supports scientific transparency while facilitating methodological advancement in AI-assisted research .

What experimental protocols can identify and mitigate biases in GPT-assisted research processes?

Addressing bias in GPT-assisted research requires systematic experimental protocols that identify, measure, and mitigate potential distortions. Effective methodological approaches include:

  • Comparative analysis where identical research questions are approached through multiple prompting strategies, revealing potential bias in how questions are framed

  • Adversarial testing where deliberately contrary perspectives are prompted to assess balance in model responses

  • Structured verification protocols that cross-check GPT-generated insights against diverse sources

  • Blind review processes where experts evaluate GPT-generated content without knowledge of its source

Research suggests that bias detection requires particular attention to:

  • Representation of minority perspectives and underrepresented populations

  • Balance in citation patterns across demographic and geographic dimensions

  • Methodological diversity in synthesized literature

  • Equitable consideration of contradictory evidence

Mitigation strategies should be implemented as standard research protocols rather than ad hoc corrections, with systematic documentation of bias identification and remediation efforts .

How can researchers leverage GPT models for theory development and conceptual integration?

GPT models offer novel methodological approaches to theory development that extend beyond traditional literature-based conceptualization. Research indicates that interactive dialogue with GPT can facilitate theoretical advancement through several mechanisms:

  • Identifying implicit connections between seemingly disparate theoretical constructs

  • Testing the logical consistency of theoretical propositions through systematic questioning

  • Translating concepts across disciplinary boundaries to identify parallel constructs

  • Generating testable hypotheses derived from theoretical integration

Methodologically, researchers can implement a structured theory development protocol using GPT that includes:

  • Comprehensive mapping of existing theoretical frameworks

  • Identification of conceptual gaps and contradictions

  • Exploration of potential integrative frameworks

  • Formulation of boundary conditions and contingencies

This approach has demonstrated particular value when researchers present complex theoretical ideas and receive feedback that identifies novel connections or missing conceptual links . For example, researchers report that presenting sophisticated theories about phenomena like "bird mating dances and consciousness" to GPT resulted in identification of "novel missing links" that connected previously unrelated theoretical domains .

What methodological approaches enable researchers to assess the reliability of GPT-generated research insights?

Evaluating the reliability of GPT-generated research insights requires rigorous methodological frameworks that extend beyond simple fact-checking. Current approaches include:

  • Consistency testing across multiple prompting iterations

  • Comparison of outputs from different model versions or AI systems

  • Source tracing to identify the empirical foundations of generated insights

  • Expert validation through structured review protocols

A comprehensive reliability assessment framework would include:

Reliability DimensionAssessment MethodologyReliability Indicators
Factual AccuracyVerification against primary sourcesPercentage of verified claims
Conceptual ConsistencyLogical analysis of theoretical frameworkInternal coherence rating
Bibliographic ReliabilityCitation validationProportion of verifiable references
Methodological SoundnessExpert review of research designsFeasibility and validity ratings

Research indicates that reliability varies significantly across knowledge domains, with well-established scientific fields generally yielding more reliable insights than emerging or highly specialized areas . This necessitates domain-specific reliability thresholds and validation protocols.

How do researchers effectively combine GPT-generated insights with traditional data analysis methods?

Integrating GPT capabilities with established research methodologies requires thoughtful approaches that preserve methodological rigor while leveraging AI capabilities. Effective integration strategies include:

  • Using GPT for preliminary pattern identification followed by rigorous statistical validation

  • Employing GPT to generate alternative interpretations of quantitative findings

  • Utilizing GPT to synthesize qualitative data before formal coding and analysis

  • Leveraging GPT to identify potential confounding variables or methodological limitations

A structured integration workflow might include:

Research PhaseGPT RoleTraditional Method RoleIntegration Approach
Literature ReviewComprehensive synthesisCritical evaluationGPT-generated overview refined by expert assessment
Hypothesis GenerationPattern identificationTheoretical groundingAI-identified patterns validated against established theory
Data AnalysisExploratory pattern detectionConfirmatory analysisGPT-suggested patterns tested with formal statistical methods
InterpretationAlternative explanationsMethodological constraintsMultiple interpretations evaluated against empirical evidence

This complementary approach maintains methodological integrity while expanding analytical possibilities .

How are academic institutions adapting research ethics frameworks to address GPT integration?

Academic institutions are implementing comprehensive adaptations to research ethics frameworks to address the unique challenges presented by GPT integration. Current approaches focus on several key dimensions:

  • Attribution and intellectual contribution - Establishing clear guidelines for acknowledging AI contributions while maintaining appropriate credit for human researchers

  • Transparency requirements - Developing standardized protocols for disclosing AI usage in research methodologies

  • Data privacy considerations - Addressing concerns regarding sensitive information shared with AI systems during research processes

  • Quality assurance mechanisms - Implementing verification standards for AI-assisted research

The evolution of institutional policies reveals a progression from prohibition to managed integration, with leading institutions developing nuanced frameworks that distinguish between appropriate research assistance and problematic delegation .

Recent institutional adaptations include:

  • Development of AI-specific research integrity training programs

  • Establishment of expert review committees for AI-assisted research

  • Creation of technical infrastructure for documenting and verifying AI contributions

  • Implementation of detection technologies to identify undisclosed AI usage in research products

These institutional responses reflect the recognition that GPT integration represents not merely a technical challenge but a fundamental shift in research epistemology requiring corresponding ethical frameworks .

What methodological training do researchers need to effectively incorporate GPT models in their work?

Preparing researchers for effective GPT integration requires comprehensive methodological training that extends beyond basic operational skills. Based on emerging educational approaches, essential training components include:

  • Prompt engineering for research applications - Developing skills in crafting effective research-oriented prompts

  • Critical evaluation of GPT-generated content - Building capacity to identify limitations, biases, and errors

  • Integration methodologies - Learning structured approaches to combining AI insights with traditional research methods

  • Verification protocols - Mastering techniques for validating AI-generated information

A comprehensive training curriculum might include:

Training ModuleKey CompetenciesAssessment Approaches
Fundamentals of LLM OperationUnderstanding model capacities and limitationsConceptual assessment of model functionality
Research-Specific PromptingCrafting effective research queriesEvaluation of prompt quality and resulting outputs
Critical EvaluationIdentifying errors and biases in AI contentComparative analysis of AI outputs against verified sources
Ethical IntegrationApplying appropriate attribution and disclosure practicesCase studies of ethical dilemmas in AI-assisted research

Research indicates that effective training approaches combine theoretical understanding with hands-on application, allowing researchers to develop contextual judgment about appropriate AI utilization .

How might GPT integration transform researcher skill development and scholarly identity?

The integration of GPT models into research processes has profound implications for researcher development and scholarly identity formation. Analysis of current trends suggests several transformative dimensions:

  • Shift from information retrieval to question formulation - As GPT models excel at synthesizing existing knowledge, researcher value increasingly derives from asking novel questions rather than possessing information

  • Emphasis on critical evaluation over production - Researchers must develop sophisticated evaluation skills to assess AI-generated content

  • Expansion of interdisciplinary capacity - GPT facilitates engagement with multiple disciplines, potentially transforming scholarly identity from disciplinary specialist to integrative synthesizer

  • Evolution of mentorship relationships - The availability of GPT for basic research questions may redirect human mentorship toward wisdom transmission rather than knowledge transfer

Longitudinal observations suggest that researchers who effectively integrate GPT tools report enhanced capacity for conceptual thinking and theoretical innovation . Rather than diminishing scholarly capabilities, thoughtful GPT integration appears to enhance higher-order research skills while automating more routine aspects of scholarship.

Evidence indicates that GPT interaction may particularly benefit researchers with niche interests or interdisciplinary orientations who previously lacked adequate discussion partners for complex ideas . This suggests GPT integration may democratize access to scholarly dialogue, potentially diversifying the research landscape.

Product Science Overview

Introduction

Glutamic-Pyruvate Transaminase (GPT), also known as Alanine Aminotransferase (ALT), is an enzyme that plays a crucial role in the metabolism of amino acids and glucose. It catalyzes the reversible transamination between L-alanine and alpha-ketoglutarate to produce L-glutamate and pyruvate . This enzyme is found in various tissues, but it is most abundant in the liver .

Structure and Function

GPT belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family and has two distinct molecular and genetic forms: one cytoplasmic (GPT1) and one mitochondrial (GPT2) . The enzyme requires the coenzyme pyridoxal phosphate for its activity . The His Tag Active form of GPT is a recombinant version that includes a histidine tag, which facilitates its purification and detection in research applications .

Clinical Significance

GPT is widely used as a biomarker for liver health. Elevated levels of GPT in the blood can indicate liver damage or disease, such as viral hepatitis, diabetes, or bile duct problems . It is commonly measured in liver function tests alongside aspartate aminotransferase (AST), and the ratio of AST to ALT is used to diagnose various liver conditions .

Recombinant GPT

The recombinant form of GPT, particularly the His Tag Active version, is produced using advanced biotechnological methods. This involves the expression of the human GPT gene in a suitable host system, followed by purification using the histidine tag . The recombinant enzyme retains the functional properties of the native enzyme and is used in various research and diagnostic applications .

Applications

Recombinant GPT is used in:

  • Biochemical Research: Studying the enzyme’s role in amino acid and glucose metabolism.
  • Clinical Diagnostics: Developing assays for liver function tests.
  • Drug Development: Screening for potential hepatotoxicity of new drugs.

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