HBsAg adw CHO
Description
Immunological and Functional Properties
HBsAg adw CHO retains structural and antigenic features critical for immune recognition:
Epitope Composition
The adr subtype contains distinct epitopes recognized by host immune systems:
CHO-derived HBsAg adw2 particles include pre-S1 and pre-S2 sequences, enhancing immune recognition compared to yeast-derived vaccines .
Diagnostic Uses
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Serological Testing: Detects HBV infection via ELISA or immunochromatography .
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Vaccine Development: Serves as a reference antigen for evaluating vaccine efficacy .
Research Insights
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Immunogenicity in Vaccines:
CHO-derived HBsAg adw2 elicits stronger antibody responses than yeast-derived vaccines due to: -
CTL Response Variability:
Comparative Analysis of HBsAg Subtypes
| Feature | CHO-Derived adw2 | Yeast-Derived adw2 | adr (CHO) |
|---|---|---|---|
| Pre-S Proteins | Includes pre-S1 and pre-S2 | Absent | Includes pre-S1 and pre-S2 |
| Epitope 1 (208–215) | ILSPFIPL | IVSPFIPL | ILSPFLPL |
| Epitope 2 (190–197) | VWLSVIWM | VWLSAIWM | VWLSVIWM |
| Immunogenicity | Strong antibody response | Moderate response | Strong antibody response |
Stability and Handling
Research Implications
Product Specs
Hepatitis B surface antigen (HBsAg) is a protein found on the surface of the hepatitis B virus (HBV). It is used as a marker to detect HBV infection. HBsAg is also known as the Australia antigen. The presence of HBsAg in the blood indicates current or recent infection with HBV. HBsAg is an important marker for diagnosing and monitoring hepatitis B infection.
Recombinant Hepatitis B Surface Antigen, Adw subtype, is a 23 kDa protein produced in CHO cells. It is composed of 226 amino acids and purified using a proprietary chromatographic technique. This recombinant protein represents the HBsAg protein, specifically the Adw subtype, and is suitable for research and diagnostic applications related to hepatitis B.
Sterile Filtered clear solution.
The HBsAg adw CHO solution is formulated in PBS with a pH of 6.5 and contains 0.01% sodium azide (NaN3) as a preservative.
For short-term storage (up to 4 weeks), the solution can be stored at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. Adding a carrier protein like 0.1% HSA or BSA can further enhance stability during long-term storage. To maintain the product's integrity, avoid repeated freezing and thawing cycles.
The purity of the protein is greater than 95% as determined by SDS-PAGE analysis using a 12% gel and Coomassie blue staining.
Chinese Hamster Ovary Cells (CHO).
Product Science Overview
Hepatitis B is a viral infection that affects the liver and can lead to both acute and chronic diseases. The Hepatitis B virus (HBV) is a member of the Hepadnaviridae family and has a partially double-stranded circular DNA genome. One of the key components of the HBV is the Hepatitis B surface antigen (HBsAg), which is used in the development of vaccines and diagnostic tests.
HBsAg is a protein that forms part of the outer surface of the HBV. It plays a crucial role in the virus’s ability to infect liver cells and is a primary target for the immune response. The antigenic heterogeneity of HBsAg is characterized by different subtypes, including adw, ayw, adr, and ayr . The adw subtype is one of the most commonly studied and utilized in research and vaccine development.
Recombinant HBsAg is produced using genetic engineering techniques. The gene encoding the HBsAg is inserted into a host cell, such as Chinese Hamster Ovary (CHO) cells, which then express the antigen. This method allows for the production of large quantities of HBsAg with high purity and consistency .
CHO cells are widely used in biotechnology for the production of recombinant proteins. They are preferred due to their ability to grow in suspension cultures, high protein expression levels, and proper protein folding and post-translational modifications. The recombinant HBsAg produced in CHO cells is typically purified using techniques such as ionic exchange chromatography and size exclusion chromatography .
Recombinant HBsAg is used in various applications, including:
- Vaccine Development: It is a key component in the formulation of Hepatitis B vaccines, which are used to prevent HBV infection.
- Diagnostic Tests: HBsAg is used in serological tests to diagnose Hepatitis B infection. The presence of HBsAg in the blood indicates an active HBV infection.
- Research: Recombinant HBsAg is used in research to study the immune response to HBV and to develop new therapeutic strategies .
