HBsAg ayw, 31kDa

Hepatitis B Surface Antigen ayw Recombinant

Recombinant HBsAg ayw full length, a 31kDa protein, is derived from the HBV 320 genome and is cloned with a 6xHis Tag.
Shipped with Ice Packs
Cat. No.
BT8049
Source
Escherichia Coli.
Appearance
The solution is clear and has been sterilized through filtration.

HBsAg preS1

Hepatitis B Surface Antigen, preS1 Recombinant

Recombinant Hepatitis B Surface Antigen preS1, derived from E. coli, is a non-glycosylated polypeptide chain consisting of 119 amino acids. It has a molecular weight of 12.6 kDa.
Shipped with Ice Packs
Cat. No.
BT8158
Source
Escherichia Coli.

HBsAg preS2

Hepatitis B Surface Antigen, preS2 Recombinant

Recombinant Hepatitis B Surface Antigen preS2 is a single, non-glycosylated polypeptide chain consisting of 55 amino acids, with an approximate molecular weight of 5.7 kDa. It has been purified using a proprietary chromatographic technique.
Shipped with Ice Packs
Cat. No.
BT8210
Source
E.coli.

HBsAg adr CHO

Hepatitis B Surface Antigen, adr CHO Recombinant

Recombinant HBsAg adr, with a molecular weight of approximately 23-27kDa, is produced in Chinese Hamster Ovary (CHO) cells.
Shipped with Ice Packs
Cat. No.
BT7681
Source
CHO.
Appearance
A clear solution that has been sterilized by filtration.

HBsAg adw

Hepatitis B Surface Antigen, adw Recombinant

HBsAg adw, produced in Pichia Pastoris, exhibits an approximate molecular weight of 24.0 kDa, as determined by SDS-PAGE analysis.
Shipped with Ice Packs
Cat. No.
BT7763
Source
Pichia Pastoris.
Appearance
A sterile-filtered solution with a pale appearance.

HBsAg adw CHO

Hepatitis B Surface Antigen Adw subtype Recombinant, CHO

Recombinant Hepatitis B Surface Antigen, Adw subtype, is a 23 kDa protein produced in CHO cells. It is composed of 226 amino acids and purified using a proprietary chromatographic technique. This recombinant protein represents the HBsAg protein, specifically the Adw subtype, and is suitable for research and diagnostic applications related to hepatitis B.

Shipped with Ice Packs
Cat. No.
BT7835
Source

Chinese Hamster Ovary Cells (CHO).

Appearance

Sterile Filtered clear solution.

HBsAg adw2

Hepatitis B Surface Antigen, adw2 Recombinant

Produced in E. coli, HBsAg subtype adw2 is a non-glycosylated polypeptide chain consisting of 226 amino acids. With a molecular weight of approximately 23.0kDa, it presents as a monomer band on SDS-PAGE, with minor dimer (46kDa) and trimer (69kDa) formations. The HBsAg is tagged with a 6 His tag at the C-terminus and purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT7912
Source
Escherichia Coli.
Appearance
Colorless, sterile, and filtered solution.

HBsAg ayw

Hepatitis B Surface Antigen, ayw Recombinant

Recombinant full-length HBsAg ayw protein was expressed in Saccharomyces cerevisiae and purified. This protein is a multimer on SDS-PAGE, with monomers, dimers, trimers, etc. present. The protein has a molecular weight of 18kDa (ayw) and is fused to a 6 His Tag at the C-terminus.
Shipped with Ice Packs
Cat. No.
BT7966
Source
Saccharomyces cerevisae.
Appearance
Clear, colorless, and sterile-filtered solution.
Definition and Classification

Hepatitis B surface antigen (HBsAg), also known as the Australia antigen, is a protein found on the surface of the hepatitis B virus (HBV). It is a key marker used in the diagnosis of hepatitis B infection . HBsAg is classified into three isoforms based on their size: large (LHB), middle (MHB), and small (SHB) surface proteins .

Biological Properties

Key Biological Properties: HBsAg is a multifunctional glycoprotein that plays a crucial role in the viral life cycle and immune response. It is composed of 226 amino acids and has both hydrophilic and hydrophobic domains .

Expression Patterns: HBsAg is expressed on the surface of HBV and is released into the bloodstream during infection. It is present in high concentrations in the blood of infected individuals .

Tissue Distribution: HBsAg is primarily found in the liver, where HBV replicates. It can also be detected in other tissues and body fluids, including blood, saliva, and semen .

Biological Functions

Primary Biological Functions: HBsAg is responsible for the initial attachment of HBV to hepatocytes, facilitating viral entry into the host cells . It also plays a role in the assembly and release of viral particles.

Role in Immune Responses: HBsAg elicits both humoral and cellular immune responses. It is a major target for neutralizing antibodies and cytotoxic T cells, which help control HBV infection .

Pathogen Recognition: HBsAg is recognized by the immune system as a foreign antigen, triggering an immune response aimed at eliminating the virus .

Modes of Action

Mechanisms with Other Molecules and Cells: HBsAg interacts with various cellular receptors to facilitate viral entry and replication. It binds to the sodium taurocholate co-transporting polypeptide (NTCP) receptor on hepatocytes .

Binding Partners: HBsAg binds to host cell receptors and other viral proteins, such as the hepatitis B core antigen (HBcAg), to form the viral envelope .

Downstream Signaling Cascades: Upon binding to host cell receptors, HBsAg triggers signaling pathways that promote viral replication and immune evasion .

Regulatory Mechanisms

Transcriptional Regulation: The expression of HBsAg is regulated by viral promoters and enhancers. The HBV genome contains regulatory elements that control the transcription of HBsAg mRNA .

Post-Translational Modifications: HBsAg undergoes various post-translational modifications, including glycosylation, which are essential for its proper folding, stability, and function .

Applications

Biomedical Research: HBsAg is widely used in research to study HBV biology, pathogenesis, and immune responses. It serves as a model antigen for developing vaccines and antiviral therapies .

Diagnostic Tools: HBsAg detection is a cornerstone of HBV diagnosis. It is used in serological assays to screen for HBV infection and monitor treatment efficacy .

Therapeutic Strategies: HBsAg is a target for therapeutic vaccines and antiviral drugs aimed at achieving functional cure of chronic hepatitis B. Strategies include inducing HBsAg seroclearance and inhibiting its production .

Role in the Life Cycle

Development to Aging and Disease: HBsAg plays a critical role throughout the HBV life cycle. It is involved in the initial infection, chronic infection, and disease progression. The persistence of HBsAg in the blood is associated with chronic hepatitis B, liver cirrhosis, and hepatocellular carcinoma .

© Copyright 2024 Thebiotek. All Rights Reserved.