Coronavirus 2019 3CL Protease Recombinant
Recombinant Coronavirus 2019 3CL Protease, with an approximate molecular weight of 33.8 kDa, was produced using E. coli and subsequently purified. This protein comprises 306 amino acids and was purified using a proprietary chromatographic method.
Escherichia Coli.
White lyophilized (freeze-dried) powder that has been filtered.
Coronavirus 2019 Nucleocapsid (201-419 a.a.) Recombinant
This recombinant protein, expressed in E. coli, encompasses the C-terminal domain of the Coronavirus 2019 nucleocapsid protein (amino acids 201-419). It is fused with a 6xHis tag at the C-terminus and has a molecular weight of 23.9 kDa.
Coronavirus 2019 Nucleocapsid (329 a.a.), Recombinant
This recombinant protein is derived from E. coli and encompasses the C-terminal region (329 amino acids) of the Coronavirus 2019 Nucleocapsid protein. It is fused with a GST-6xHis tag at the N-terminal and exhibits a molecular weight of 63.5 kDa.
E.Coli.
The product appears as a clear solution that has undergone sterile filtration.
Coronavirus 2019 Nucleocapsid Mosaic Recombinant
This E. coli-derived recombinant protein consists of the full-length Coronavirus 2019 nucleocapsid protein with immunodominant regions (full-length N-antigen), fused to a C-terminal 6xHis tag.
Coronavirus 2019 Nucleocapsid Recombinant
This product consists of the full-length nucleocapsid protein of the 2019 Coronavirus (Gene bank: MN908947), produced in E. coli. It is recombinantly designed with a C-terminal 6xHis tag and exhibits a molecular weight of 48 kDa as observed on SDS-PAGE.
Coronavirus 2019 Omicron Full Length Recombinant
This recombinant protein, produced in E. coli, encompasses the full-length nucleocapsid protein of the Omicron variant of SARS-CoV-2. It is fused with a 6xHis tag at its N-terminus and has an approximate molecular weight of 48 kDa.
Coronavirus 2019 Spike (800-1000 a.a.) Recombinant
This product consists of the immunodominant portion of the Coronavirus 2019 Spike protein (amino acids 800-1000), produced in E. coli and purified. A 6xHis tag is attached to the C-terminus to facilitate purification and detection.
Coronavirus 2019 Spike E-Mosaic Recombinant
This recombinant protein is derived from E. coli and contains immunodominant regions of the Coronavirus 2019 spike protein's Envelope Mosaic region. It has a molecular weight of 40.5 kDa and is fused with a 6xHis tag at the C-terminus.
Coronavirus 2019 Spike S1 (200-800 a.a.) Recombinant
This recombinant protein, expressed in E. coli, encompasses the S1 subunit (amino acids 200-800) of the SARS-CoV-2 Spike protein. It features a C-terminal 6xHis tag for purification and detection purposes.
Coronavirus 229E Recombinant
Recombinant Human Coronavirus 229E, produced in E. coli, is a 359 amino acid protein containing the nucleocapsid's immunodominant regions. A 6xHis tag is attached to the protein's C-terminal, and it undergoes purification using standard chromatographic methods.
Severe Acute Respiratory Syndrome (SARS) is a viral respiratory illness caused by a coronavirus known as SARS-CoV. It was first identified in 2003 during an outbreak that began in China and spread to other countries . SARS-CoV belongs to the family Coronaviridae, which is divided into four genera: Alpha, Beta, Gamma, and Delta coronaviruses . SARS-CoV is classified under the Betacoronavirus genus.
Key Biological Properties: SARS-CoV is an enveloped, positive-sense, single-stranded RNA virus. It has a crown-like appearance due to spike proteins on its surface .
Expression Patterns and Tissue Distribution: SARS-CoV primarily infects the respiratory tract, but it can also affect other organs such as the gastrointestinal tract, liver, and kidneys . The virus binds to the angiotensin-converting enzyme 2 (ACE2) receptor, which is widely distributed in various tissues, including the lungs, heart, and intestines .
Primary Biological Functions: The primary function of SARS-CoV is to replicate within host cells. The virus hijacks the host’s cellular machinery to produce viral RNA and proteins, leading to the assembly of new virions .
Role in Immune Responses and Pathogen Recognition: SARS-CoV triggers an immune response by activating various immune cells and signaling pathways. The spike protein of the virus is recognized by the host’s immune system, leading to the production of neutralizing antibodies .
Mechanisms with Other Molecules and Cells: SARS-CoV enters host cells by binding to the ACE2 receptor and undergoing proteolytic cleavage by host cell proteases such as TMPRSS2 . This facilitates viral entry and fusion with the host cell membrane.
Binding Partners and Downstream Signaling Cascades: The binding of SARS-CoV to ACE2 triggers downstream signaling cascades that modulate immune responses and inflammation. The virus can also evade immune detection by interfering with interferon signaling pathways .
Transcriptional Regulation: The expression of SARS-CoV genes is tightly regulated by viral and host factors. Transcription factors such as SP1 and HNF4α play crucial roles in regulating the expression of the ACE2 receptor, which is essential for viral entry .
Post-Translational Modifications: SARS-CoV proteins undergo various post-translational modifications, including phosphorylation, glycosylation, and ubiquitination, which are critical for viral replication and immune evasion .
Biomedical Research: SARS-CoV has been extensively studied to understand viral pathogenesis and host immune responses. This research has led to the development of diagnostic tools and therapeutic strategies .
Diagnostic Tools: Techniques such as polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) are used to detect SARS-CoV infection .
Therapeutic Strategies: Antiviral drugs, monoclonal antibodies, and vaccines have been developed to combat SARS-CoV infection. These therapeutic strategies target various stages of the viral life cycle .
Throughout the Life Cycle: SARS-CoV plays a critical role in the viral life cycle, from initial infection to replication and assembly of new virions. The virus hijacks the host’s cellular machinery to produce viral RNA and proteins, leading to the assembly of new virions . The N protein of SARS-CoV is essential for packaging the viral RNA into new virions and facilitating their release from host cells .