Product List

EBNA1BP2 Human

EBNA1 Binding Protein 2 Human Recombinant

Recombinant Human EBNA1BP2 produced in E. coli is a single polypeptide chain containing 329 amino acids (1-306) and having a molecular mass of 37.2kDa.
EBNA1BP2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT6061
Source
Escherichia Coli.
Appearance
Sterile filtered colorless solution.

EBV EA

Epstein-Barr virus (HHV-4) Early Antigen Recombinant

The E.Coli derived 35.5 kDa recombinant protein contains the HHV-4 Early Antigen Type D, C-terminus regions amino acids 306-390. The protein is fused to a 26kDa GST tag.
Shipped with Ice Packs
Cat. No.
BT6126
Source
Appearance

EBV EBNA1

Epstein-Barr Virus (HHV-4) EBNA1 Recombinant

EBV EBNA1 Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 242 amino acids (His407-Glu641) and having a molecular mass of 25.9kDa.
EBV EBNA1 is fused to a 7 a.a his tag at N-Terminus and is purified by proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT6207
Source

Escherichia Coli.

Appearance

Filtered White lyophilized (freeze-dried) powder.

EBV EBNA1 Mosaic

Epstein-Barr Virus (HHV-4) EBNA1 Mosaic Recombinant

The E.Coli derived recombinant mosaic protein contains the HHV-4 EBNA regions, 1-90, 408-498 amino acids and fused to a 6 aa His Tag at C-terminus and having a molecular weight of 44.2kDa.

Shipped with Ice Packs
Cat. No.
BT6273
Source
Appearance

EBV p18

Epstein-Barr Virus (HHV-4) p18 Recombinant

The E.Coli derived recombinant mosaic protein contains the HHV-4 p18 regions, 1-119 amino acids and fused to a GST-Tag at C-terminus.
Shipped with Ice Packs
Cat. No.
BT6367
Source
Appearance

EBV p18 M

Epstein -Barr virus (HHV-4) p18 Mosaic Recombinant

The E.Coli derived recombinant artificial mosaic protein contains two epitopes from C-end associated in one molecule from the HHV-4 p18 regions.
Shipped with Ice Packs
Cat. No.
BT6454
Source
Escherichia Coli.
Appearance

EBV p18, GST

Epstein - Barr virus (HHV-4) p18 Recombinant, GST Tag

The E.Coli derived recombinant protein contains the HHV-4 p18 regions, having a molecular mass of 35.4kDa which includes GST tag at N-terminus.
Shipped with Ice Packs
Cat. No.
BT6524
Source
Escherichia Coli.
Appearance

EBV p23

Epstein-Barr Virus (HHV-4) p23 Recombinant

The E.Coli derived recombinant protein contains the HHV-4 p23 regions, 1-162 amino acids and fused to a GST-Tag at C-terminus.
Shipped with Ice Packs
Cat. No.
BT6616
Source
Escherichia Coli.
Appearance

Introduction

Definition and Classification

The Epstein-Barr virus (EBV), also known as Human gammaherpesvirus 4, is a member of the Herpesviridae family and is one of the most common viruses in humans . It is a double-stranded DNA virus and was the first identified oncogenic virus, meaning it has the potential to cause cancer . EBV is classified under the genus Lymphocryptovirus within the subfamily Gammaherpesvirinae .

Biological Properties

EBV has a linear double-stranded DNA genome of approximately 184 kilobases, encoding around 100 proteins . The virus primarily infects B lymphocytes and epithelial cells . EBV is highly prevalent, with over 90% of the adult population worldwide having been infected . The virus can establish a latent infection, allowing it to persist in the host for a lifetime . EBV is mainly transmitted through saliva but can also spread through other bodily fluids .

Biological Functions

EBV plays a significant role in immune responses and pathogen recognition. It infects and replicates in epithelial cells and B cells in the oral cavity, leading to the shedding of the virus in the oropharynx and the entry of infected B cells into the bloodstream . The virus can evade the immune system, allowing it to establish a latent infection . EBV is associated with various diseases, including infectious mononucleosis, chronic active EBV infection, EBV-associated autoimmune diseases, and EBV-associated tumors .

Modes of Action

EBV infects B cells and epithelial cells through different glycoproteins. Glycoprotein gp350/220 binds to complement receptor type 2 (CR2/CD21) on B cells, facilitating viral attachment . The virus activates growth transformation programs to drive the proliferation and differentiation of infected B cells into memory B cells . EBV also interacts with antigen-presenting cells, which present antigenic substances to T cells .

Regulatory Mechanisms

The regulation of EBV latency involves various mechanisms, including epigenetic and immune regulation . EBV latency is maintained through the expression of different latent genes at various stages. For example, in latent phase 0, EBV microRNA and EBV-encoded RNA are primarily expressed . In type I latency, EBV-encoded nuclear antigens (EBNA1) and noncoding RNAs are expressed . Type II latency involves the expression of EBNA1, latent membrane proteins (LMP1 and LMP2), and noncoding RNAs . Type III latency occurs in immortalized lymphoblastoid cell lines and involves the expression of all latent proteins .

Applications

EBV has several applications in biomedical research, diagnostic tools, and therapeutic strategies. In research, EBV is used to study viral oncogenesis and immune evasion mechanisms . Diagnostic tools for EBV include serological tests for EBV-specific antibodies and polymerase chain reaction (PCR) for detecting viral DNA . Therapeutic strategies for EBV-associated diseases include antiviral drugs, immunotherapy, and targeted therapies .

Role in the Life Cycle

EBV has a discrete life cycle that includes primary infection, latency, and lytic reactivation . During primary infection, the virus infects epithelial cells and B lymphocytes, leading to the establishment of latency in memory B cells . The virus can remain dormant in the host for a lifetime. Reactivation of the virus can occur under certain conditions, leading to the production of new virions and the potential development of EBV-associated diseases . EBV plays a role in various stages of life, from childhood infections to the development of certain cancers in adulthood .

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