CHIKV E1

Chikungunya E1 Recombinant

Recombinant Chikungunya E1 protein, with a molecular weight of 48kDa, produced in E.coli.

Shipped with Ice Packs
Cat. No.
BT177
Source

Escherichia Coli.

Appearance
A clear, colorless solution free from any particulate matter.

CHIKV E2

Chikungunya E2 Recombinant

Recombinant Chikungunya E2 protein, produced in E.coli, has a molecular weight of 38kDa. It appears as a band at approximately 38-40kDa on a 10% SDS-PAGE gel.
Shipped with Ice Packs
Cat. No.
BT264
Source
Escherichia Coli.
Appearance
A clear solution without any color, sterilized by filtration.

CHIKV Mutant

Chikungunya Mutant (A226V) E1 Recombinant

Recombinant Chikungunya Mutant (A226V) E1 protein, produced in insect cells, is a modified version of the chikungunya virus E1 protein. It encompasses amino acids 1 to 415, with a specific mutation where the Alanine at position 226 in the wild-type CHIKV E1 gene is replaced with Valine. This mutation distinguishes it from the naturally occurring E1 protein. The protein has a molecular weight of approximately 50kDa. It constitutes the C-terminal portion of the E2-6K-E1 protein region. Purification of the CHIKV Mutant protein is achieved using a proprietary chromatographic technique, ensuring its isolation and separation from other cellular components.
Shipped with Ice Packs
Cat. No.
BT343
Source
Insect cells.
Appearance
A clear, colorless solution that has been sterilized by filtration.

Chikungunya E1

Chikungunya Wild Type E1 Recombinant

Recombinant Chikungunya Wild Type E1, produced in insect cells, is a polypeptide chain encompassing amino acids 1 to 415. This protein has a molecular weight of approximately 50 kDa and is purified using a proprietary chromatographic technique.
Shipped with Ice Packs
Cat. No.
BT86
Source
Insect cells.
Appearance
A clear, colorless solution that has been sterilized by filtration.

CHIKV Capsid

Chikungunya Capsid Recombinant

This product consists of the Chikungunya virus capsid protein, produced in a laboratory setting using E. coli bacteria. The protein encompasses the full length of the native capsid protein, comprising 220 amino acids, and has a molecular weight of 25kDa.

Shipped with Ice Packs
Cat. No.
BT122
Source

Escherichia Coli.

Appearance
A clear and sterile solution without any color.
Definition and Classification

Chikungunya is a mosquito-borne viral disease caused by the chikungunya virus (CHIKV), an RNA virus in the alphavirus genus of the family Togaviridae . The name “chikungunya” is derived from a word in the Kimakonde language, meaning “to become contorted,” which refers to the stooped posture developed as a result of severe joint pain .

Biological Properties

Key Biological Properties: Chikungunya virus is a single-stranded positive-sense RNA virus with a genome of approximately 11.6 kb . It is closely related to other alphaviruses such as Ross River virus and O’nyong-nyong virus .

Expression Patterns and Tissue Distribution: CHIKV primarily targets fibroblasts, epithelial cells, endothelial cells, and macrophages . The virus replicates in the cytoplasm of infected cells and can be found in various tissues, including the skin, muscles, joints, and liver .

Biological Functions

Primary Biological Functions: The primary function of CHIKV is to replicate within the host cells and produce viral progeny. The virus induces a strong immune response, characterized by the production of cytokines and chemokines .

Role in Immune Responses and Pathogen Recognition: CHIKV infection triggers the activation of both innate and adaptive immune responses. The virus is recognized by pattern recognition receptors (PRRs) such as Toll-like receptors (TLRs) and RIG-I-like receptors (RLRs), leading to the production of type I interferons and other pro-inflammatory cytokines .

Modes of Action

Mechanisms with Other Molecules and Cells: CHIKV interacts with various host cell receptors to facilitate entry and replication. The virus binds to cell surface receptors, such as glycosaminoglycans, to gain entry into the host cell .

Binding Partners and Downstream Signaling Cascades: Once inside the host cell, CHIKV hijacks the cellular machinery to replicate its RNA genome and produce viral proteins. The virus activates several signaling pathways, including the NF-κB and MAPK pathways, to promote viral replication and suppress host immune responses .

Regulatory Mechanisms

Regulatory Mechanisms Controlling Expression and Activity: The expression and activity of CHIKV are regulated by various host factors. For example, the Musashi RNA binding protein-2 (MSI-2) is essential for efficient CHIKV genome replication .

Transcriptional Regulation and Post-Translational Modifications: CHIKV RNA is transcribed and replicated in the cytoplasm of infected cells. The virus undergoes several post-translational modifications, including glycosylation and phosphorylation, to enhance its stability and infectivity .

Applications

Biomedical Research: CHIKV is used as a model to study viral pathogenesis and host immune responses. Research on CHIKV has led to the development of potential antiviral therapies and vaccines .

Diagnostic Tools: Various diagnostic tools, including serological assays and molecular methods, are used to detect CHIKV infection .

Therapeutic Strategies: Although there is no specific antiviral treatment for chikungunya, supportive care and symptom relief are the standard treatments. Recent advances have led to the development of a vaccine approved by the U.S. FDA in 2023 .

Role in the Life Cycle

Role Throughout the Life Cycle: CHIKV plays a significant role in the life cycle of both the mosquito vector and the human host. The virus is transmitted to humans through the bite of infected Aedes mosquitoes, primarily Aedes aegypti and Aedes albopictus . In humans, CHIKV causes acute febrile illness and severe joint pain, which can persist for months or even years .

From Development to Aging and Disease: CHIKV infection can affect individuals of all ages, but the severity of the disease is often higher in infants, the elderly, and individuals with pre-existing health conditions . The virus can cause long-term health implications, including chronic joint pain and arthritis .

© Copyright 2024 Thebiotek. All Rights Reserved.