Zika Envelope F.Length

Zika Envelope Full Length Protein Recombinant

This product consists of the E. coli-derived recombinant Zika Envelope protein, specifically amino acids 40-400, with a molecular weight of 45 kDa. This represents the full-length Zika Envelope protein excluding the C-terminal hydrophobic region. This protein is fused with a 6xHis tag at its C-terminus and has been purified using a proprietary chromatographic technique.
Shipped with Ice Packs
Cat. No.
BT11033
Source
Escherichia Coli.
Appearance
Clear, sterile solution.

Zika Envelope N

Zika Envelope N-Terminal Recombinant

The Zika Envelope N protein is a peptide fragment derived from the N-terminal region of the Zika virus envelope protein. This 270-amino acid peptide has a molecular weight of 30 kDa and an isoelectric point of 6.37. To facilitate purification and detection, a 6xHis tag has been fused to the C-terminus of the Zika Envelope N protein. The protein is purified using a proprietary chromatographic technique, ensuring a high degree of purity.
Shipped with Ice Packs
Cat. No.
BT11130
Source
Escherichia Coli.
Appearance
Clear, sterile-filtered solution.

Zika Ectodomain

Zika Ectodomain Recombinant

Recombinant Zika Ectodomain, a glycosylated protein with an approximate molecular weight of 45 kDa, is produced through baculovirus expression in insect cells. This protein represents the ectodomain of the envelope protein derived from the Suriname Zika virus strain.
Shipped with Ice Packs
Cat. No.
BT10790
Source
Baculovirus Insect Cells.
Appearance
Sterile Filtered solution.

Zika Envelope

Zika Envelope Recombinant

This product consists of a recombinant Zika Envelope protein derived from E. coli, exhibiting a molecular weight of 19 kDa. The protein is engineered with a 6xHis tag fused to its C-terminus and undergoes purification using a proprietary chromatographic method.
Shipped with Ice Packs
Cat. No.
BT10824
Source
Escherichia Coli.
Appearance
Clear, sterile-filtered liquid.

Zika Envelope Domain-3

Zika Envelope Domain-III Recombinant

The Zika Envelope domain-III protein, derived from E. coli, is a non-glycosylated polypeptide chain with a molecular weight of 11 kDa. It is produced with a His tag fused to its N-terminus.
Shipped with Ice Packs
Cat. No.
BT10939
Source
Escherichia Coli.
Appearance
The product is a sterile, white powder that has been lyophilized (freeze-dried).

Zika Envelope, sf9

Zika Envelope Recombinant, sf9

Recombinant Zika Envelope protein derived from Sf9 cells has a molecular weight of approximately 50kDa. This protein is fused with a 6xHis tag for purification and is purified using a proprietary chromatographic technique.
Shipped with Ice Packs
Cat. No.
BT11221
Source
Sf9, Baculovirus cells.
Appearance
A sterile, filtered solution.

Zika NS1

Zika NS1 Protein Recombinant

The Zika NS1 protein is a recombinant protein derived from E. coli and based on the ZikaSPH2015 strain of the Zika virus. It has a molecular weight of 45 kDa and is produced from the full-length Zika NS1 protein sequence. For purification purposes, a 6xHis tag is attached to the C-terminus of the protein. The purification process involves proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT11286
Source
Escherichia Coli.
Appearance
Sterile solution that has been passed through a filter.

Zika NS1, HEK

Zika NS1 Protein Recombinant, HEK

This product features recombinant Zika NS1 protein derived from HEK cells. It originates from the MR-766 strain (Prototype, Uganda, 1947), with the GenBank accession number AY632535. The Zika NS1 protein has been engineered with a 6xHis tag fused to its C-terminus and has undergone purification using a proprietary chromatographic method.
Shipped with Ice Packs
Cat. No.
BT11367
Source
Human Embryonic Kidney 293 cells.
Appearance
Clear, sterile solution.

Zika NS1, sf9

Zika NS1 Protein Recombinant, sf9

This product consists of recombinant Zika NS1 protein produced in Sf9 insect cells. The protein is engineered with a 6xHis tag to facilitate purification, which is achieved using a proprietary chromatographic method.
Shipped with Ice Packs
Cat. No.
BT12016
Source
Sf9, Baculovirus cells.
Appearance
This product appears as a clear, sterile-filtered liquid.
Definition and Classification

The Zika virus (ZIKV) is a mosquito-borne flavivirus that belongs to the family Flaviviridae and the genus Flavivirus . It is closely related to other flaviviruses such as dengue, yellow fever, Japanese encephalitis, and West Nile viruses . ZIKV was first identified in a rhesus monkey in Uganda’s Zika Forest in 1947 and later in humans in 1952 .

Biological Properties

Key Biological Properties: ZIKV is an enveloped, icosahedral virus with a nonsegmented, single-stranded, positive-sense RNA genome of approximately 10.8 kilobases . The genome encodes a polyprotein that is cleaved into three structural proteins (capsid, envelope, and membrane) and seven nonstructural proteins .

Expression Patterns and Tissue Distribution: ZIKV primarily infects and replicates in various tissues, including the placenta and brain . It has been found in neural progenitor cells, astrocytes, and microglial cells .

Biological Functions

Primary Biological Functions: ZIKV’s primary function is to replicate within host cells and produce viral progeny. It plays a significant role in immune responses and pathogen recognition by interacting with host cell receptors and immune cells .

Role in Immune Responses and Pathogen Recognition: ZIKV infection triggers immune responses, including the production of neutralizing antibodies and activation of immune cells. The virus can evade immune detection by modulating host immune pathways .

Modes of Action

Mechanisms with Other Molecules and Cells: ZIKV interacts with host cell receptors, such as the envelope glycoprotein, to attach and enter human cells . It exploits cellular machinery for replication and assembly of new virions .

Binding Partners and Downstream Signaling Cascades: The virus binds to specific receptors on host cells, leading to endocytosis and release of viral RNA into the cytoplasm. This triggers downstream signaling cascades that facilitate viral replication and immune evasion .

Regulatory Mechanisms

Regulatory Mechanisms Controlling Expression and Activity: ZIKV infection activates the antioxidant nuclear factor erythroid 2-related factor 2 (Nrf2), which precedes oxidative stress . The virus also modulates host cell metabolism and homeostasis to promote its replication .

Transcriptional Regulation and Post-Translational Modifications: ZIKV relies on host cell machinery for transcription and translation of its genome. Post-translational modifications of viral proteins are essential for viral assembly and maturation .

Applications

Biomedical Research: ZIKV has become a focal point in biomedical research due to its association with severe congenital anomalies and neurological disorders . Research efforts aim to understand its pathogenesis, develop diagnostic tools, and identify therapeutic targets .

Diagnostic Tools: Diagnostic methods for ZIKV include molecular techniques such as RT-PCR to detect viral RNA and serological tests to identify antibodies .

Therapeutic Strategies: Current therapeutic strategies focus on developing antiviral drugs and vaccines to prevent ZIKV infection. Several candidate vaccines are in various stages of clinical trials .

Role in the Life Cycle

Role Throughout the Life Cycle: ZIKV is maintained in an enzootic cycle involving forest-dwelling Aedes mosquitoes and non-human primates . In humans, the virus can be transmitted through mosquito bites, sexual contact, and from mother to fetus . The virus’s ability to infect and replicate in various tissues, including the placenta and brain, contributes to its pathogenicity and impact on human health .

ZIKV’s life cycle involves attachment to host cell receptors, entry via endocytosis, replication of viral RNA, assembly of new virions, and release from the host cell . The virus’s dependence on host cell machinery for replication and its ability to evade immune responses are critical for its survival and propagation .

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