HCV Core 22kDa, Rhodamine

Hepatitis C Virus Core 22kDa, Rhodamine Recombinant

This recombinant HCV Core protein is derived from E. coli and encompasses amino acids 2-192, representing the immunodominant regions of the HCV nucleocapsid. The protein is labeled with Rhodamine and fused at its N-terminus to b-galactosidase (114 kDa).
Shipped with Ice Packs
Cat. No.
BT11438

HCV Core 24

Hepatitis C Virus Core 24 Recombinant

The HCV Core 24 protein is a recombinant protein derived from E. coli. It encompasses the immunodominant regions of the HCV core nucleocapsid of genotype 1b. This protein is expressed with an N-terminal fusion to b-galactosidase, resulting in a total molecular weight of 114 kDa.
Shipped with Ice Packs
Cat. No.
BT11546

HCV Core Genotype-1

Hepatitis C Virus Core Genotype-1 Recombinant

This recombinant HCV Core Genotype-1 protein is derived from E. coli and encompasses amino acids 1-102, representing the immunodominant regions of the HCV nucleocapsid.
Shipped with Ice Packs
Cat. No.
BT11619

HCV Core Genotype-1a

Hepatitis C Virus Core Genotype-1a Recombinant

This recombinant protein is derived from E. coli and contains amino acids 2-119 of the HCV core nucleocapsid immunodominant region. It is fused to a His tag at its C-terminus.
Shipped with Ice Packs
Cat. No.
BT11677

HCV Genotype 1b, 170 a.a.

Hepatitis C Virus Nucleocapsid (core) Genotype-1b, 170 a.a Recombinant

This product consists of the recombinant HCV Core protein derived from genotype 1b. It is produced in E. coli and encompasses 170 amino acids. The protein undergoes purification using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT12935
Source
Escherichia Coli.
Appearance
The product appears as a clear and colorless solution that has been sterilized by filtration.

HCV Mosaic-A

Hepatitis C Virus Mosaic Antigen-A Recombinant

HCV Mosaic-A protein is a recombinant protein engineered to include specific epitopes from the HCV virus. It comprises a short core peptide (residues 1-50), a fragment of the NS3 protein (residues 1269-1316), three epitopes from NS4, and two epitopes from NS5, all derived from the genotype 1b strain. This protein, with a molecular weight of 33 kDa, is produced in E. coli.
Shipped with Ice Packs
Cat. No.
BT13130
Source
Escherichia Coli.
Appearance
Clear, colorless, and sterile-filtered solution.

HCV NS3 Genotype-1a

Hepatitis C Virus NS3 Genotype-1a, (1192-1459 a.a.) Recombinant

This recombinant protein, derived from E. coli, encompasses the immunodominant regions of the HCV NS3 protein, specifically amino acids 1192-1459. It is expressed with a GST tag fused to its N-terminus.
Shipped with Ice Packs
Cat. No.
BT13961

HCV NS3 Genotype-1b

Hepatitis C Virus NS3 Genotype-1b Recombinant

The recombinant HCV NS3 protein, derived from E. coli, has a molecular weight of 26.2 kDa and encompasses the HCV NS3 c33c immunodominant regions. This 252-amino acid protein is fused with a 6xHis tag.
Shipped with Ice Packs
Cat. No.
BT14037

HCV NS3 Genotype-1b C33C

Hepatitis C Virus NS3 Genotype-1b C33C Recombinant

This product consists of a recombinant fragment of the Hepatitis C Virus NS3 protein, specifically from genotype 1b, spanning amino acids 40 to 315 (C33C region). The 226 amino acid protein was produced in E. coli and includes a 6xHis tag fused at the C-terminus. Purification is achieved using a proprietary chromatographic technique.

Shipped with Ice Packs
Cat. No.
BT14129
Source

Escherichia Coli.

Appearance

Sterile Filtered clear solution.

HCV NS3 Genotype-1c

Hepatitis C Virus NS3 Genotype-1c, (1192-1459 a.a.) Recombinant

This recombinant protein consists of the HCV NS3 immunodominant regions, spanning amino acids 1192 to 1459. It is produced in E. coli and includes a His-Tag for purification.
Shipped with Ice Packs
Cat. No.
BT14202
Definition and Classification

Hepatitis C is a liver disease caused by the hepatitis C virus (HCV), a small, enveloped, positive-sense single-stranded RNA virus belonging to the genus Hepacivirus within the family Flaviviridae . HCV can cause both acute and chronic hepatitis, ranging from a mild illness lasting a few weeks to a serious, lifelong illness including liver cirrhosis and cancer . There are seven major genotypes and numerous subtypes of HCV, which vary in their geographical distribution and response to treatment .

Biological Properties

HCV particles are spherical and range from 40 to 80 nanometers in diameter . The virus has a lipid membrane envelope embedded with two glycoproteins, E1 and E2, which play crucial roles in viral attachment and entry into host cells . HCV primarily infects hepatocytes in the liver but can also be found in other tissues such as lymph nodes and peripheral blood mononuclear cells . The virus exhibits significant genetic diversity, which contributes to its ability to evade the host immune system .

Biological Functions

HCV’s primary function is to replicate within host cells. It hijacks the host’s cellular machinery to produce viral proteins and RNA . The virus plays a role in immune evasion by interfering with the host’s immune responses, including the inhibition of interferon signaling pathways . HCV also induces chronic inflammation, which can lead to liver fibrosis, cirrhosis, and hepatocellular carcinoma .

Modes of Action

HCV enters host cells through interactions with several receptors, including the low-density lipoprotein receptor (LDLr), heparan sulfate proteoglycans (HSPGs), scavenger receptor B1 (SRB1), and CD81 . Once inside the cell, the virus releases its RNA genome, which is translated into a single polyprotein. This polyprotein is then cleaved into structural and non-structural proteins that are essential for viral replication . The virus also manipulates host cell signaling pathways to create a favorable environment for its replication .

Regulatory Mechanisms

The expression and activity of HCV are regulated at multiple levels. Transcriptional regulation involves the interaction of viral RNA with host cell factors that enhance or inhibit viral replication . Post-translational modifications, such as phosphorylation and ubiquitination, also play critical roles in regulating the stability and function of viral proteins . Additionally, HCV can modulate host immune responses to promote viral persistence .

Applications in Biomedical Research

HCV research has led to significant advancements in understanding viral pathogenesis and developing therapeutic strategies. Direct-acting antivirals (DAAs) have revolutionized HCV treatment, offering high cure rates with fewer side effects compared to previous therapies . HCV is also used as a model to study RNA virus replication and host-virus interactions . Diagnostic tools, such as serological assays and nucleic acid tests, are essential for detecting HCV infection and monitoring treatment response .

Role in the Life Cycle

HCV’s life cycle begins with the attachment of the virus to host cell receptors, followed by entry into the cell via endocytosis . Once inside, the viral RNA is released and translated into a polyprotein, which is processed into functional viral proteins . These proteins facilitate viral RNA replication and assembly of new virions, which are then released to infect other cells . Throughout its life cycle, HCV interacts with various host factors to ensure its replication and persistence .

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