Dengue Envelope-1 & 3

Dengue Virus Subtype 1 & 3 fused Envelope 58kDa Recombinant

This recombinant protein is derived from E. coli and consists of a genetically engineered peptide representing a fusion of the envelope proteins from Dengue Types 1 and 3. Each part of the fusion contains 170 amino acids (positions 46-217) and is intended for use in ELISA assays. The fusion protein is tagged with a 6xHis tag. Dengue Types 1 and 3 are purified using a proprietary chromatographic method.
Shipped with Ice Packs
Cat. No.
BT1981
Source
Escherichia Coli.
Appearance
A sterile, colorless solution.

Dengue Envelope-1 & 4

Dengue Virus Subtype 1 & 4 fused Envelope 55kDa Recombinant

This product is a recombinant protein engineered from Dengue Virus serotypes 1 and 4. It consists of a 55kDa fusion protein containing amino acids 46-217 from the envelope protein of each serotype, totaling 340 amino acids. Produced in E. coli, it is purified using a proprietary chromatographic technique and is designed for use in ELISA assays. The protein is attached to a 6xHis Tag for ease of purification and detection.
Shipped with Ice Packs
Cat. No.
BT2056
Source
Escherichia Coli.
Appearance
A clear, colorless liquid free from any visible particles.

Dengue Envelope-1 15kDa

Dengue Virus Subtype 1 Envelope 15kDa, C-Terminal (Domain III) Recombinant

This product consists of a recombinant 15kDa protein derived from E. coli. It is a genetically engineered peptide representing the C terminus of the Dengue Type-1 envelope protein. This protein includes both neutralizing epitopes and the receptor binding domain, making it a valuable tool in vaccine development research. For purification purposes, a 6x His Tag has been fused to the protein's C-terminus.
Shipped with Ice Packs
Cat. No.
BT2130
Source
Escherichia Coli.

Dengue Envelope-3 32kDa

Dengue Virus Subtype 3 Envelope 32kDa Recombinant

Recombinant Dengue Virus Subtype 3 Envelope protein (32kDa) is produced in E.coli. It includes a 6xHis Tag and encompasses domains I and II of the dengue envelope.
Shipped with Ice Packs
Cat. No.
BT3551
Source
E.coli.

Dengue NS1 ST2

Dengue Virus NS1 Subtype 2 Recombinant

Recombinant Dengue Virus NS1 Subtype 2, expressed in insect cells, is a polypeptide chain encompassing amino acids 777-1131 of the Dengue virus NS1 protein. This protein fragment has an approximate molecular weight of 50kDa. It includes a His tag for purification and is purified using a proprietary chromatographic method.
Shipped with Ice Packs
Cat. No.
BT4720
Source
Insect cells.
Appearance
A clear, colorless solution that has been sterilized by filtration.

Dengue NS1 ST3, Insect

Dengue Virus NS1 Subtype 3 Recombinant, Insect Cells

Recombinant Dengue Virus NS1 Subtype 3, expressed in insect cells, is a polypeptide chain encompassing amino acids 775-1129. It possesses a molecular weight of approximately 50kDa. The purification of Dengue NS1 ST3 is achieved through a proprietary chromatographic method.
Shipped with Ice Packs
Cat. No.
BT4799
Source
Insect cells.
Appearance
A clear, colorless solution that has been sterilized by filtration.

Dengue NS1 ST4, Insect

Dengue Virus NS1 Subtype 4 Recombinant, Insect Cells

Recombinant Dengue Virus NS1 Subtype 4, produced in insect cells, is a polypeptide chain encompassing amino acids 776-1130. It has a molecular weight of approximately 50kDa. The purification of Dengue NS1 ST4 is carried out using a proprietary chromatographic technique.
Shipped with Ice Packs
Cat. No.
BT4890
Source
Insect cells.
Appearance
A clear, colorless solution that has been sterilized by filtration.

Dengue NS1, ST1

Dengue Virus NS1 Recombinant, Subtype-1

The E.Coli derived recombinant protein contains the NS1 Dengue Virus full length Type-1 immunodominant regions and migrates as 45kDa on 12% SDS-PAGE gel. The dengue protein is fused to a 6xHis tag at the C-terminus.
Shipped with Ice Packs
Cat. No.
BT4997
Source
Escherichia Coli.

Dengue Envelope-1 22kDa

Dengue Virus Subtype-1 Envelope 22kDa Recombinant

This recombinant 22 kDa protein is derived from E. coli and represents a genetically engineered peptide fragment from the N-terminus Envelope immunodeterminant region of Dengue virus Type-1. A 6 amino acid His Tag is fused to the peptide. Notably, this region contains an antigen common to Dengue virus types 1, 2, and 3.
Shipped with Ice Packs
Cat. No.
BT2195

Dengue Envelope-2 & 4

Dengue Virus Subtype 2 & 4 fused Envelope 52kDa Recombinant

This product consists of a recombinant 52kDa protein derived from E. coli. This genetically engineered protein is a fusion of the envelope proteins of Dengue virus types 2 and 4. Each component of the fusion contains 170 amino acids (positions 46-217) and is intended for use in ELISA assays. The fusion protein also incorporates a 6xHis Tag for ease of purification. Purification of the Dengue Type-2 and 4 proteins is achieved through a proprietary chromatographic technique.
Shipped with Ice Packs
Cat. No.
BT2578
Source
Escherichia Coli.
Appearance
A clear, colorless liquid that has been sterilized by filtration.
Definition and Classification

Dengue is a mosquito-borne viral infection caused by the dengue virus (DENV), which belongs to the Flavivirus genus of the Flaviviridae family . There are four distinct but closely related serotypes of the virus: DEN-1, DEN-2, DEN-3, and DEN-4 . Dengue is classified into three categories: undifferentiated fever, dengue fever (DF), and dengue hemorrhagic fever (DHF) .

Biological Properties

The dengue virus is a single positive-stranded RNA virus . It has four serotypes, each with different interactions with antibodies in human blood serum . The virus is primarily transmitted by the Aedes aegypti mosquito, which is active during the day . The virus’s key biological properties include its ability to infect a variety of cell types, including immune cells, and its expression patterns that vary depending on the host’s immune response .

Biological Functions

The primary biological function of the dengue virus is to replicate within the host cells. It plays a significant role in immune responses and pathogen recognition. The virus tricks the immune system to evade its defenses and infect more cells . The host’s immune response includes the production of antibodies and activation of T cells, which can sometimes lead to severe immune reactions .

Modes of Action

Dengue virus infects cells by binding to receptors on the host cell’s surface and entering through endocytosis . Once inside, the virus releases its RNA into the cytoplasm, where it is translated into viral proteins . These proteins form a replication complex that produces new viral RNA and proteins, which assemble into new virus particles . The virus also interacts with other molecules and cells, leading to the release of cytokines and other immune responses .

Regulatory Mechanisms

The expression and activity of the dengue virus are regulated at multiple levels. Epigenetic modulation of the host genome, transcription of host genes, translation of viral and host mRNAs, post-transcriptional regulation of the host transcriptome, and post-translational modifications of viral proteins all play a role in the virus’s life cycle . These regulatory mechanisms help the virus evade the host’s immune system and ensure efficient replication .

Applications in Biomedical Research

Dengue virus research has led to significant advancements in diagnostic tools and therapeutic strategies. Diagnostic tools include polymerase chain reaction (PCR) tests to detect viral RNA and serological tests to detect antibodies . Therapeutic strategies focus on supportive care, as there is no specific antiviral treatment for dengue . Research is ongoing to develop effective vaccines and antiviral drugs . The introduction of Wolbachia-infected mosquitoes and gene-editing technologies are also being explored as potential control strategies .

Role in the Life Cycle

The dengue virus plays a crucial role throughout its life cycle, from development to aging and disease. It infects immune system cells, leading to a range of symptoms from mild fever to severe hemorrhagic fever . The virus’s ability to evade the immune system and replicate efficiently within host cells is key to its survival and transmission . Understanding the virus’s life cycle is essential for developing effective control and treatment strategies .

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