Product List

ASFV P30

African Swine Fever Virus P30

Recombinant African Swine Fever Virus P30 produced in E. coli containing 194 amino acids (40-315 aa) and having a Mw of 22kDa.
Recombinant African Swine Fever Virus P30 is fused to a 6xHis tag and purified by proprietary chromatographic technique.

Shipped with Ice Packs
Cat. No.
BT28007
Source

Escherichia Coli.

Appearance
Sterile Filtered colorless solution.

ASFV P72

African Swine Fever Virus P72 Recombinant

Recombinant African Swine Fever Virus P72 (40-315 aa) produced in E.coli having a Mw of 33kDa.
The African Swine Fever Virus P72 is fused to a 6xHis tag and purified by proprietary chromatographic technique.

Shipped with Ice Packs
Cat. No.
BT28098
Source

Escherichia Coli.

Appearance
Sterile Filtered colorless solution.

Introduction

Definition and Classification

African Swine Fever Virus (ASFV) is a large, double-stranded DNA virus belonging to the family Asfarviridae and the genus Asfivirus . ASFV is the causative agent of African swine fever (ASF), a highly contagious and often lethal hemorrhagic disease affecting domestic pigs and wild boars . ASFV is the only known DNA virus transmitted by arthropods .

Biological Properties

Key Biological Properties: ASFV is a large, enveloped virus with a complex structure, including an icosahedral capsid, inner membrane, and core shell . The virus has a linear genome of approximately 170-190 kilobases, encoding more than 150 proteins .

Expression Patterns and Tissue Distribution: ASFV primarily infects cells of the monocyte-macrophage lineage . The virus replicates in the cytoplasm of infected cells, forming discrete perinuclear factory areas . ASFV is found in high concentrations in various tissues, including the tonsils, lymph nodes, spleen, and liver .

Biological Functions

Primary Biological Functions: ASFV’s primary function is to replicate within host cells and produce progeny virions . The virus encodes numerous proteins that modulate host immune responses, inhibit apoptosis, and facilitate viral replication .

Role in Immune Responses and Pathogen Recognition: ASFV evades the host immune system by inhibiting the activation of cytokines such as interferon (IFN) and tumor necrosis factor-alpha (TNF-α) . The virus also encodes proteins that interfere with host gene transcription and immune signaling pathways .

Modes of Action

Mechanisms with Other Molecules and Cells: ASFV enters host cells through receptor-mediated endocytosis, utilizing pathways such as clathrin-dependent endocytosis and macropinocytosis . The virus encodes proteins that inhibit signaling pathways in infected macrophages, modulating transcriptional activation of immune response genes .

Binding Partners and Downstream Signaling Cascades: ASFV proteins interact with various host cell receptors and signaling molecules to facilitate viral entry, replication, and immune evasion . The virus also encodes enzymes required for replication and transcription of its genome .

Regulatory Mechanisms

Regulatory Mechanisms Controlling Expression and Activity: ASFV regulates its gene expression through a highly orchestrated process involving immediate-early, early, intermediate, and late transcription stages . The virus encodes proteins that modulate host cell signaling pathways and immune responses .

Transcriptional Regulation and Post-Translational Modifications: ASFV utilizes various transcriptional regulators and post-translational modifications to control the expression and activity of its proteins . The virus also encodes proteins that inhibit apoptosis and promote viral replication .

Applications

Biomedical Research: ASFV serves as a model for studying viral pathogenesis, immune evasion, and host-virus interactions . Researchers are exploring the use of CRISPR technology to develop attenuated virus vaccines for ASFV .

Diagnostic Tools and Therapeutic Strategies: ASFV detection relies on molecular techniques such as PCR and serological assays . Efforts are ongoing to develop effective vaccines and antiviral drugs to control ASFV infections .

Role in the Life Cycle

Role Throughout the Life Cycle: ASFV plays a critical role in the life cycle of infected pigs, from initial infection to disease progression and transmission . The virus undergoes various stages of replication, assembly, and release within host cells . ASFV’s ability to evade the host immune system and persist in the environment contributes to its high transmissibility and pathogenicity .

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