Monkeypox A33R

Monkeypox A33R Recombinant

The recombinant Monkeypox A33R protein, derived from E. coli, encompasses the immunodominant regions of the Monkeypox virus envelope. With a molecular weight of 23kDa, the protein is fused with a 6xHis tag at the C-terminus and purified using a proprietary chromatographic method.

Shipped with Ice Packs
Cat. No.
BT633
Source

Escherichia Coli.

Appearance

The product appears as a clear, sterile-filtered solution.

Monkeypox B5R

Monkeypox B5R Recombinant

The recombinant B5R protein, derived from E. coli, encompasses the immunodominant regions of the Monkeypox virus envelope protein and has a molecular weight of 32 kDa. It is tagged with a C-terminal 6xHis tag and purified using a proprietary chromatographic method.

Shipped with Ice Packs
Cat. No.
BT716
Source

Escherichia Coli.

Appearance

A clear, sterile-filtered solution.

Definition and Classification

Monkeypox, now referred to as Mpox, is a viral illness caused by the monkeypox virus (MPXV), a species of the genus Orthopoxvirus in the Poxviridae family . There are two distinct clades of the virus: Clade I (with subclades Ia and Ib) and Clade II (with subclades IIa and IIb) . The virus is zoonotic, meaning it can be transmitted from animals to humans .

Biological Properties

Key Biological Properties: The monkeypox virus is an enveloped double-stranded DNA virus . It is relatively large, with viral particles measuring approximately 200-250 nm in diameter .

Expression Patterns and Tissue Distribution: The virus primarily infects skin cells, leading to the characteristic rash and lesions associated with the disease . It can also be found in bodily fluids, respiratory droplets, and contaminated objects .

Biological Functions

Primary Biological Functions: The monkeypox virus is responsible for causing the disease Mpox, which presents with symptoms similar to smallpox but generally less severe . The virus triggers an immune response in the host, leading to the production of antibodies and activation of T-cells .

Role in Immune Responses and Pathogen Recognition: The virus utilizes virotransducer and virostealth proteins to control the host’s T-cell-mediated responses and impede the release of cytokines and chemokines . This helps the virus evade the host’s immune system and establish infection .

Modes of Action

Mechanisms with Other Molecules and Cells: The monkeypox virus can infiltrate host cells through membrane fusion and endocytosis . Once inside, it replicates in the cytoplasm and produces viral proteins that interfere with the host’s immune response .

Binding Partners and Downstream Signaling Cascades: The virus binds to specific receptors on the host cell surface, initiating a cascade of signaling events that facilitate viral entry and replication . This process involves various viral and host proteins that modulate the immune response and promote viral survival .

Regulatory Mechanisms

Transcriptional Regulation: The expression of viral genes is tightly regulated to ensure efficient replication and evasion of the host immune system . The virus uses host cell machinery to transcribe its DNA and produce viral proteins .

Post-Translational Modifications: Viral proteins undergo post-translational modifications to enhance their stability and functionality . These modifications are crucial for the virus to effectively replicate and evade the host immune response .

Applications in Biomedical Research

Diagnostic Tools: Detection of viral DNA by polymerase chain reaction (PCR) is the preferred method for diagnosing Mpox . This technique allows for the rapid and accurate identification of the virus in clinical samples .

Therapeutic Strategies: Antiviral compounds such as tecovirimat, cidofovir, and brincidofovir have shown efficacy against the monkeypox virus . Vaccination with the JYNNEOS vaccine is also recommended for prevention .

Role in the Life Cycle

Role Throughout the Life Cycle: The monkeypox virus plays a critical role in the life cycle of its host by establishing infection, evading the immune response, and facilitating transmission to new hosts . The virus can be transmitted through close contact with infected individuals, contaminated objects, and respiratory droplets . It can also be passed from mother to fetus during pregnancy .

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