Viral Antigens
Product List
CoV-2 Envelope (1-75)
Coronavirus 2019 Envelope (1-75 a.a.), Recombinant
This product consists of a recombinant protein derived from E. coli. It comprises the Envelope protein of the Coronavirus 2019 (CoV-2), specifically the Wuhan-Hu-1 strain, encompassing amino acids 1 to 75. The protein is fused with a GST-tag at the N-terminal and a His-tag at the C-terminal. Its calculated molecular weight is 36.8 kDa, and under reducing conditions, it migrates between 33-35 kDa on SDS-PAGE.
E.Coli
CoV-2 Membrane Env.
Coronavirus 2019 Membrane Envelope, Recombinant
This recombinant fusion protein is produced in E. coli and consists of the full-length Membrane (M) and Envelope (E) proteins of the Wuhan-Hu-1 strain of Coronavirus 2019. The protein has a molecular weight of 34.2 kDa and includes a C-terminal His tag for purification purposes.
E.Coli
CoV-2 N (127 a.a.)
Coronavirus 2019 Nucleocapsid (127 a.a.), Recombinant
This recombinant protein, derived from E. coli, encompasses the C-terminal 127 amino acids of the Coronavirus 2019 Nucleocapsid protein. It is fused to a GST-6xHis tag at the N-terminal and has a molecular weight of 39.4 kDa.
E.Coli.
The product is a clear solution that has undergone sterile filtration.
CoV-2 N (1-419)
Coronavirus 2019 Nucleocapsid (1-419 a.a.), Recombinant
This recombinant protein is derived from E. coli and represents the nucleocapsid phosphoprotein of the Wuhan-Hu-1 strain of Coronavirus 2019 (CoV-2). It encompasses amino acids 1-419 and has a molecular weight of 46.4 kDa, including a C-terminal 6xHis tag.
E.Coli
CoV-2 N (1-419), Biotin
Coronavirus 2019 Nucleocapsid (1-419 a.a.), Biotinylated Recombinant
This recombinant protein consists of the nucleocapsid protein (amino acids 1-419) of SARS-CoV-2 (Wuhan-Hu-1 strain), expressed in HEK293 cells. It is biotinylated and features a C-terminal His-Avi tag for purification and detection purposes.
HEK293 Cells.
CoV-2 N (1-419), HEK
Coronavirus 2019 Nucleocapsid (1-419 a.a.), Recombinant, HEK
This recombinant protein is derived from HEK293 cells and encompasses amino acids 1 to 419 of the SARS-CoV-2 Nucleocapsid protein (Wuhan-Hu-1 strain). A C-terminal His tag is present for purification purposes. The calculated molecular weight is 47.3 kDa. However, due to glycosylation, the protein exhibits a migration pattern between 60-65 kDa on SDS-PAGE.
HEK293 Cells.
CoV-2 N (196 a.a.)
Coronavirus 2019 Nucleocapsid (196 a.a.), Recombinant
This E. coli-derived recombinant protein consists of the middle region (amino acids 196) of the Coronavirus 2019 Nucleocapsid protein. It is fused to a GST-6xHis tag at the N-terminus and has a molecular weight of 48.4 kDa.
E.Coli.
The product is a clear, sterile-filtered solution.
ACE Human
Angiotensin Converting Enzyme Human Recombinant
Sf9, Baculovirus cells.
CoV-2 S2
Novel Coronavirus 2019-nCoV Spike Glycoprotein-S2, Recombinant
This recombinant protein, produced in HEK293 cells, encompasses the S2 subunit (amino acids 685-1211) of the Spike Glycoprotein from the Wuhan-Hu-1 strain of the 2019 Novel Coronavirus (2019-nCoV). It also incorporates an Fc tag fused to its C-terminal end.
HEK293
CoV-2 S2, Sf9
Coronavirus 2019 Spike Glycoprotein-S2, Sf9 Recombinant
This recombinant protein, expressed in Sf9 insect cells, encompasses the S2 subunit of the Spike Glycoprotein from the Wuhan-Hu-1 strain of the Coronavirus 2019 (CoV-2). It spans amino acids 685 to 1211, resulting in a molecular weight of 60.1 kDa, and includes a C-terminal 6xHis tag for purification purposes.
Sf9, Baculovirus Cells.
Introduction
Severe Acute Respiratory Syndrome (SARS) is a viral respiratory illness caused by a coronavirus known as SARS-CoV. It was first identified in 2003 during an outbreak that began in China and spread to other countries . SARS-CoV belongs to the family Coronaviridae, which is divided into four genera: Alpha, Beta, Gamma, and Delta coronaviruses . SARS-CoV is classified under the Betacoronavirus genus.
Key Biological Properties: SARS-CoV is an enveloped, positive-sense, single-stranded RNA virus. It has a crown-like appearance due to spike proteins on its surface .
Expression Patterns and Tissue Distribution: SARS-CoV primarily infects the respiratory tract, but it can also affect other organs such as the gastrointestinal tract, liver, and kidneys . The virus binds to the angiotensin-converting enzyme 2 (ACE2) receptor, which is widely distributed in various tissues, including the lungs, heart, and intestines .
Primary Biological Functions: The primary function of SARS-CoV is to replicate within host cells. The virus hijacks the host’s cellular machinery to produce viral RNA and proteins, leading to the assembly of new virions .
Role in Immune Responses and Pathogen Recognition: SARS-CoV triggers an immune response by activating various immune cells and signaling pathways. The spike protein of the virus is recognized by the host’s immune system, leading to the production of neutralizing antibodies .
Mechanisms with Other Molecules and Cells: SARS-CoV enters host cells by binding to the ACE2 receptor and undergoing proteolytic cleavage by host cell proteases such as TMPRSS2 . This facilitates viral entry and fusion with the host cell membrane.
Binding Partners and Downstream Signaling Cascades: The binding of SARS-CoV to ACE2 triggers downstream signaling cascades that modulate immune responses and inflammation. The virus can also evade immune detection by interfering with interferon signaling pathways .
Transcriptional Regulation: The expression of SARS-CoV genes is tightly regulated by viral and host factors. Transcription factors such as SP1 and HNF4α play crucial roles in regulating the expression of the ACE2 receptor, which is essential for viral entry .
Post-Translational Modifications: SARS-CoV proteins undergo various post-translational modifications, including phosphorylation, glycosylation, and ubiquitination, which are critical for viral replication and immune evasion .
Biomedical Research: SARS-CoV has been extensively studied to understand viral pathogenesis and host immune responses. This research has led to the development of diagnostic tools and therapeutic strategies .
Diagnostic Tools: Techniques such as polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) are used to detect SARS-CoV infection .
Therapeutic Strategies: Antiviral drugs, monoclonal antibodies, and vaccines have been developed to combat SARS-CoV infection. These therapeutic strategies target various stages of the viral life cycle .
Throughout the Life Cycle: SARS-CoV plays a critical role in the viral life cycle, from initial infection to replication and assembly of new virions. The virus hijacks the host’s cellular machinery to produce viral RNA and proteins, leading to the assembly of new virions . The N protein of SARS-CoV is essential for packaging the viral RNA into new virions and facilitating their release from host cells .
