Viral Antigens
Product List
SARS Core (340-390)
SARS-Associated Coronavirus Nucleocapsid Core Recombinant, (340-390 a.a)
Escherichia Coli.
SARS Envelope
SARS-Associated Coronavirus Envelope Recombinant
SARS Envelope, His
SARS-Associated Coronavirus Envelope Recombinant, His Tag
This recombinant protein is derived from E. coli and consists of the Envelope protein's immunodominant region, spanning amino acids 1-76, fused to a C-terminal 6xHis tag.
SARS Matrix
SARS-Associated Coronavirus Matrix Recombinant
SARS Membrane
SARS-Associated Coronavirus Membrane (Matrix), Recombinant
This recombinant protein is derived from E. coli bacteria and contains the immunodominant regions of the SARS Membrane/Matrix protein. It has a 6xHis tag fused to its C-terminal end.
SARS Mosaic S(M)
SARS-Associated Coronavirus Spike Mosaic S (M) Recombinant
SARS Mosaic S(N)
SARS-Associated Coronavirus Spike Mosaic S (N) Recombinant
SARS Nucleocapsid (1-49)
SARS-Associated Coronavirus Nucleocapsid Core Recombinant, 1-49 a.a.
SARS Nucleocapsid (340-390), His
SARS-Associated Coronavirus Nuclecapsid (340-390 a.a.) Recombinant, His Tag
This recombinant protein is derived from E. coli and comprises the C-terminal region (amino acids 340-390) of the SARS Coronavirus Nucleocapsid protein. This region, known for its immunodominant properties, is expressed with a 6xHis tag fused to its C-terminus.
SARS-CoV
SARS-CoV Nucleocapsid (422a.a) Recombinant
The Recombinant SARS-CoV Nucleocapsid Protein is engineered with an N-terminal His Tag fusion. This His-Tagged Fusion Protein boasts a molecular weight of 47.8kDa, encompassing 437 amino acid residues of the SARS-CoV Nucleocapsid protein alongside 16 additional amino acid residues constituting the His Tag (underlined).
Introduction
Severe Acute Respiratory Syndrome (SARS) is a viral respiratory illness caused by a coronavirus known as SARS-CoV. It was first identified in 2003 during an outbreak that began in China and spread to other countries . SARS-CoV belongs to the family Coronaviridae, which is divided into four genera: Alpha, Beta, Gamma, and Delta coronaviruses . SARS-CoV is classified under the Betacoronavirus genus.
Key Biological Properties: SARS-CoV is an enveloped, positive-sense, single-stranded RNA virus. It has a crown-like appearance due to spike proteins on its surface .
Expression Patterns and Tissue Distribution: SARS-CoV primarily infects the respiratory tract, but it can also affect other organs such as the gastrointestinal tract, liver, and kidneys . The virus binds to the angiotensin-converting enzyme 2 (ACE2) receptor, which is widely distributed in various tissues, including the lungs, heart, and intestines .
Primary Biological Functions: The primary function of SARS-CoV is to replicate within host cells. The virus hijacks the host’s cellular machinery to produce viral RNA and proteins, leading to the assembly of new virions .
Role in Immune Responses and Pathogen Recognition: SARS-CoV triggers an immune response by activating various immune cells and signaling pathways. The spike protein of the virus is recognized by the host’s immune system, leading to the production of neutralizing antibodies .
Mechanisms with Other Molecules and Cells: SARS-CoV enters host cells by binding to the ACE2 receptor and undergoing proteolytic cleavage by host cell proteases such as TMPRSS2 . This facilitates viral entry and fusion with the host cell membrane.
Binding Partners and Downstream Signaling Cascades: The binding of SARS-CoV to ACE2 triggers downstream signaling cascades that modulate immune responses and inflammation. The virus can also evade immune detection by interfering with interferon signaling pathways .
Transcriptional Regulation: The expression of SARS-CoV genes is tightly regulated by viral and host factors. Transcription factors such as SP1 and HNF4α play crucial roles in regulating the expression of the ACE2 receptor, which is essential for viral entry .
Post-Translational Modifications: SARS-CoV proteins undergo various post-translational modifications, including phosphorylation, glycosylation, and ubiquitination, which are critical for viral replication and immune evasion .
Biomedical Research: SARS-CoV has been extensively studied to understand viral pathogenesis and host immune responses. This research has led to the development of diagnostic tools and therapeutic strategies .
Diagnostic Tools: Techniques such as polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) are used to detect SARS-CoV infection .
Therapeutic Strategies: Antiviral drugs, monoclonal antibodies, and vaccines have been developed to combat SARS-CoV infection. These therapeutic strategies target various stages of the viral life cycle .
Throughout the Life Cycle: SARS-CoV plays a critical role in the viral life cycle, from initial infection to replication and assembly of new virions. The virus hijacks the host’s cellular machinery to produce viral RNA and proteins, leading to the assembly of new virions . The N protein of SARS-CoV is essential for packaging the viral RNA into new virions and facilitating their release from host cells .
