Viral Antigens
Product List
CoV-2 Spike (1000-1200)
Coronavirus 2019 Spike (1000-1200 a.a.) Recombinant
This recombinant protein, produced in E. coli, encompasses the immunodominant regions of the Coronavirus 2019 Spike protein (amino acids 1000-1200). It is equipped with a C-terminal 6xHis tag for purification and detection purposes.
CoV-2 Spike (300-600)
Coronavirus 2019 Spike Receptor Binding Domain (300-600 a.a.) Recombinant
This product consists of the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein (amino acids 300-600), produced in E. coli. This immunodominant region is fused to a C-terminal 6xHis tag for purification and detection purposes.
CoV-2 Spike (318-542)
Coronavirus 2019 Spike Receptor Binding Domain (318-542 a.a.) Recombinant
This product consists of the recombinant Coronavirus 2019 Spike Receptor Binding Domain (amino acids 318-542), with a molecular weight of 25.7 kDa. Expressed in E. coli, this protein features a C-terminal 6xHis tag and undergoes purification via a proprietary chromatographic method.
CoV-2 S1 (319-529)
Coronavirus 2019-nCoV Spike Glycoprotein-S1 Receptor Binding Domain (319-529 a.a.) Recombinant
CoV-2 S1 (319-537)
Coronavirus 2019 Spike Glycoprotein-S1 Receptor Binding Domain (319-537 a.a), Recombinant
This recombinant protein, derived from HEK293 cells, corresponds to the Receptor Binding Domain (RBD) of the SARS-CoV-2 Spike Glycoprotein S1 (Wuhan-Hu-1 strain, amino acids 319-537). This protein, with a molecular weight of 26.5kDa, includes a C-terminal His tag for purification and detection.
HEK293 Cells.
CoV-2 S1 (319-541), Sf9
Coronavirus 2019-nCoV Spike Glycoprotein-S1 Receptor Binding Domain Recombinant,SF9
This product consists of a single, glycosylated polypeptide chain representing the Receptor Binding Domain (RBD) of the SARS-CoV-2 Spike Glycoprotein-S1. It encompasses amino acids 319-541, resulting in a molecular weight of 26.2 kDa.
A 6 amino acid His-tag is fused to the C-terminus of the CoV-2 S1 (319-541). Purification is achieved using proprietary chromatographic techniques.
Sf9, Baculovirus cells.
CoV-2 S1, Sf9
Coronavirus 2019 Spike Glycoprotein-S1 Sf9, Recombinant
This recombinant protein, expressed in Sf9 insect cells, consists of the Spike Glycoprotein S1 subunit (amino acids 1-674) from the Wuhan-Hu-1 strain of the 2019 Coronavirus (CoV-2). It also includes a C-terminal His tag for purification.
Sf9, Baculovirus Cells.
CoV-2-S1
Coronavirus 2019 Spike Glycoprotein-S1, Recombinant
This recombinant protein consists of the Spike Glycoprotein S1 subunit (amino acids 1-674) of the 2019 Novel Coronavirus (2019-nCoV), specifically the Wuhan-Hu-1 strain. It is produced in HEK293 cells and is fused with an Fc tag at its C-terminus.
HEK293
CoV-2-S1 (319-541)
Coronavirus 2019 Spike Glycoprotein-S1 Receptor Binding Domain (319-541 a.a), Recombinant
This recombinant protein is derived from HEK293 cells and encompasses the Receptor Binding Domain (RBD) of the SARS-CoV-2 Spike Glycoprotein S1, specifically the Wuhan-Hu-1 strain (amino acids 319-541). It is expressed with a C-terminal His tag for purification and detection purposes.
HEK293 Cells.
CoV-2-S1 (319-541), Biotin
Coronavirus 2019 Spike Glycoprotein-S1 Receptor Binding Domain (319-541 a.a), Biotinylated Recombinant
This product is a biotinylated recombinant protein derived from HEK293 cells. It consists of the Receptor Binding Domain (RBD) of the Coronavirus 2019 Spike Glycoprotein S1, specifically the Wuhan-Hu-1 strain, encompassing amino acids 319-541. A His tag and an AVI tag are fused to the C-terminal. The protein has a molecular mass of 28.7kDa.
HEK293 Cells.
The product is supplied as a lyophilized, freeze-dried powder.
Introduction
Severe Acute Respiratory Syndrome (SARS) is a viral respiratory illness caused by a coronavirus known as SARS-CoV. It was first identified in 2003 during an outbreak that began in China and spread to other countries . SARS-CoV belongs to the family Coronaviridae, which is divided into four genera: Alpha, Beta, Gamma, and Delta coronaviruses . SARS-CoV is classified under the Betacoronavirus genus.
Key Biological Properties: SARS-CoV is an enveloped, positive-sense, single-stranded RNA virus. It has a crown-like appearance due to spike proteins on its surface .
Expression Patterns and Tissue Distribution: SARS-CoV primarily infects the respiratory tract, but it can also affect other organs such as the gastrointestinal tract, liver, and kidneys . The virus binds to the angiotensin-converting enzyme 2 (ACE2) receptor, which is widely distributed in various tissues, including the lungs, heart, and intestines .
Primary Biological Functions: The primary function of SARS-CoV is to replicate within host cells. The virus hijacks the host’s cellular machinery to produce viral RNA and proteins, leading to the assembly of new virions .
Role in Immune Responses and Pathogen Recognition: SARS-CoV triggers an immune response by activating various immune cells and signaling pathways. The spike protein of the virus is recognized by the host’s immune system, leading to the production of neutralizing antibodies .
Mechanisms with Other Molecules and Cells: SARS-CoV enters host cells by binding to the ACE2 receptor and undergoing proteolytic cleavage by host cell proteases such as TMPRSS2 . This facilitates viral entry and fusion with the host cell membrane.
Binding Partners and Downstream Signaling Cascades: The binding of SARS-CoV to ACE2 triggers downstream signaling cascades that modulate immune responses and inflammation. The virus can also evade immune detection by interfering with interferon signaling pathways .
Transcriptional Regulation: The expression of SARS-CoV genes is tightly regulated by viral and host factors. Transcription factors such as SP1 and HNF4α play crucial roles in regulating the expression of the ACE2 receptor, which is essential for viral entry .
Post-Translational Modifications: SARS-CoV proteins undergo various post-translational modifications, including phosphorylation, glycosylation, and ubiquitination, which are critical for viral replication and immune evasion .
Biomedical Research: SARS-CoV has been extensively studied to understand viral pathogenesis and host immune responses. This research has led to the development of diagnostic tools and therapeutic strategies .
Diagnostic Tools: Techniques such as polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) are used to detect SARS-CoV infection .
Therapeutic Strategies: Antiviral drugs, monoclonal antibodies, and vaccines have been developed to combat SARS-CoV infection. These therapeutic strategies target various stages of the viral life cycle .
Throughout the Life Cycle: SARS-CoV plays a critical role in the viral life cycle, from initial infection to replication and assembly of new virions. The virus hijacks the host’s cellular machinery to produce viral RNA and proteins, leading to the assembly of new virions . The N protein of SARS-CoV is essential for packaging the viral RNA into new virions and facilitating their release from host cells .
