Viral Antigens
Product List
CoV-2-S1 (319-541), Fc
Coronavirus 2019 Spike Glycoprotein-S1 Receptor Binding Domain (319-541 a.a), Fc Recombinant
This product consists of the Receptor Binding Domain (RBD) of the SARS-CoV-2 Spike Glycoprotein S1, derived from the Wuhan-Hu-1 strain (amino acids 319-541). It is recombinantly produced in HEK293 cells and features a C-terminal Fc tag for purification and detection purposes.
HEK293 Cells.
The product is provided as a lyophilized powder, which means it has been freeze-dried to remove moisture for stability.
CoV-2-Spike (1-1211)
Coronavirus 2019 Spike (1-1211 a.a.), Recombinant
This recombinant protein, expressed in CHO cells, encompasses the full-length spike protein of the Wuhan-Hu-1 strain of Coronavirus 2019 (amino acids 1-1211). It has a molecular weight of 134 kDa and includes a C-terminal His tag. Modifications include a mutation of the furin cleavage site from RRAR to SRAS and the replacement of the transmembrane domain and intravirion part with a glycine-serine linker followed by the His-tag.
CHO
CoV-2-Spike (1-260)
Coronavirus 2019 Spike (1-260 a.a.), Recombinant
This recombinant protein is derived from HEK293 cells and encompasses the N-terminal domain (amino acids 1-260) of the Wuhan-Hu-1 strain of the Coronavirus 2019 Spike protein. It is fused with an Fc tag at its C-terminus.
HEK293
CoV-NL63
Coronavirus NL63 Recombinant
This recombinant Coronavirus NL63 nucleoprotein, expressed in E. coli, encompasses amino acids 221 to 340. A 6-amino acid His tag is fused to the C-terminal, resulting in a total of 130 amino acids.
SARS Core (1-49,192-220 a.a.)
SARS-Associated Coronavirus Nucleocapsid Core Recombinant
CoV HKU1 Human
Coronavirus HKU1 Nucleoprotein Human Recombinant
Recombinant Human Coronavirus HKU1 Nucleoprotein is a full-length protein expressed in E. coli bacteria. The protein lacks the first 30 amino acids that make up the predicted signal peptide and has a molecular weight of 50 kDa. It includes a 6xHis tag attached to the C-terminus to facilitate purification using a proprietary chromatographic technique.
Escherichia Coli.
CoV OC43 Human
Coronavirus OC43 Nucleoprotein Human Recombinant
Recombinant Human Coronavirus OC43 Nucleoprotein is a full-length protein expressed in E. coli. The predicted signal peptide of the first 30 amino acids is not present in this recombinant protein. The protein migrates at 50kDa.
The CoV OC43 protein was designed with a C-terminal 6xHis tag and is purified using a proprietary chromatographic technique.
Escherichia Coli.
CoV-2 S1 (16-685)
Coronavirus 2019 Spike Glycoprotein-S1 (16-685 a.a.), Recombinant
This product features the recombinant Coronavirus 2019 (COVID-19) Spike Glycoprotein S1 subunit, specifically from the Wuhan-Hu-1 strain. This protein encompasses amino acids 16 to 685, resulting in a molecular weight of 76.2 kDa. A 6-histidine tag is present at the C-terminus. The protein is purified using advanced chromatographic methods.
HEK293 Cells.
CoV-2 S1 (16-685), Biotin
Coronavirus 2019 Spike Glycoprotein-S1 (16-685 a.a.), Biotinylated Recombinant
This product consists of a biotinylated recombinant protein derived from HEK293 cells. The protein comprises amino acids 16 to 685 of the Wuhan-Hu-1 strain of the Coronavirus 2019 (CoV-2) Spike Glycoprotein S1, fused with a His tag and an Avi-tag at the C-terminal end.
HEK293 Cells.
CoV-2 S1 (16-685), Fc
Coronavirus 2019 Spike Glycoprotein-S1 (16-685 a.a.), Fc Recombinant
This recombinant protein, expressed in HEK293 cells, encompasses amino acids 16 to 685 of the Spike Glycoprotein S1 subunit from the Wuhan-Hu-1 strain of Coronavirus 2019 (CoV-2). It is fused with an Fc tag at the C-terminus.
HEK293 Cells.
Introduction
Severe Acute Respiratory Syndrome (SARS) is a viral respiratory illness caused by a coronavirus known as SARS-CoV. It was first identified in 2003 during an outbreak that began in China and spread to other countries . SARS-CoV belongs to the family Coronaviridae, which is divided into four genera: Alpha, Beta, Gamma, and Delta coronaviruses . SARS-CoV is classified under the Betacoronavirus genus.
Key Biological Properties: SARS-CoV is an enveloped, positive-sense, single-stranded RNA virus. It has a crown-like appearance due to spike proteins on its surface .
Expression Patterns and Tissue Distribution: SARS-CoV primarily infects the respiratory tract, but it can also affect other organs such as the gastrointestinal tract, liver, and kidneys . The virus binds to the angiotensin-converting enzyme 2 (ACE2) receptor, which is widely distributed in various tissues, including the lungs, heart, and intestines .
Primary Biological Functions: The primary function of SARS-CoV is to replicate within host cells. The virus hijacks the host’s cellular machinery to produce viral RNA and proteins, leading to the assembly of new virions .
Role in Immune Responses and Pathogen Recognition: SARS-CoV triggers an immune response by activating various immune cells and signaling pathways. The spike protein of the virus is recognized by the host’s immune system, leading to the production of neutralizing antibodies .
Mechanisms with Other Molecules and Cells: SARS-CoV enters host cells by binding to the ACE2 receptor and undergoing proteolytic cleavage by host cell proteases such as TMPRSS2 . This facilitates viral entry and fusion with the host cell membrane.
Binding Partners and Downstream Signaling Cascades: The binding of SARS-CoV to ACE2 triggers downstream signaling cascades that modulate immune responses and inflammation. The virus can also evade immune detection by interfering with interferon signaling pathways .
Transcriptional Regulation: The expression of SARS-CoV genes is tightly regulated by viral and host factors. Transcription factors such as SP1 and HNF4α play crucial roles in regulating the expression of the ACE2 receptor, which is essential for viral entry .
Post-Translational Modifications: SARS-CoV proteins undergo various post-translational modifications, including phosphorylation, glycosylation, and ubiquitination, which are critical for viral replication and immune evasion .
Biomedical Research: SARS-CoV has been extensively studied to understand viral pathogenesis and host immune responses. This research has led to the development of diagnostic tools and therapeutic strategies .
Diagnostic Tools: Techniques such as polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) are used to detect SARS-CoV infection .
Therapeutic Strategies: Antiviral drugs, monoclonal antibodies, and vaccines have been developed to combat SARS-CoV infection. These therapeutic strategies target various stages of the viral life cycle .
Throughout the Life Cycle: SARS-CoV plays a critical role in the viral life cycle, from initial infection to replication and assembly of new virions. The virus hijacks the host’s cellular machinery to produce viral RNA and proteins, leading to the assembly of new virions . The N protein of SARS-CoV is essential for packaging the viral RNA into new virions and facilitating their release from host cells .
