Viral Antigens
Product List
SARS MERS, Sf9
SARS MERS Spike Recombinant
Recombinant SARS MERS protein, expressed in Sf9 insect cells using a baculovirus system, is a single, glycosylated polypeptide chain. This protein consists of 1285 amino acids (spanning from positions 18 to 1296), resulting in a molecular weight of 141.6 kDa. A 6-amino acid His-tag is fused to the C-terminus of the SARS MERS protein to facilitate purification, which is achieved through proprietary chromatographic methods.
Sf9, Baculovirus cells.
SARS MERS, Sf9 Active
SARS MERS Spike Recombinant, Sf9 Active
SARS Mosaic S(C)
SARS-Associated Coronavirus Spike Mosaic S(C) Recombinant
SARS Spike (306-515)
Coronavirus Spike Receptor Binding Domain (306-515 a.a.), Recombinant
This recombinant SARS Spike protein consists of 220 amino acids (spanning positions 306 to 515) and has a molecular weight of 24.8 kDa. A 6 amino acid His-tag is fused to the C-terminus to facilitate purification, which is achieved using proprietary chromatographic methods.
HEK293 Cells.
SARS Spike (306-515), Sf9
Coronavirus Spike Receptor Binding Domain (306-515 a.a.)Recombinant, Sf9
This part details the characteristics of the SARS Spike protein produced using Sf9 Baculovirus cells, including its amino acid length, molecular weight, attached tag for purification, and purification method.
Sf9, Baculovirus cells.
SARS MERS, (18-751)
SARS MERS Spike S1 (18-751 a.a.) Recombinant
SARS MERS Recombinant, produced using Sf9 insect cells and Baculovirus expression system, is a single, glycosylated polypeptide chain. This protein consists of 740 amino acids (spanning from position 18 to 751), resulting in a molecular weight of 82.0kDa. For purification and detection purposes, a 6 amino acid His-tag is fused to the C-terminus of the SARS MERS protein. Purification is achieved through proprietary chromatographic techniques.
Sf9, Baculovirus cells.
SARS MERS, HEK
SARS MERS Spike Glycoprotein-S1, Recombinant
SARS Spike (14-667)
SARS Spike (14-667 a.a.), Recombinant
SARS Spike (1-53, 90-115, 171-205)
SARS-Associated Coronavirus Spike (1-53, 90-115, 171-205 a.a.), Recombinant
SARS Spike (1-666)
SARS Spike (1-666 a.a.), Recombinant
This recombinant protein, derived from HEK293 cells, consists of the SARS Coronavirus Spike S1 Glycoprotein (amino acids 1-666) with a C-terminal His tag.
Introduction
Severe Acute Respiratory Syndrome (SARS) is a viral respiratory illness caused by a coronavirus known as SARS-CoV. It was first identified in 2003 during an outbreak that began in China and spread to other countries . SARS-CoV belongs to the family Coronaviridae, which is divided into four genera: Alpha, Beta, Gamma, and Delta coronaviruses . SARS-CoV is classified under the Betacoronavirus genus.
Key Biological Properties: SARS-CoV is an enveloped, positive-sense, single-stranded RNA virus. It has a crown-like appearance due to spike proteins on its surface .
Expression Patterns and Tissue Distribution: SARS-CoV primarily infects the respiratory tract, but it can also affect other organs such as the gastrointestinal tract, liver, and kidneys . The virus binds to the angiotensin-converting enzyme 2 (ACE2) receptor, which is widely distributed in various tissues, including the lungs, heart, and intestines .
Primary Biological Functions: The primary function of SARS-CoV is to replicate within host cells. The virus hijacks the host’s cellular machinery to produce viral RNA and proteins, leading to the assembly of new virions .
Role in Immune Responses and Pathogen Recognition: SARS-CoV triggers an immune response by activating various immune cells and signaling pathways. The spike protein of the virus is recognized by the host’s immune system, leading to the production of neutralizing antibodies .
Mechanisms with Other Molecules and Cells: SARS-CoV enters host cells by binding to the ACE2 receptor and undergoing proteolytic cleavage by host cell proteases such as TMPRSS2 . This facilitates viral entry and fusion with the host cell membrane.
Binding Partners and Downstream Signaling Cascades: The binding of SARS-CoV to ACE2 triggers downstream signaling cascades that modulate immune responses and inflammation. The virus can also evade immune detection by interfering with interferon signaling pathways .
Transcriptional Regulation: The expression of SARS-CoV genes is tightly regulated by viral and host factors. Transcription factors such as SP1 and HNF4α play crucial roles in regulating the expression of the ACE2 receptor, which is essential for viral entry .
Post-Translational Modifications: SARS-CoV proteins undergo various post-translational modifications, including phosphorylation, glycosylation, and ubiquitination, which are critical for viral replication and immune evasion .
Biomedical Research: SARS-CoV has been extensively studied to understand viral pathogenesis and host immune responses. This research has led to the development of diagnostic tools and therapeutic strategies .
Diagnostic Tools: Techniques such as polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) are used to detect SARS-CoV infection .
Therapeutic Strategies: Antiviral drugs, monoclonal antibodies, and vaccines have been developed to combat SARS-CoV infection. These therapeutic strategies target various stages of the viral life cycle .
Throughout the Life Cycle: SARS-CoV plays a critical role in the viral life cycle, from initial infection to replication and assembly of new virions. The virus hijacks the host’s cellular machinery to produce viral RNA and proteins, leading to the assembly of new virions . The N protein of SARS-CoV is essential for packaging the viral RNA into new virions and facilitating their release from host cells .
