Viral Antigens
Product List
CoV-2 S1 (1-681)
Coronavirus 2019-nCoV Spike Glycoprotein-S1 (1-681), Recombinant
This recombinant protein, derived from HEK293 cells, consists of the S1 subunit of the Coronavirus 2019-nCoV Spike Glycoprotein (Wuhan-Hu-1 strain). It encompasses amino acids 1 to 681 and is fused with an Fc tag at the C-terminus, resulting in a molecular weight of 76 kDa.
HEK293.
The product is a clear solution that has undergone sterile filtration.
SARS Spike (306-527)
SARS Spike Receptor Binding Domain(306-527 a.a.), Recombinant
SARS Spike (408-470, 540-573)
SARS-Associated Coronavirus Spike (408-470, 540-573 a.a.), Recombinant
This recombinant protein, derived from E.coli, encompasses the immunodominant regions of the Spike protein (amino acids 408-470 and 540-573) and is fused with a 6xHis tag at the C-terminal end.
SARS Spike Mosaic
SARS-Associated Coronavirus Spike Mosaic, Recombinant
This recombinant protein, derived from E. coli, encompasses amino acids 20-210 of the SARS Spike protein's immunodominant region. It is fused with a 6xHis tag at its C-terminus.
SARS MERS
SARS MERS Spike S1 Recombinant
Purification of SARS MERS is achieved through a proprietary chromatographic technique.
SARS MERS RBD
SARS MERS Spike Receptor Binding Domain Recombinant
SARS MERS RBD Recombinant, expressed in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain. It consists of 258 amino acids (spanning positions 358 to 606) and has a molecular weight of 28.2kDa. A 6 amino acid His-tag is fused to the C-terminus of the SARS MERS RBD. Purification is achieved using proprietary chromatographic techniques.
Sf9, Baculovirus cells.
SARS MERS RBD, Active
SARS MERS Spike Receptor Binding Domain Recombinant, Active
SARS MERS S2
SARS MERS Spike S2 Recombinant
SARS MERS S2 Recombinant, expressed using Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain. It comprises 554 amino acids (752-1296aa), resulting in a molecular weight of 60.7kDa. The protein is engineered with a 6 amino acid His-tag at the C-terminus and undergoes a purification process using specialized chromatographic methods.
Sf9, Baculovirus cells.
SARS Nucleocapsid, 1-49 a.a.
SARS-Associated Coronavirus Nuclecapsid (1-49 a.a.), Recombinant
This recombinant protein is derived from E. coli and encompasses amino acids 1 to 49 of the Nucleocapsid protein, representing immunodominant regions.
SARS Nucleocaspid (2-422), HEK
SARS Nucleocaspid (2-422 a.a.), HEK Recombinant
This recombinant protein, derived from HEK293 cells, comprises the SARS Coronavirus Nucleoprotein (amino acids 2-422) with a 6-His tag fused to its N-terminal end.
HEK293
Introduction
Severe Acute Respiratory Syndrome (SARS) is a viral respiratory illness caused by a coronavirus known as SARS-CoV. It was first identified in 2003 during an outbreak that began in China and spread to other countries . SARS-CoV belongs to the family Coronaviridae, which is divided into four genera: Alpha, Beta, Gamma, and Delta coronaviruses . SARS-CoV is classified under the Betacoronavirus genus.
Key Biological Properties: SARS-CoV is an enveloped, positive-sense, single-stranded RNA virus. It has a crown-like appearance due to spike proteins on its surface .
Expression Patterns and Tissue Distribution: SARS-CoV primarily infects the respiratory tract, but it can also affect other organs such as the gastrointestinal tract, liver, and kidneys . The virus binds to the angiotensin-converting enzyme 2 (ACE2) receptor, which is widely distributed in various tissues, including the lungs, heart, and intestines .
Primary Biological Functions: The primary function of SARS-CoV is to replicate within host cells. The virus hijacks the host’s cellular machinery to produce viral RNA and proteins, leading to the assembly of new virions .
Role in Immune Responses and Pathogen Recognition: SARS-CoV triggers an immune response by activating various immune cells and signaling pathways. The spike protein of the virus is recognized by the host’s immune system, leading to the production of neutralizing antibodies .
Mechanisms with Other Molecules and Cells: SARS-CoV enters host cells by binding to the ACE2 receptor and undergoing proteolytic cleavage by host cell proteases such as TMPRSS2 . This facilitates viral entry and fusion with the host cell membrane.
Binding Partners and Downstream Signaling Cascades: The binding of SARS-CoV to ACE2 triggers downstream signaling cascades that modulate immune responses and inflammation. The virus can also evade immune detection by interfering with interferon signaling pathways .
Transcriptional Regulation: The expression of SARS-CoV genes is tightly regulated by viral and host factors. Transcription factors such as SP1 and HNF4α play crucial roles in regulating the expression of the ACE2 receptor, which is essential for viral entry .
Post-Translational Modifications: SARS-CoV proteins undergo various post-translational modifications, including phosphorylation, glycosylation, and ubiquitination, which are critical for viral replication and immune evasion .
Biomedical Research: SARS-CoV has been extensively studied to understand viral pathogenesis and host immune responses. This research has led to the development of diagnostic tools and therapeutic strategies .
Diagnostic Tools: Techniques such as polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) are used to detect SARS-CoV infection .
Therapeutic Strategies: Antiviral drugs, monoclonal antibodies, and vaccines have been developed to combat SARS-CoV infection. These therapeutic strategies target various stages of the viral life cycle .
Throughout the Life Cycle: SARS-CoV plays a critical role in the viral life cycle, from initial infection to replication and assembly of new virions. The virus hijacks the host’s cellular machinery to produce viral RNA and proteins, leading to the assembly of new virions . The N protein of SARS-CoV is essential for packaging the viral RNA into new virions and facilitating their release from host cells .
